Rabbit Anti-CISH Polyclonal Antibody – Cat# MBS9609231

Western blot analysis of CISH using Jurkat whole cell lysates

CISH Antibody – Rabbit Polyclonal

Name: CISH Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS9609231
Price: 325 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9609231

Function: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin… More details

$325 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CISH, Polyclonal Antibody

Full Product Name

CISH Antibody

Product Gene Name

anti-CISH antibody

Product Synonym Names

BACTS2; cis 1; CIS; CIS-1; CIS1; Cish; CISH_HUMAN; Cytokine inducible inhibitor of signaling type 1B; Cytokine inducible SH2 containing protein; Cytokine-inducible SH2-containing protein; G18; Protein G18; SOCS; Suppressor of cytokine signaling

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Purity/Purification

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Concentration

1mg/ml (lot specific)

Specificity

CISH antibody detects endogenous levels of total CISH

Preparation and Storage

Store at -20 degree C. Stable for 12 months from date of receipt.

Antibody/Peptide Pairs

CISH peptide (MBS9627935) is used for blocking the activity of CISH antibody (MBS9609231)

Molecular Weight

Observed: 29 kDa
Predicted: 29 kDa

3D Structure

ModBase 3D Structure for Q9NSE2

Supply Chain Verification

Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.

Immunogen

A synthesized peptide derived from human CISH

Tissue Specificity

Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.

Predicted Cross Reactivity

Bovine, Sheep, Rabbit, Dog

Similarity

Bovine (83%), Sheep (83%), Rabbit (92%), Dog (83%)

Conjugation

Unconjugated

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-CISH antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-CISH antibody

WB: 1:1000-3000

Other Notes

Small volumes of anti-CISH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CISH purchase

MBS9609231 is an antibody targeting CISH. Also known as CISH Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CISH. It may not necessarily be applicable to this product.

NCBI GI #

195976771

NCBI GeneID

1154

NCBI Accession #

NP_037456.5

UniProt Primary Accession #

Q9NSE2

UniProt Secondary Accession #

Q9NS38; Q9Y5R1; B2R9N1; G5E9R1

UniProt Related Accession #

Q9NSE2

GenBank Nucleotide #

NM_013324.5

NCBI Official Full Name

cytokine-inducible SH2-containing protein isoform 1

NCBI Official Synonym Full Names

cytokine inducible SH2 containing protein

NCBI Official Symbol

CISH

NCBI Official Synonym Symbols

CIS; G18; SOCS; CIS-1; BACTS2

UniProt Protein Name

Cytokine-inducible SH2-containing protein

UniProt Synonym Protein Names

CIS-1; Protein G18; Suppressor of cytokine signaling; SOCS

Protein Family

Cytokine-inducible SH2-containing protein

UniProt Gene Name

CISH

UniProt Synonym Gene Names

G18; CIS; SOCS

NCBI Protein Information

cytokine-inducible SH2-containing protein

NCBI Summary for CISH

The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

UniProt Comments for CISH

SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins ().

Research Articles on CISH

1. identified P2RX2, KCNQ5, ERBB3 and SOCS3 to be associated with the progression of age-related hearing impairment

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CISH antibody

Reference Product	PubMed Publications
SOCS2 antibody	>18 publications with CISH and SOCS2
JAK2 antibody	>9 publications with CISH and JAK2
STAT3 antibody	>4 publications with CISH and STAT3
IL2RA antibody	>1 publications with CISH and IL2RA
JAK3 antibody	>1 publications with CISH and JAK3

Pathways associated with anti-CISH antibody

Products by Pathway	Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
ECS Complex Pathway antibodies	ECS Complex Pathway Diagram
ECS Complex Pathway antibodies	ECS Complex Pathway Diagram
EPO Receptor Signaling Pathway antibodies	EPO Receptor Signaling Pathway Diagram
Growth Hormone Receptor Signaling Pathway antibodies	Growth Hormone Receptor Signaling Pathway Diagram
IL-2 Signaling Pathway antibodies	IL-2 Signaling Pathway Diagram
IL-3 Signaling Pathway antibodies	IL-3 Signaling Pathway Diagram
IL2-mediated Signaling Events Pathway antibodies	IL2-mediated Signaling Events Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
JAK-STAT Signaling Pathway antibodies	JAK-STAT Signaling Pathway Diagram

Diseases associated with anti-CISH antibody

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>162 publications with CISH and Breast Neoplasms
Adenocarcinoma Antibodies	>81 publications with CISH and Adenocarcinoma
Chromosome Aberrations Antibodies	>37 publications with CISH and Chromosome Aberrations
Lung Neoplasms Antibodies	>28 publications with CISH and Lung Neoplasms
Nervous System Diseases Antibodies	>22 publications with CISH and Nervous System Diseases
Inflammation Antibodies	>14 publications with CISH and Inflammation
Neoplasms, Experimental Antibodies	>14 publications with CISH and Neoplasms, Experimental
Liver Diseases Antibodies	>10 publications with CISH and Liver Diseases
Necrosis Antibodies	>9 publications with CISH and Necrosis
Kidney Diseases Antibodies	>6 publications with CISH and Kidney Diseases

Organs/Tissues associated with anti-CISH antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>51 publications with CISH and Blood
Lung Antibodies	>35 publications with CISH and Lung
Lymph Node Antibodies	>26 publications with CISH and Lymph Node
Brain Antibodies	>20 publications with CISH and Brain
Uterus Antibodies	>17 publications with CISH and Uterus
Skin Antibodies	>16 publications with CISH and Skin
Bone Antibodies	>10 publications with CISH and Bone
Kidney Antibodies	>9 publications with CISH and Kidney
Bone Marrow Antibodies	>6 publications with CISH and Bone Marrow
Intestine Antibodies	>4 publications with CISH and Intestine