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ATF4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758067

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ATF4, Polyclonal Antibody
Full Product Name
Anti-ATF4 Antibody Picoband
Product Gene Name
anti-ATF4 antibody
Product Synonym Names
Cyclic AMP-dependent transcription factor ATF-4; activating transcription factor 4 (tax-responsive enhancer element B67); Cyclic AMP-dependent transcription factor ATF-4; cAMP-dependent transcription factor ATF-4; Activating transcription factor 4; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; Tax-responsive enhancer element-binding protein 67; TaxREB67; ATF4; CREB2; TXREB
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. NotExpressed in the platelets.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
40 kDa
Chromosome Location
Chromosome: 22; NC_000022.10 (39916569..39918691). Location: 22q13.1
OMIM
604064
3D Structure
ModBase 3D Structure for P18848
Immunogen
E coli-derived human ATF4 recombinant protein (Position: E10-E210).
Subcellular Localization
Cytoplasm
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Transcriptional activator. Binds the cAMP response element (CRE)(consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production (By similarity). It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Regulates the induction of DDIT3/CHOP and asparagine synthetase (ASNS) in response to endoplasmic reticulum (ER) stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. During ER stress response, activates the transcription of NLRP1, possibly in concert with other factors (PubMed:26086088).

Product Categories/Family for anti-ATF4 antibody

Primary Antibodies; Domain Families; Epigenetics and Nuclear Signaling; Hlh/Leucine Zipper; Transcription
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ATF4 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-ATF4 antibody
WB: 0.25-0.5ug/ml, Human, Mouse, Rat
ICC/IF: 5ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-ATF4 antibody

Figure 1. Western blot analysis of ATF4 using anti-ATF4 antibody (MBS1758067).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF4 antigen affinity purified polyclonal antibody (#MBS1758067) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for ATF4 at approximately 45-50 kDa. The expected band size for ATF4 is at 38,39,45-50 kDa.
Rabbit Anti-ATF4 Polyclonal Antibody – Cat# MBS1758067

Immunofluorescence (IF) of anti-ATF4 antibody

Figure 2. IF analysis of ATF4 using anti-ATF4 antibody (MBS1758067) and anti-Tubulin Alpha antibody (M03989-3).
ATF4 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (#MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-ATF4 Antibody (MBS1758067) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Rabbit Anti-ATF4 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1758067

Flow Cytometry (FC/FACS) of anti-ATF4 antibody

Figure 3. Flow Cytometry analysis of SiHa cells using anti-ATF4 antibody (MBS1758067).
Overlay histogram showing SiHa cells stained with MBS1758067 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATF4 Antibody (MBS1758067, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-ATF4 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758067

Product References and Citations for anti-ATF4 antibody

1. De Angelis, R., Iezzi, S., Bruno, T., Corbi, N., Di Padova, M., Floridi, A., Fanciulli, M., Passananti, C.Functional interaction of the subunit 3 of RNA polymerase II (RPB3) with transcription factor-4 (ATF4).FEBS Lett. 547: 15-19, 2003.2. Hai, T., Liu, F., Coukos, W. J., Green, M. R.Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.Genes Dev. 3: 2083-2090, 1989.3. Karpinski, B. A., Morle, G. D., Huggenvik, J., Uhler, M. D., Leiden, J. M.Molecular cloning of human CREB-2: an ATF/CREB transcription factor that can negatively regulate transcription from the cAMP response element.Proc. Nat. Acad. Sci. 89: 4820-4824, 1992.

Other Notes

Small volumes of anti-ATF4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATF4 purchase

MBS1758067 is an antibody targeting ATF4. Also known as Anti-ATF4 Antibody Picoband. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATF4. It may not necessarily be applicable to this product.

NCBI GI #
33469976
NCBI GeneID
468
NCBI Accession #
NP_001666.2
UniProt Primary Accession #
P18848
UniProt Secondary Accession #
Q9UH31
UniProt Related Accession #
P18848
GenBank Nucleotide #
NM_001675.2
NCBI Official Full Name
cyclic AMP-dependent transcription factor ATF-4
NCBI Official Synonym Full Names
activating transcription factor 4
NCBI Official Symbol
ATF4
NCBI Official Synonym Symbols
CREB2; TXREB; CREB-2; TAXREB67
UniProt Protein Name
Cyclic AMP-dependent transcription factor ATF-4
UniProt Synonym Protein Names
Activating transcription factor 4; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; Tax-responsive enhancer element-binding protein 67
Protein Family
Cyclic AMP-dependent transcription factor
UniProt Gene Name
ATF4
UniProt Synonym Gene Names
CREB2; TXREB; cAMP-dependent transcription factor ATF-4; CREB-2; cAMP-responsive element-binding protein 2; TaxREB67
UniProt Entry Name
ATF4_HUMAN
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-4; DNA-binding protein TAXREB67; tax-responsive enhancer element B67; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; cyclic AMP-r

NCBI Summary for ATF4

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011]
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UniProt Comments for ATF4

ATF-4: a transcription factor that is a member of the leucine zipper family. Isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). Involved in cisplatin resistance. Other members of the family include AP-1 transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; neuron projection; cytoplasm; microtubule organizing center; nucleus

Molecular Function: protein C-terminus binding; protein binding; leucine zipper domain binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; amino acid metabolic process; unfolded protein response; positive regulation of apoptosis; negative regulation of potassium ion transport; positive regulation of transcription, DNA-dependent; gluconeogenesis; positive regulation of transcription from RNA polymerase I promoter; cellular response to glucose starvation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; gamma-aminobutyric acid signaling pathway
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Research Articles on ATF4

1. ATF4 acts as a positive regulator in TLR4-triggered cytokine production.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ATF4 antibody

 Reference Product  PubMed Publications
 DDIT3 antibody  >188 publications with ATF4 and DDIT3
 JUN antibody  >110 publications with ATF4 and JUN
 TRIB3 antibody  >25 publications with ATF4 and TRIB3
 NFE2L2 antibody  >14 publications with ATF4 and NFE2L2
 DISC1 antibody  >8 publications with ATF4 and DISC1
 CEBPB antibody  >3 publications with ATF4 and CEBPB
 TTR antibody  >1 publications with ATF4 and TTR
 POLR2C antibody  >1 publications with ATF4 and POLR2C
 NFE2L1 antibody  >1 publications with ATF4 and NFE2L1
 CEBPG antibody  >1 publications with ATF4 and CEBPG

Pathways associated with anti-ATF4 antibody

 Products by Pathway  Pathway Diagram
 Activation Of Chaperone Genes By ATF6-alpha Pathway antibodies  Activation Of Chaperone Genes By ATF6-alpha Pathway Diagram
 Activation Of Chaperones By ATF6-alpha Pathway antibodies  Activation Of Chaperones By ATF6-alpha Pathway Diagram
 Activation Of Genes By ATF4 Pathway antibodies  Activation Of Genes By ATF4 Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Cholinergic Synapse Pathway antibodies  Cholinergic Synapse Pathway Diagram
 Cocaine Addiction Pathway antibodies  Cocaine Addiction Pathway Diagram
 Cocaine Addiction Pathway antibodies  Cocaine Addiction Pathway Diagram

Diseases associated with anti-ATF4 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >122 publications with ATF4 and Neoplasms
 Nervous System Diseases Antibodies  >53 publications with ATF4 and Nervous System Diseases
 Drug Toxicity Antibodies  >43 publications with ATF4 and Drug Toxicity
 Cardiovascular Diseases Antibodies  >42 publications with ATF4 and Cardiovascular Diseases
 Inflammation Antibodies  >39 publications with ATF4 and Inflammation
 Brain Diseases Antibodies  >35 publications with ATF4 and Brain Diseases
 Disease Models, Animal Antibodies  >31 publications with ATF4 and Disease Models, Animal
 Liver Diseases Antibodies  >28 publications with ATF4 and Liver Diseases
 Breast Neoplasms Antibodies  >20 publications with ATF4 and Breast Neoplasms
 Anoxia Antibodies  >18 publications with ATF4 and Anoxia

Organs/Tissues associated with anti-ATF4 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >92 publications with ATF4 and Brain
 Bone Antibodies  >80 publications with ATF4 and Bone
 Liver Antibodies  >61 publications with ATF4 and Liver
 Blood Antibodies  >57 publications with ATF4 and Blood
 Vascular Antibodies  >44 publications with ATF4 and Vascular
 Nerve Antibodies  >33 publications with ATF4 and Nerve
 Muscle Antibodies  >33 publications with ATF4 and Muscle
 Lung Antibodies  >28 publications with ATF4 and Lung
 Pancreas Antibodies  >28 publications with ATF4 and Pancreas
 Kidney Antibodies  >24 publications with ATF4 and Kidney
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