Rabbit Anti-DGCR8 Polyclonal Antibody – Cat# MBS9127160

Western blot analysis of extracts of various cell lines, using DGCR8 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

DGCR8 Antibody – Rabbit Polyclonal

Name: DGCR8 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS9127160
Price: 440 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9127160

This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

DGCR8, Polyclonal Antibody

Full Product Name

DGCR8 Polyclonal Antibody

Product Gene Name

anti-DGCR8 antibody

Product Synonym Names

Gy1; pasha; DGCRK6; C22orf12

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Purity/Purification

Affinity Purification

Concentration

1mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

773

OMIM

609030

3D Structure

ModBase 3D Structure for Q8WYQ5

Species

Human

Route

Synthetic Peptide

Immunogen

A synthetic peptide of human DGCR8

Calculated Molecular Weight

86kDa

Product Categories/Family for anti-DGCR8 antibody

Polyclonal

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-DGCR8 antibody

Western Blot (WB), Immunoprecipitation (IP)

Application Notes for anti-DGCR8 antibody

WB: 1:500 - 1:1000
IP: 1:20 - 1:50

Other Notes

Small volumes of anti-DGCR8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DGCR8 purchase

MBS9127160 is an antibody targeting DGCR8. Also known as DGCR8 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DGCR8. It may not necessarily be applicable to this product.

NCBI GI #

23813990

NCBI GeneID

54487

NCBI Accession #

Q8WYQ5.1

UniProt Primary Accession #

Q8WYQ5

UniProt Secondary Accession #

Q6DCB2; Q6MZE9; Q6Y2L0; Q96G39; Q96GP8; Q9H6L8; Q9H6T7; Q9NRW2; B2R8G1

UniProt Related Accession #

Q8WYQ5

NCBI Official Full Name

Microprocessor complex subunit DGCR8

NCBI Official Synonym Full Names

DGCR8 microprocessor complex subunit

NCBI Official Symbol

DGCR8

NCBI Official Synonym Symbols

Gy1; pasha; DGCRK6; C22orf12

UniProt Protein Name

Microprocessor complex subunit DGCR8

UniProt Synonym Protein Names

DiGeorge syndrome critical region 8

Protein Family

Microprocessor complex

UniProt Gene Name

DGCR8

UniProt Synonym Gene Names

C22orf12; DGCRK6

UniProt Entry Name

DGCR8_HUMAN

NCBI Protein Information

microprocessor complex subunit DGCR8; DiGeorge syndrome critical region 8; DiGeorge syndrome critical region gene 8

UniProt Comments for DGCR8

DGCR8: a double strand RNA binding protein (dsRBP) which is essential for the first processing step of primary miRNAs (pri-miRNAs). Apparently binds and is activated by heme. A nuclear complex of Drosha with DGCR8 cleaves pri-miRNA substrates into 60-70 nucleotide intermediates (pre-miRNA), thus initiating the microRNA maturation process. Drosha provides the catalytic site for this cleavage, whereas DGCR8 (Pasha) anchors substrate pri-miRNAs. Pre-miRNAs are then processed by the cytoplasmic Dicer-TRBP complex to approximately 20-22 nt mature miRNAs.

Protein type: Nucleolus; RNA processing; RNA-binding

Chromosomal Location of Human Ortholog: 22q11.2

Cellular Component: microtubule cytoskeleton; nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; protein homodimerization activity; ribonuclease III activity; metal ion binding; double-stranded RNA binding

Biological Process: gene expression; primary microRNA processing

Disease: Velocardiofacial Syndrome

Research Articles on DGCR8

1. multisite phosphorylation regulates DGCR8 protein stability, thereby raising microprocessor complex levels, changing the mature miRNA profile of the cell, and increasing cell proliferation and migration

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-DGCR8 antibody

Products by Pathway	Pathway Diagram
Direct P53 Effectors Pathway antibodies	Direct P53 Effectors Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
MicroRNA (miRNA) Biogenesis Pathway antibodies	MicroRNA (miRNA) Biogenesis Pathway Diagram
Regulatory RNA Pathways antibodies	Regulatory RNA Pathways Diagram

Diseases associated with anti-DGCR8 antibody

Disease Name	Pubmed Publications
Heart Diseases Antibodies	>12 publications with DGCR8 and Heart Diseases
Mental Disorders Antibodies	>12 publications with DGCR8 and Mental Disorders
Congenital Abnormalities Antibodies	>11 publications with DGCR8 and Congenital Abnormalities
Nervous System Diseases Antibodies	>10 publications with DGCR8 and Nervous System Diseases
Adenocarcinoma Antibodies	>9 publications with DGCR8 and Adenocarcinoma
Liver Diseases Antibodies	>7 publications with DGCR8 and Liver Diseases
Liver Neoplasms Antibodies	>4 publications with DGCR8 and Liver Neoplasms
Inflammation Antibodies	>4 publications with DGCR8 and Inflammation
Neoplasms, Experimental Antibodies	>4 publications with DGCR8 and Neoplasms, Experimental
Breast Neoplasms Antibodies	>4 publications with DGCR8 and Breast Neoplasms

Organs/Tissues associated with anti-DGCR8 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>33 publications with DGCR8 and Brain
Blood Antibodies	>16 publications with DGCR8 and Blood
Liver Antibodies	>13 publications with DGCR8 and Liver
Embryonic Tissue Antibodies	>11 publications with DGCR8 and Embryonic Tissue
Muscle Antibodies	>10 publications with DGCR8 and Muscle
Kidney Antibodies	>8 publications with DGCR8 and Kidney
Bone Antibodies	>7 publications with DGCR8 and Bone
Skin Antibodies	>6 publications with DGCR8 and Skin
Lung Antibodies	>5 publications with DGCR8 and Lung
Thymus Antibodies	>3 publications with DGCR8 and Thymus