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DAGLA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9612268

MBS9612268 is an antibody targeting DAGLA. Also known as DAGLA Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DAGLA, Polyclonal Antibody
Full Product Name
DAGLA Antibody
Product Gene Name
anti-DAGLA antibody
Product Synonym Gene Name
C11orf11, KIAA0659, NSDDR
Product Synonym Names
C11orf11; Chromosome 11 open reading frame 11; DAGL(ALPHA); Dagla; DAGLALPHA; DGL alpha; DGL-alpha; DGLA_HUMAN; Diacylglycerol lipase alpha; KIAA0659; Neural stem cell derived dendrite regulator; Neural stem cell-derived dendrite regulator; NSDDR; Sn1-specific diacylglycerol lipase alpha
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Concentration
~1mg/ml (lot specific)
Preparation and Storage
Store at -20 degrees C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
DAGLA peptide (MBS9622212) is used for blocking the activity of DAGLA antibody (MBS9612268)
Molecular Weight
114kDa
OMIM
phenotype 614015
3D Structure
ModBase 3D Structure for Q9Y4D2
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human DAGLA, corresponding to a region within C-terminal amino acids.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-DAGLA antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-DAGLA antibody
IF/ICC: 1:100-1:500
WB: 1:500-1:2000
IHC: 1:50-1:200
The optimal dilutions should be determined by the end user
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk, 1X TBS, 0.1% Tween20 at 4 degrees C with gentle shaking overnight.

Western Blot (WB) of anti-DAGLA antibody

Western blot analysis of extracts from NCI-H292, using DAGLA Antibody. The lane on the left was treated with blocking peptide.
Rabbit Anti-DAGLA Polyclonal Antibody – Cat# MBS9612268

Immunohistochemistry (IHC)-Paraffin of anti-DAGLA antibody

MBS9612268 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degrees C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-DAGLA Polyclonal Antibody, Immunohistochemistry (IHC)-Paraffin – Cat# MBS9612268

Immunofluorescence (IF)/Immunocytochemistry (ICC) of anti-DAGLA antibody

MBS9612268 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degrees C. Samples were then incubated with primary Ab(MBS9612268 1:200) and mouse anti-beta tubulin Ab(MBS9610328 1:200) for 1 hour at 37 degrees C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.
The nuclear counter stain is DAPI(blue).
Rabbit Anti-DAGLA Polyclonal Antibody, Immunofluorescence (IF)/Immunocytochemistry – Cat# MBS9612268

Other Notes

Small volumes of anti-DAGLA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DAGLA. It may not necessarily be applicable to this product.

NCBI GI #
27262632
NCBI GeneID
747
NCBI Accession #
NP_006124.1
UniProt Primary Accession #
Q9Y4D2
UniProt Related Accession #
Q9Y4D2
GenBank Nucleotide #
NP_006124.1
NCBI Official Full Name
sn1-specific diacylglycerol lipase alpha
NCBI Official Synonym Full Names
diacylglycerol lipase alpha
NCBI Official Symbol
DAGLA
NCBI Official Synonym Symbols
NSDDR; C11orf11; DAGLALPHA; DAGL(ALPHA)
UniProt Protein Name
Sn1-specific diacylglycerol lipase alpha
UniProt Synonym Protein Names
Neural stem cell-derived dendrite regulator
Protein Family
Sn1-specific diacylglycerol lipase
UniProt Gene Name
DAGLA
UniProt Synonym Gene Names
C11orf11; KIAA0659; NSDDR; DGL-alpha
UniProt Entry Name
DGLA_HUMAN
NCBI Protein Information
sn1-specific diacylglycerol lipase alpha

NCBI Summary for DAGLA

This gene encodes a diacylglycerol lipase. The encoded enzyme is involved in the biosynthesis of the endocannabinoid 2-arachidonoyl-glycerol.[provided by RefSeq, Nov 2010]

UniProt Comments for DAGLA

NSDDR: Catalyzes the hydrolysis of diacylglycerol (DAG) to 2- arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses. Defects in DAGLA may be a cause of spinocerebellar ataxia type 20 (SCA20). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA20 is an autosomal dominant, adult- onset form characterized by dysarthria due to spasmodic dysphonia followed by slowly progressive ataxia. A copy number variation consisting of a 260-kb duplication at chromosome 11q12.2-12.3 is responsible for SCA20. The critical gene within the duplicated segment may be DAGLA. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: Membrane protein, integral; EC 3.1.1.-; Membrane protein, multi-pass; Hydrolase

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: plasma membrane; integral to membrane

Molecular Function: metal ion binding; lipase activity

Biological Process: platelet activation; neuroblast proliferation; arachidonic acid metabolic process; diacylglycerol catabolic process; blood coagulation; neurotransmitter biosynthetic process
Show more

Research Articles on DAGLA

1. Our findings indicated the involvement of DAGLA in alcoholism, possibly by its genetic dysfunction and also by the influence of stress

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DAGLA antibody

 Reference Product  PubMed Publications
 FAAH antibody  >2 publications with DAGLA and FAAH
 CNR1 antibody  >1 publications with DAGLA and CNR1

Pathways associated with anti-DAGLA antibody

 Products by Pathway  Pathway Diagram
 Arachidonate Production From DAG Pathway antibodies  Arachidonate Production From DAG Pathway Diagram
 Effects Of PIP2 Hydrolysis Pathway antibodies  Effects Of PIP2 Hydrolysis Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 N-cadherin Signaling Events Pathway antibodies  N-cadherin Signaling Events Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Retrograde Endocannabinoid Signaling Pathway antibodies  Retrograde Endocannabinoid Signaling Pathway Diagram
 Retrograde Endocannabinoid Signaling Pathway antibodies  Retrograde Endocannabinoid Signaling Pathway Diagram

Diseases associated with anti-DAGLA antibody

 Disease Name  Pubmed Publications
 Central Nervous System Diseases Antibodies  >1 publications with DAGLA and Central Nervous System Diseases
 Disease Models, Animal Antibodies  >1 publications with DAGLA and Disease Models, Animal

Organs/Tissues associated with anti-DAGLA antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >2 publications with DAGLA and Brain
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