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cdc2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS623445

Entry of all eukaryotic cells into M-phase of the cell cycle is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161 (1-4). The critical regulatory step in… More details

$830 / 0.1 mL +1 FREE 8GB USB
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
cdc2, Polyclonal Antibody
Full Product Name
cdc2, phosphorylated (Thr161)
Product Gene Name
Product Synonym Names
Anti -cdc2, phosphorylated (Thr161)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Detects endogenous levels of cdc2 only when phosphorylated at Thr161. Crossreacts with endogenous cdk2 phosphorylated at Thr160. Species Crossreactivity: Mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Chromosome Location
Chromosome: 2L; NT_033779.4 (10384739..10386263, complement). Location: 31D11-31D11
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Thr161 of human cdc2 (KLH coupled).
Applications Tested/Suitable for anti-cdc2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-cdc2 antibody
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry and Immufluorescence.
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Immunocytochemistry (for IF): 1:200

Western Blot (WB) of anti-cdc2 antibody

Western blot analysis of lambda protein phosphatase (lambda-PPase) treated cdc2 kinase, using MBS623445
Rabbit Anti-cdc2 Polyclonal Antibody – Cat# MBS623445

Western Blot (WB) of anti-cdc2 antibody

Western blot analysis of HeLa samples immunoprecipitated with MBS623445and then probed with control cdc2 (POH1) Monoclonal Antibody #9116.
Rabbit Anti-cdc2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS623445

Western Blot (WB) of anti-cdc2 antibody

Western blot analysis of extracts from HeLa cells synchronized and enriched for different stages of the cell cycle, and probed with MBS623445(upper) or control cdc2 (POH1) Monoclonal Antibody #9116 (lower).
Rabbit Anti-cdc2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS623445

Immunofluorescence (IF) of anti-cdc2 antibody

Dual immunofluorescent staining of growing HeLa cells, using cdc2 (POH1) Monoclonal Antibody (left) or MBS623445(right).
Rabbit Anti-cdc2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS623445

Other Notes

Small volumes of anti-cdc2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for cdc2 purchase

MBS623445 is an antibody targeting cdc2. Also known as cdc2, phosphorylated (Thr161). Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for cdc2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
cdc2
NCBI Official Symbol
cdc2
NCBI Official Synonym Symbols
5363; cdc; Cdc2; CDC2; cdc2Dm; CDCDm; cdk1; Cdk1; CDK1; CDK1/CDC2; CG5363; Dcdc2; Dm cdc2; Dmcdc2; DmCdc2; DmCdk1; DmelCG5363; group 4; l(2)31Eh
NCBI Protein Information
CG5363 gene product from transcript CG5363-RA; cdc2; CG5363-PA; cdc2-PA; cyclin-dependent kinase 1; CDC2 kinase; cyclin dependent kinase

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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