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ADAMTS5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8577029

Metalloproteinase that plays an important role in connective tissue organization, development, inflammation, arthritis, and cell migration. ADAMTS5 is an extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of… More details

$430 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ADAMTS5, Polyclonal Antibody
Full Product Name
ADAMTS5
Product Gene Name
Product Synonym Names
ADAMTS5; A disintegrin and metalloproteinase with thrombospondin motifs 5; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5; A Disintigrin And Metalloproteinase with ThromboSpondin motif-5; ADAM metallopeptidase with thrombospondin type 1 motif 5; ADAM TS 11; ADAM TS 5; ADAM TS5; ADAMTS 11; ADAMTS 5; ADAMTS11; ADMP 2; ADMP2; Aggrecanase 2; aggrecanase-2; FLJ36738; Implantin; ThromboSpondin motif-5
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Purity/Purification
Affinity purified by Protein A
Preparation and Storage
Store at -20 degree C for one year. Avoid repeated freeze/thaw cycles.
The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 degree C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 degree C.
Molecular Weight
102 kDa
3D Structure
ModBase 3D Structure for Q9UNA0
Immunogen
KLH conjugated synthetic peptide derived from human Aggrecan 2 511-610/930
Positive Control
Expressed at low level in placenta primarily but also detected in heart and brain, cervix,uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient.
Subcellular Location
Secreted, extracellular space, extracellular matrix.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ADAMTS5 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF-Tissue)
Application Notes for anti-ADAMTS5 antibody
Recommended Dilutions:
WB 1:500-2000
IHC-P 1:100-500
IF-Tissue 1:100-500: 1:5000-10000

Western Blot (WB) of anti-ADAMTS5 antibody

Sample: Lane 1: Mouse Placenta tissue lysates.
Lane 2: Mouse Spleen tissue lysates.
Lane 3: Human U87MG cell lysates.
Lane 4: Human U251 cell lysates.
Lane 5: Human SH-SY5Y cell lysates.
Lane 6: Human HeLa cell lysates.
Primary: Anti- ADAMTS5 at 1/1000 dilution.
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution.
Predicted band size: 102 kDa.
Observed band size: 102 kDa.
Rabbit Anti-ADAMTS5 Polyclonal Antibody – Cat# MBS8577029

Immunohistochemistrty (IHC) of anti-ADAMTS5 antibody

Tissue: rat brain tissue; 4% Paraformaldehyde-fixed and paraffinembedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37 degrees C for 20 min; Incubation: Anti-Aggrecanase-2/ADAMTS5 Polyclonal Antibody, Unconjugated 1:200, overnight at 4 degrees C, followed by conjugation to the secondary antibody and DAB staining.
Rabbit Anti-ADAMTS5 Polyclonal Antibody, Immunohistochemistrty (IHC) – Cat# MBS8577029

Other Notes

Small volumes of anti-ADAMTS5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ADAMTS5 purchase

MBS8577029 is an antibody targeting ADAMTS5. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ADAMTS5. It may not necessarily be applicable to this product.

UniProt Primary Accession #

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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