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WEE1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1753426

Wee1 is a nuclear kinase belonging to the Ser/Thr family of protein kinases in the fission yeast Schizosaccharomyces pombe (S.pombe). It is mapped to 111p15.4. This gene encodes a nuclear protein which is a tyrosine kinase beloning to the Ser/Thr femily of… More details

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Product Overview

Online Datasheet in PDF
Product Name
WEE1, Polyclonal Antibody
Full Product Name
Anti-WEE1 Antibody
Product Gene Name
anti-WEE1 antibody
Product Synonym Names
WEE1; Wee1-like protein kinase; WEE1hu; EC 2. 7. 10. 2; Wee1A kinase; WEE1 G2 checkpoint kinase
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Lyophilized. Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for 6 months. Avoid repeated freezing and thawing.
Chromosome Location
Chromosome: 11; NC_000011.9 (9595228..9611314). Location: 11p15.3-p15.1
OMIM
193525
3D Structure
ModBase 3D Structure for P30291
Immunogen
E Coli-derived human WEE1 recombinant protein (Position: R13-R581).
Reconstitution
0.2ml of distilled water will yield a concentration of 500ug/ml.
Relavent Detection Systems
Lab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-WEE1 antibody
Western Blot (WB), Flow Cytometry (FC), Direct ELISA (EIA)
Application Notes for anti-WEE1 antibody
WB: Concentration: 0.25-0.5ug/ml; Tested Species: Human, Mouse
FC: Concentration: 1-3ug/1x106 cells; Tested Species: Human
Direct ELISA: Concentration: 0.1-0.5ug/ml; Tested Species: Human

Tested Species: In-house tested species with positive results.
Other applications have not been tested.
Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-WEE1 antibody

Figure 1. Western blot analysis of WEE1 using anti-WEE1 antibody (MBS1753426).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates
Lane 2: human K562 whole cell lysates
Lane 3: mouse lung tissue lysates
Lane 4: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-WEE1 antigen affinity purified polyclonal antibody (Catalog # MBS1753426) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for WEE1 at approximately 72KD. The expected band size for WEE1 is at 72KD.
Rabbit Anti-WEE1 Polyclonal Antibody – Cat# MBS1753426

Flow Cytometry (FC/FACS) of anti-WEE1 antibody

Figure 2. Flow Cytometry analysis of A549 cells using anti-WEE1 antibody (MBS1753426).
Overlay histogram showing A549 cells stained with MBS1753426 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WEE1 Antibody (MBS1753426, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-WEE1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1753426

Product References and Citations for anti-WEE1 antibody

1. Heald, R., McLoughlin, M., McKeon, F. Human wee1 maintains mitotic timing by protecting the nucleus from cytoplasmically activated cdc2 kinase. Cell 74: 463-474, 1993.
2. Igarashi, M., Nagata, A., Jinno, S., Suto, K., Okayama, H. Wee1(+)-like gene in human cells. Nature 353: 80-83, 1991.
3. Matsuo, T., Yamaguchi, S., Mitsui, S., Emi, A., Shimoda, F., Okamura, H. Control mechanism of the circadian clock for timing of cell division in vivo. Science 302: 255-259, 2003.

Other Notes

Small volumes of anti-WEE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WEE1 purchase

MBS1753426 is an antibody targeting WEE1. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for WEE1. It may not necessarily be applicable to this product.

NCBI GI #
221307502
NCBI GeneID
7465
NCBI Accession #
NP_001137448.1
UniProt Primary Accession #
P30291
UniProt Secondary Accession #
B3KVE1; D3DQV0
UniProt Related Accession #
P30291
GenBank Nucleotide #
NM_001143976.1
NCBI Official Full Name
wee1-like protein kinase isoform 2
NCBI Official Synonym Full Names
WEE1 homolog (S. pombe)
NCBI Official Symbol
WEE1
NCBI Official Synonym Symbols
WEE1A; WEE1hu
UniProt Protein Name
Wee1-like protein kinase
UniProt Synonym Protein Names
Wee1A kinase
Protein Family
Mitosis inhibitor protein kinase
UniProt Gene Name
WEE1
UniProt Synonym Gene Names
WEE1hu
UniProt Entry Name
WEE1_HUMAN
NCBI Protein Information
wee1-like protein kinase; wee1A kinase; WEE1+ homolog

NCBI Summary for WEE1

This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]
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UniProt Comments for WEE1

Wee1: a protein kinase of the WEE family. A nuclear protein which catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.10.2; Kinase, protein; Protein kinase, Other; Other group; WEE family

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; magnesium ion binding; ATP binding

Biological Process: mitosis; peptidyl-tyrosine phosphorylation; establishment of cell polarity; regulation of cell cycle; cell division; microtubule cytoskeleton organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; blood coagulation; neurite morphogenesis; G1/S transition of mitotic cell cycle
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Research Articles on WEE1

1. These data support the hypothesis that Cdc14A counteracts Cdk1-cyclin B1 activity through Wee1 dephosphorylation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-WEE1 antibody

 Reference Product  PubMed Publications
 CDK1 antibody  >106 publications with WEE1 and CDK1
 CDK2 antibody  >39 publications with WEE1 and CDK2
 CASP3 antibody  >28 publications with WEE1 and CASP3
 PLK1 antibody  >22 publications with WEE1 and PLK1
 SKP2 antibody  >6 publications with WEE1 and SKP2
 CRK antibody  >3 publications with WEE1 and CRK
 KLF2 antibody  >2 publications with WEE1 and KLF2
 SMURF1 antibody  >1 publications with WEE1 and SMURF1
 BRSK2 antibody  >1 publications with WEE1 and BRSK2
 BRSK1 antibody  >1 publications with WEE1 and BRSK1

Pathways associated with anti-WEE1 antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chk1/Chk2(Cds1) Mediated Inactivation Of Cyclin B:Cdk1 Complex Pathway antibodies  Chk1/Chk2(Cds1) Mediated Inactivation Of Cyclin B:Cdk1 Complex Pathway Diagram
 Cyclin A/B1 Associated Events During G2/M Transition Pathway antibodies  Cyclin A/B1 Associated Events During G2/M Transition Pathway Diagram
 Cyclin A:Cdk2-associated Events At S Phase Entry Pathway antibodies  Cyclin A:Cdk2-associated Events At S Phase Entry Pathway Diagram
 Cyclin E Associated Events During G1/S Transition Pathway antibodies  Cyclin E Associated Events During G1/S Transition Pathway Diagram

Diseases associated with anti-WEE1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >121 publications with WEE1 and Neoplasms
 Carcinoma Antibodies  >39 publications with WEE1 and Carcinoma
 Breast Neoplasms Antibodies  >18 publications with WEE1 and Breast Neoplasms
 Drug Toxicity Antibodies  >16 publications with WEE1 and Drug Toxicity
 Neoplasms, Experimental Antibodies  >12 publications with WEE1 and Neoplasms, Experimental
 Adenocarcinoma Antibodies  >10 publications with WEE1 and Adenocarcinoma
 Liver Diseases Antibodies  >9 publications with WEE1 and Liver Diseases
 Carcinoma, Squamous Cell Antibodies  >9 publications with WEE1 and Carcinoma, Squamous Cell
 Liver Neoplasms Antibodies  >7 publications with WEE1 and Liver Neoplasms
 Cell Transformation, Neoplastic Antibodies  >5 publications with WEE1 and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-WEE1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >19 publications with WEE1 and Liver
 Blood Antibodies  >17 publications with WEE1 and Blood
 Brain Antibodies  >13 publications with WEE1 and Brain
 Prostate Antibodies  >10 publications with WEE1 and Prostate
 Ovary Antibodies  >9 publications with WEE1 and Ovary
 Bone Antibodies  >9 publications with WEE1 and Bone
 Embryonic Tissue Antibodies  >8 publications with WEE1 and Embryonic Tissue
 Lung Antibodies  >8 publications with WEE1 and Lung
 Kidney Antibodies  >7 publications with WEE1 and Kidney
 Vascular Antibodies  >7 publications with WEE1 and Vascular
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