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BBS1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758363

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
BBS1, Polyclonal Antibody
Full Product Name
Anti-BBS1 Antibody Picoband
Product Gene Name
anti-BBS1 antibody
Product Synonym Names
Bardet-Biedl syndrome 1 protein; Bardet-Biedl syndrome 1; BBS1; BBS2L2; Bardet-Biedl syndrome 1 protein; BBS2-like protein 2
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
49,006 Da
OMIM
209900
3D Structure
ModBase 3D Structure for Q8NFJ9
Immunogen
E coli-derived human ATF5 recombinant protein (Position: L11-R275). Human ATF5 shares 86.5% and 87.2% amino acid (aa) sequence identity with mouse and rat ATF5, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins

Product Categories/Family for anti-BBS1 antibody

Primary Antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-BBS1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-BBS1 antibody
WB: 0.25-0.5ug/ml, Mouse, Rat
ELISA: 0.1-0.5ug/ml, Human

Western Blot (WB) of anti-BBS1 antibody

Figure 1. Western blot analysis of BBS1 using anti-BBS1 antibody (MBS1758363).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BBS1 antigen affinity purified polyclonal antibody (#MBS1758363) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for BBS1 at approximately 65 kDa. The expected band size for BBS1 is at 65 kDa.
Rabbit Anti-BBS1 Polyclonal Antibody – Cat# MBS1758363

Product References and Citations for anti-BBS1 antibody

1. Badano, J. L., Ansley, S. J., Leitch, C. C., Lewis, R. A., Lupski, J. R., Katsanis, N. Identification of a novel Bardet-Biedl syndrome protein, BBS7, that shares structural features with BBS1 and BBS2. Am. J. Hum. Genet. 72: 650-658, 2003.2. Badano, J. L., Kim, J. C., Hoskins, B. E., Lewis, R. A., Ansley, S. J., Cutler, D. J., Castellan, C., Beales, P. L., Leroux, M. R., Katsanis, N. Heterozygous mutations in BBS1, BBS2 and BBS6 have a potential epistatic effect on Bardet-Biedl patients with two mutations at a second BBS locus. Hum. Molec. Genet. 12: 1651-1659, 2003.3. Beales, P. L., Badano, J. L., Ross, A. J., Ansley, S. J., Hoskins, B. E., Kirsten, B., Mein, C. A., Froguel, P., Scambler, P. J., Lewis, R. A., Lupski, J. R., Katsanis, N. Genetic interaction of BBS1 mutations with alleles at other BBS loci can result in non-mendelian Bardet-Biedl syndrome. Am. J. Hum. Genet. 72: 1187-1199, 2003.

Other Notes

Small volumes of anti-BBS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BBS1 purchase

MBS1758363 is an antibody targeting BBS1. Also known as Anti-BBS1 Antibody Picoband. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BBS1. It may not necessarily be applicable to this product.

NCBI GI #
28395045
NCBI GeneID
582
NCBI Accession #
NP_078925.3
UniProt Primary Accession #
Q8NFJ9
UniProt Secondary Accession #
Q32MM9; Q32MN0; Q96SN4
UniProt Related Accession #
Q8NFJ9
GenBank Nucleotide #
NM_024649.4
NCBI Official Full Name
Bardet-Biedl syndrome 1 protein
NCBI Official Synonym Full Names
Bardet-Biedl syndrome 1
NCBI Official Symbol
BBS1
NCBI Official Synonym Symbols
BBS2L2
UniProt Protein Name
Bardet-Biedl syndrome 1 protein
UniProt Synonym Protein Names
BBS2-like protein 2
Protein Family
Bardet-Biedl syndrome 1 protein
UniProt Gene Name
BBS1
UniProt Synonym Gene Names
BBS2L2
NCBI Protein Information
Bardet-Biedl syndrome 1 protein

NCBI Summary for BBS1

Mutations in this gene have been observed in patients with the major form (type 1) of Bardet-Biedl syndrome. The encoded protein may play a role in eye, limb, cardiac and reproductive system development. [provided by RefSeq, Jul 2008]

UniProt Comments for BBS1

The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization (PubMed:17574030, PubMed:22072986). Plays a role in olfactory cilium biogenesis/maintenance and trafficking ().
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Research Articles on BBS1

1. Importantly, one Japanese and one Omani families carried compound biallelic mutations in two distinct genes (TMEM67/RPGRIP1L and TMEM138/BBS1, respectively).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BBS1 antibody

 Reference Product  PubMed Publications
 BBS2 antibody  >49 publications with BBS1 and BBS2
 BBS4 antibody  >47 publications with BBS1 and BBS4
 BBS7 antibody  >20 publications with BBS1 and BBS7
 BBS12 antibody  >17 publications with BBS1 and BBS12
 BBS5 antibody  >17 publications with BBS1 and BBS5
 BBS9 antibody  >6 publications with BBS1 and BBS9
 ARL6 antibody  >5 publications with BBS1 and ARL6
 TTC8 antibody  >4 publications with BBS1 and TTC8

Diseases associated with anti-BBS1 antibody

 Disease Name  Pubmed Publications
 Bardet-Biedl Syndrome Antibodies  >108 publications with BBS1 and Bardet-Biedl Syndrome
 Heart Defects, Congenital Antibodies  >6 publications with BBS1 and Heart Defects, Congenital
 Death Antibodies  >3 publications with BBS1 and Death
 Infertility, Male Antibodies  >3 publications with BBS1 and Infertility, Male
 Gonadal Disorders Antibodies  >3 publications with BBS1 and Gonadal Disorders
 Disease Progression Antibodies  >3 publications with BBS1 and Disease Progression
 Weight Gain Antibodies  >2 publications with BBS1 and Weight Gain
 Weight Loss Antibodies  >2 publications with BBS1 and Weight Loss
 Inflammation Antibodies  >2 publications with BBS1 and Inflammation
 Cognition Disorders Antibodies  >1 publications with BBS1 and Cognition Disorders

Organs/Tissues associated with anti-BBS1 antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >24 publications with BBS1 and Kidney
 Eye Antibodies  >24 publications with BBS1 and Eye
 Heart Antibodies  >14 publications with BBS1 and Heart
 Blood Antibodies  >6 publications with BBS1 and Blood
 Brain Antibodies  >6 publications with BBS1 and Brain
 Liver Antibodies  >5 publications with BBS1 and Liver
 Stomach Antibodies  >4 publications with BBS1 and Stomach
 Skin Antibodies  >2 publications with BBS1 and Skin
 Embryonic Tissue Antibodies  >2 publications with BBS1 and Embryonic Tissue
 Adipose Tissue Antibodies  >2 publications with BBS1 and Adipose Tissue
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