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BCLW Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9605063

Function: Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX.Subunit Structure: Interacts with HIF3A (via C-terminus domain). Interacts with… More details

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Product Overview

Online Datasheet in PDF
Product Name
BCLW, Polyclonal Antibody
Full Product Name
BCLW Antibody
Product Gene Name
anti-BCLW antibody
Product Synonym Gene Name
BCL2L2
Product Synonym Names
Apoptosis regulator BCL W; Apoptosis regulator Bcl-W; B2CL2_HUMAN; BCL 2 Like 2; Bcl 2 like 2 protein; Bcl 2L2; BCL W; Bcl-2-like protein 2; Bcl2 L2; BCL2 like 2; BCL2 like 2 protein; Bcl2-L-2; Bcl2l2; BCLW; KIAA0271; PPP1R51; Protein phosphatase 1 regulatory subunit 51
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
BCLW antibody detects endogenous levels of total BCLW
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
BCL2L2 peptide (MBS9623780) is used for blocking the activity of BCL2L2 antibody (MBS9605063)
Molecular Weight
Observed: 25 kD
Predicted: 21 kDa
3D Structure
ModBase 3D Structure for Q92843
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide
Subcellular Location
Mitochondrion Membrane. Loosely associated with the mitochondrial membrane in healthy cells. During apoptosis, tightly bound to the membrane.
Tissue Specificity
Expressed (at protein level) in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines.
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-BCLW antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-BCLW antibody
WB: 1:500-1:1000
IF/ICC: 1:100-1:500

Western Blot (WB) of anti-BCLW antibody

Western blot analysis of extracts from COLO cells, using BCLW antibody.
Rabbit Anti-BCLW Polyclonal Antibody – Cat# MBS9605063

Immunofluorescene (IF) of anti-BCLW antibody

MBS9605063 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Rabbit Anti-BCLW Polyclonal Antibody, Immunofluorescene (IF) – Cat# MBS9605063

Other Notes

Small volumes of anti-BCLW antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BCLW purchase

MBS9605063 is an antibody targeting BCLW. Also known as BCLW Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BCLW. It may not necessarily be applicable to this product.

NCBI GI #
315360668
NCBI GeneID
599
NCBI Accession #
NP_001186768.1
UniProt Primary Accession #
Q92843
UniProt Secondary Accession #
Q2M3U0; Q5U0H4; A8K0F4
UniProt Related Accession #
Q92843
GenBank Nucleotide #
NM_001199839.1
NCBI Official Full Name
bcl-2-like protein 2
NCBI Official Synonym Full Names
BCL2 like 2
NCBI Official Symbol
BCL2L2
NCBI Official Synonym Symbols
BCLW; BCL-W; PPP1R51; BCL2-L-2
UniProt Protein Name
Bcl-2-like protein 2
UniProt Synonym Protein Names
Apoptosis regulator Bcl-W
UniProt Gene Name
BCL2L2
UniProt Synonym Gene Names
BCLW; KIAA0271; Bcl2-L-2
NCBI Protein Information
bcl-2-like protein 2

NCBI Summary for BCLW

This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream PABPN1 (poly(A) binding protein, nuclear 1) gene. [provided by RefSeq, Dec 2010]
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UniProt Comments for BCLW

Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX.

Research Articles on BCLW

1. Our comprehensive analysis indicates B-cell lymphomas commonly select for BCLW overexpression in combination with or instead of other antiapoptotic BCL2 family members.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BCLW antibody

 Reference Product  PubMed Publications
 BAD antibody  >22 publications with BCLW and BAD
 BID antibody  >12 publications with BCLW and BID
 BMF antibody  >3 publications with BCLW and BMF
 BIK antibody  >3 publications with BCLW and BIK
 BBC3 antibody  >3 publications with BCLW and BBC3
 HRK antibody  >1 publications with BCLW and HRK

Pathways associated with anti-BCLW antibody

 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram

Diseases associated with anti-BCLW antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >50 publications with BCLW and Neoplasms
 Nervous System Diseases Antibodies  >14 publications with BCLW and Nervous System Diseases
 Lymphoma Antibodies  >12 publications with BCLW and Lymphoma
 Necrosis Antibodies  >11 publications with BCLW and Necrosis
 Drug Toxicity Antibodies  >9 publications with BCLW and Drug Toxicity
 Liver Diseases Antibodies  >8 publications with BCLW and Liver Diseases
 Liver Neoplasms Antibodies  >8 publications with BCLW and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >6 publications with BCLW and Carcinoma, Hepatocellular
 Lung Neoplasms Antibodies  >6 publications with BCLW and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >5 publications with BCLW and Neoplasms, Experimental

Organs/Tissues associated with anti-BCLW antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >24 publications with BCLW and Brain
 Testis Antibodies  >12 publications with BCLW and Testis
 Intestine Antibodies  >10 publications with BCLW and Intestine
 Liver Antibodies  >9 publications with BCLW and Liver
 Lung Antibodies  >8 publications with BCLW and Lung
 Embryonic Tissue Antibodies  >5 publications with BCLW and Embryonic Tissue
 Kidney Antibodies  >3 publications with BCLW and Kidney
 Muscle Antibodies  >3 publications with BCLW and Muscle
 Ovary Antibodies  >3 publications with BCLW and Ovary
 Skin Antibodies  >2 publications with BCLW and Skin
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