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CIT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1760159

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
CIT, Polyclonal Antibody
Full Product Name
Anti-CIT Antibody Picoband
Product Gene Name
anti-CIT antibody
Product Synonym Names
Citron Rho-interacting kinase; citron rho-interacting serine/threonine kinase; CIT; CRIK; KIAA0949; STK21; Citron Rho-interacting kinase; CRIK; EC 2.7.11.1; Serine/threonine-protein kinase 21
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
231,431 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (120123595..120315095, complement). Location: 12q24
OMIM
605629
3D Structure
ModBase 3D Structure for O14578
Immunogen
E coli-derived human CIT recombinant protein (Position: Q470-H1505). Human CIT shares 95.2% and 94.7% amino acid (aa) sequence identity with mouse and rat CIT, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Cross Reactivity
No cross-reactivity with other proteins

Product Categories/Family for anti-CIT antibody

Primary Antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CIT antibody
Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-CIT antibody
WB: 0.25-0.5ug/ml, Human, Mouse, Rat
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-CIT antibody

Figure 1. Western blot analysis of CIT using anti-CIT antibody (MBS1760159).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CIT antigen affinity purified polyclonal antibody (MBS1760159) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for CIT at approximately 231 kDa. The expected band size for CIT is at 231 kDa.
Rabbit Anti-CIT Polyclonal Antibody – Cat# MBS1760159

Flow Cytometry (FC/FACS) of anti-CIT antibody

Figure 2. Flow Cytometry analysis of SiHa cells using anti-CIT antibody (MBS1760159).Overlay histogram showing SiHa cells stained with MBS1760159 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CIT Antibody (MBS1760159, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Rabbit Anti-CIT Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1760159

Product References and Citations for anti-CIT antibody

1. Basit, S., Al-Harbi, K. M., Alhijji, S. A. M., Albalawi, A. M., Alharby, E., Eldardear, A., Samman, M. I. CIT, a gene involved in neurogenic cytokinesis, is mutated in human primary microcephaly. Hum. Genet. 135: 1199-1207, 2016.2. Di Cunto, F., Calautti, E., Hsiao, J., Ong, L., Topley, G., Turco, E., Dotto, G. P. Citron Rho-interacting kinase, a novel tissue-specific ser/thr kinase encompassing the Rho-Rac-binding protein citron. J. Biol. Chem. 273: 29706-29711, 1998.3. Di Cunto, F., Imarisio, S., Hirsch, E., Broccoli, V., Bulfone, A., Migheli, A., Atzori, C., Turco, E., Triolo, R., Dotto, G. P., Silengo, L., Altruda, F. Defective neurogenesis in citron kinase knockout mice by altered cytokinesis and massive apoptosis. Neuron 28: 115-127, 2000.

Other Notes

Small volumes of anti-CIT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CIT purchase

MBS1760159 is an antibody targeting CIT. Also known as Anti-CIT Antibody Picoband. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CIT. It may not necessarily be applicable to this product.

NCBI GI #
332801082
NCBI GeneID
11113
NCBI Accession #
NP_001193928.1
UniProt Primary Accession #
O14578
UniProt Secondary Accession #
Q2M5E1; Q6XUH8; Q86UQ9; Q9UPZ7
UniProt Related Accession #
O14578
GenBank Nucleotide #
NM_001206999.1
NCBI Official Full Name
citron Rho-interacting kinase isoform 1
NCBI Official Synonym Full Names
citron (rho-interacting, serine/threonine kinase 21)
NCBI Official Symbol
CIT
NCBI Official Synonym Symbols
CRIK; STK21
UniProt Protein Name
Citron Rho-interacting kinase
UniProt Synonym Protein Names
Serine/threonine-protein kinase 21
Protein Family
Citron Rho-interacting kinase
UniProt Gene Name
CIT
UniProt Synonym Gene Names
CRIK; KIAA0949; STK21; CRIK
UniProt Entry Name
CTRO_HUMAN
NCBI Protein Information
citron Rho-interacting kinase; serine/threonine-protein kinase 21

NCBI Summary for CIT

This gene encodes a serine/threonine-protein kinase that functions in cell division. Together with the kinesin KIF14, this protein localizes to the central spindle and midbody, and functions to promote efficient cytokinesis. This protein is involved in central nervous system development. Polymorphisms in this gene are associated with bipolar disorder and risk for schizophrenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]
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UniProt Comments for CIT

CRIK: an AGC kinase that is a RHO/RAC effector. Binds to the GTP-bound forms of RHO and RAC1. Required for postnatal hippocampal neurogenesis. Rho/CRIK suppresses differentiation and is associated with induction of KyoT1/2, a LIM domain protein gene implicated in integrin-mediated processes and Notch signaling. Plays an important role in the regulation of cytokinesis and the development of the central nervous system. Overexpressed in mantle-cell lymphoma (MCL), an aggressive type of B-cell lymphoma. Three alternatively spliced isoforms have been described.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; DMPK family

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: ruffle; Golgi cisterna; cell soma; membrane; plasma membrane; vacuole; actin cytoskeleton

Molecular Function: protein serine/threonine kinase activity; protein binding; Rho GTPase binding; metal ion binding; SH3 domain binding; ATP binding; PDZ domain binding

Biological Process: positive regulation of cytokinesis; mitosis; mitotic sister chromatid segregation; generation of neurons; regulation of catalytic activity; regulation of actin polymerization and/or depolymerization; dendrite development; cytokinesis; spermatogenesis; negative regulation of dendrite morphogenesis; G2/M transition of mitotic cell cycle; Golgi organization and biogenesis; protein amino acid phosphorylation; mitotic metaphase/anaphase transition
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Research Articles on CIT

1. High CIT expression is associated with prostate cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CIT antibody

 Reference Product  PubMed Publications
 RHOA antibody  >4 publications with CIT and RHOA
 DISC1 antibody  >3 publications with CIT and DISC1

Pathways associated with anti-CIT antibody

 Products by Pathway  Pathway Diagram
 G13 Signaling Pathway antibodies  G13 Signaling Pathway Diagram

Diseases associated with anti-CIT antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >179 publications with CIT and Neoplasms
 Immune System Diseases Antibodies  >149 publications with CIT and Immune System Diseases
 Cardiovascular Diseases Antibodies  >140 publications with CIT and Cardiovascular Diseases
 Drug Toxicity Antibodies  >113 publications with CIT and Drug Toxicity
 Kidney Diseases Antibodies  >76 publications with CIT and Kidney Diseases
 Liver Diseases Antibodies  >66 publications with CIT and Liver Diseases
 Nerve Degeneration Antibodies  >56 publications with CIT and Nerve Degeneration
 Carcinoma Antibodies  >49 publications with CIT and Carcinoma
 Inflammation Antibodies  >42 publications with CIT and Inflammation
 Necrosis Antibodies  >41 publications with CIT and Necrosis

Organs/Tissues associated with anti-CIT antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >796 publications with CIT and Brain
 Blood Antibodies  >620 publications with CIT and Blood
 Kidney Antibodies  >244 publications with CIT and Kidney
 Liver Antibodies  >191 publications with CIT and Liver
 Heart Antibodies  >105 publications with CIT and Heart
 Muscle Antibodies  >78 publications with CIT and Muscle
 Skin Antibodies  >66 publications with CIT and Skin
 Intestine Antibodies  >63 publications with CIT and Intestine
 Lung Antibodies  >58 publications with CIT and Lung
 Bone Antibodies  >57 publications with CIT and Bone
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