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APC Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9504535

MBS9504535 is an antibody targeting APC. Also known as APC Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$300 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
APC, Polyclonal Antibody
Full Product Name
APC Antibody
Product Gene Name
Product Synonym Names
Adenomatous polyposis coli protein; adenomatous polyposis of the colon gene; APC; APC protein; DP2.5; mAPC
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Concentration
1 mg/ml (lot specific)
Specificity
APC Antibody detects endogenous levels of total APC protein.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Molecular Weight
311 kDa
OMIM
M73548 mRNA
3D Structure
ModBase 3D Structure for P25054
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human APC.
Immunogen Range
2794-2843
Applications Tested/Suitable for anti-APC antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-APC antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:40000

Immunohistochemistry (IHC) of anti-APC antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using APC Antibody. The picture on the right is blocked with the synthesized peptide.
Rabbit Anti-APC Polyclonal Antibody – Cat# MBS9504535

Western Blot (WB) of anti-APC antibody

Western blot analysis of lysates from COLO205 cells, using APC Antibody. The lane on the right is blocked with the synthesized peptide.
Rabbit Anti-APC Polyclonal Antibody, Western Blot (WB) – Cat# MBS9504535

Other Notes

Small volumes of anti-APC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for APC. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
adenomatous polyposis coli protein isoform b
NCBI Official Synonym Full Names
APC, WNT signaling pathway regulator
NCBI Official Symbol
APC
NCBI Official Synonym Symbols
GS; DP2; DP3; BTPS2; DP2.5; PPP1R46
UniProt Protein Name
Adenomatous polyposis coli protein
UniProt Synonym Protein Names
Deleted in polyposis 2.5
UniProt Gene Name
APC
UniProt Synonym Gene Names
Protein APC
NCBI Protein Information
adenomatous polyposis coli protein

NCBI Summary for APC

This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]
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UniProt Comments for APC

Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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