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IREB2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9213792

IREB2 is a RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE… More details

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Product Overview

Online Datasheet in PDF
Product Name
IREB2, Polyclonal Antibody
Full Product Name
IREB2 Antibody (Center)
Product Gene Name
anti-IREB2 antibody
Product Synonym Names
Iron-responsive element-binding protein 2; IRE-BP 2; Iron regulatory protein 2; IRP2; IREB2
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human (Predicted Reactivity: Mouse, Pig, Rat)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Specificity
This IREB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 201-230 amino acids from the Central region of human IREB2.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
IREB2 peptide (MBS9219307) is used for blocking the activity of IREB2 antibody (MBS9213792)
Immunogen Sequence Positions
201-230
OMIM
147582
3D Structure
ModBase 3D Structure for P48200
Calculated MW
105045

Product Categories/Family for anti-IREB2 antibody

Cardiovascular; Metabolism
Applications Tested/Suitable for anti-IREB2 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS)
Application Notes for anti-IREB2 antibody
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50

Western Blot (WB) of anti-IREB2 antibody

IREB2 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IREB2 antibody detected the IREB2 protein (arrow).
Rabbit Anti-IREB2 Polyclonal Antibody – Cat# MBS9213792

Immunohistochemistry (IHC) of anti-IREB2 antibody

IREB2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IREB2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-IREB2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9213792

Flow Cytometry (FC/FACS) of anti-IREB2 antibody

IREB2 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-IREB2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS9213792

Product References and Citations for anti-IREB2 antibody

Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)
Hansen, H.M., et al. Hum. Mol. Genet. 19(18):3652-3661(2010)
Cho, M.H., et al. Nat. Genet. 42(3):200-202(2010)
Salahudeen, A.A., et al. Science 326(5953):722-726(2009)
DeMeo, D.L., et al. Am. J. Hum. Genet. 85(4):493-502(2009)

Other Notes

Small volumes of anti-IREB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IREB2 purchase

MBS9213792 is an antibody targeting IREB2. Also known as IREB2 Antibody (Center). Reactive in Human (Predicted Reactivity: Mouse, Pig, Rat). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IREB2. It may not necessarily be applicable to this product.

NCBI GI #
41352693
NCBI GeneID
3658
NCBI Accession #
NP_004127.1
UniProt Primary Accession #
P48200
UniProt Secondary Accession #
Q13095; Q1HE21; Q59FQ7; Q8WVK6; Q9UF17; A8KAC7; E1CJT9; H0YKU0
UniProt Related Accession #
P48200
GenBank Nucleotide #
NM_004136.2
NCBI Official Full Name
iron-responsive element-binding protein 2
NCBI Official Synonym Full Names
iron-responsive element binding protein 2
NCBI Official Symbol
IREB2
NCBI Official Synonym Symbols
ACO3; IRP2; IRP2AD
UniProt Protein Name
Iron-responsive element-binding protein 2
UniProt Synonym Protein Names
Iron regulatory protein 2; IRP2
Protein Family
Iron-responsive element-binding protein
UniProt Gene Name
IREB2
UniProt Synonym Gene Names
IRE-BP 2; IRP2
UniProt Entry Name
IREB2_HUMAN
NCBI Protein Information
iron-responsive element-binding protein 2

UniProt Comments for IREB2

IREB2: RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'- UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. Belongs to the aconitase/IPM isomerase family.

Protein type: Translation; RNA-binding

Chromosomal Location of Human Ortholog: 15q25.1

Cellular Component: mitochondrion; cytoplasm; cytosol

Molecular Function: protein binding; iron-responsive element binding; translation repressor activity; RNA binding; 4 iron, 4 sulfur cluster binding; metal ion binding

Biological Process: erythrocyte homeostasis; response to retinoic acid; cellular iron ion homeostasis; negative regulation of translation; osteoclast differentiation; response to iron(II) ion; protoporphyrinogen IX biosynthetic process; iron ion transport; intestinal absorption; post-embryonic development; aging
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Research Articles on IREB2

1. IRP2 can regulate the expression of TfR and Fn by changing its own protein expression and thereby regulate iron metabolism.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IREB2 antibody

 Reference Product  PubMed Publications
 MAX antibody  >1 publications with IREB2 and MAX

Pathways associated with anti-IREB2 antibody

 Products by Pathway  Pathway Diagram
 Bacterial Secretion System Pathway antibodies  Bacterial Secretion System Pathway Diagram
 Bacterial Secretion System Pathway antibodies  Bacterial Secretion System Pathway Diagram

Diseases associated with anti-IREB2 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >4 publications with IREB2 and Nervous System Diseases
 Lung Neoplasms Antibodies  >4 publications with IREB2 and Lung Neoplasms
 Brain Diseases Antibodies  >4 publications with IREB2 and Brain Diseases
 Anemia Antibodies  >2 publications with IREB2 and Anemia
 Hypertension Antibodies  >1 publications with IREB2 and Hypertension
 Liver Diseases Antibodies  >1 publications with IREB2 and Liver Diseases

Organs/Tissues associated with anti-IREB2 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >13 publications with IREB2 and Lung
 Brain Antibodies  >6 publications with IREB2 and Brain
 Blood Antibodies  >3 publications with IREB2 and Blood
 Muscle Antibodies  >1 publications with IREB2 and Muscle
 Intestine Antibodies  >1 publications with IREB2 and Intestine
 Vascular Antibodies  >1 publications with IREB2 and Vascular
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