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DOK1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9608095

Description: The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Two… More details

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Product Overview

Online Datasheet in PDF
Product Name
DOK1, Polyclonal Antibody
Full Product Name
DOK1 Antibody
Product Gene Name
anti-DOK1 antibody
Product Synonym Names
Docking protein 1 62kD; Docking protein 1; DOK 1; DOK1; DOK1_HUMAN; Downstream of tyrosine kinase 1; p62(dok); P62DOK; pp62
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
DOK1 antibody detects endogenous levels of total DOK1
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
p62 Dok peptide (MBS9626807) is used for blocking the activity of p62 Dok antibody (MBS9608095)
Molecular Weight
Observed: 52 kDa
Predicted: 53 kDa
3D Structure
ModBase 3D Structure for Q99704
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human DOK1
Subcellular Location
Cytoplasm and Cytoplasm > Perinuclear Region.
Tissue Specificity
Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer.
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-DOK1 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-DOK1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500

Immunohistochemistry (IHC) of anti-DOK1 antibody

MBS9608095 at 1/100 staining Mouse pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-DOK1 Polyclonal Antibody – Cat# MBS9608095

Western Blot (WB) of anti-DOK1 antibody

Western blot analysis of Jurkat whole cell lysates, using DOK1 Antibody. The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-DOK1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9608095

Immunofluorescence (IF) of anti-DOK1 antibody

MBS9608095 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Rabbit Anti-DOK1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9608095

Other Notes

Small volumes of anti-DOK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DOK1 purchase

MBS9608095 is an antibody targeting DOK1. Also known as DOK1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DOK1. It may not necessarily be applicable to this product.

NCBI GI #
308737010
NCBI GeneID
1796
NCBI Accession #
NP_001184189.1
UniProt Primary Accession #
Q99704
UniProt Secondary Accession #
O43204; Q53TY2; Q9UHG6
UniProt Related Accession #
Q99704
GenBank Nucleotide #
NM_001197260.1
NCBI Official Full Name
docking protein 1 isoform b
NCBI Official Synonym Full Names
docking protein 1
NCBI Official Symbol
DOK1
NCBI Official Synonym Symbols
pp62; P62DOK
UniProt Protein Name
Docking protein 1
UniProt Synonym Protein Names
Downstream of tyrosine kinase 1; p62(dok); pp62
Protein Family
Docking protein
UniProt Gene Name
DOK1
NCBI Protein Information
docking protein 1

NCBI Summary for DOK1

The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]
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UniProt Comments for DOK1

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
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Research Articles on DOK1

1. Taken together, these results indicate that ATRA-enhanced expression of DOK1 activates PPARgamma leading to inhibition of cell proliferation and enhancement of cell apoptosis in MCF-7 cell.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DOK1 antibody

 Reference Product  PubMed Publications
 SRC antibody  >56 publications with DOK1 and SRC
 CSK antibody  >8 publications with DOK1 and CSK
 RET antibody  >7 publications with DOK1 and RET
 LCK antibody  >6 publications with DOK1 and LCK
 CRKL antibody  >6 publications with DOK1 and CRKL
 TEC antibody  >5 publications with DOK1 and TEC
 FYN antibody  >4 publications with DOK1 and FYN
 SH2D1A antibody  >4 publications with DOK1 and SH2D1A
 LYN antibody  >3 publications with DOK1 and LYN
 KIT antibody  >3 publications with DOK1 and KIT

Pathways associated with anti-DOK1 antibody

 Products by Pathway  Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 BCR Signaling Pathway antibodies  BCR Signaling Pathway Diagram
 Fc-epsilon Receptor I Signaling In Mast Cells Pathway antibodies  Fc-epsilon Receptor I Signaling In Mast Cells Pathway Diagram
 IGF1R Signaling Cascade Pathway antibodies  IGF1R Signaling Cascade Pathway Diagram
 IRS Activation Pathway antibodies  IRS Activation Pathway Diagram
 IRS-mediated Signalling Pathway antibodies  IRS-mediated Signalling Pathway Diagram
 IRS-related Events Pathway antibodies  IRS-related Events Pathway Diagram
 IRS-related Events Triggered By IGF1R Pathway antibodies  IRS-related Events Triggered By IGF1R Pathway Diagram
 Insulin Pathway antibodies  Insulin Pathway Diagram
 Insulin Receptor Signalling Cascade Pathway antibodies  Insulin Receptor Signalling Cascade Pathway Diagram

Diseases associated with anti-DOK1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >36 publications with DOK1 and Neoplasms
 Neoplasms, Experimental Antibodies  >7 publications with DOK1 and Neoplasms, Experimental
 Lymphoma Antibodies  >6 publications with DOK1 and Lymphoma
 Carcinoma Antibodies  >5 publications with DOK1 and Carcinoma
 Necrosis Antibodies  >4 publications with DOK1 and Necrosis
 Adenocarcinoma Antibodies  >3 publications with DOK1 and Adenocarcinoma
 Congenital Abnormalities Antibodies  >2 publications with DOK1 and Congenital Abnormalities
 Inflammation Antibodies  >2 publications with DOK1 and Inflammation
 Hyperplasia Antibodies  >2 publications with DOK1 and Hyperplasia
 Stomach Neoplasms Antibodies  >2 publications with DOK1 and Stomach Neoplasms

Organs/Tissues associated with anti-DOK1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >41 publications with DOK1 and Blood
 Bone Antibodies  >15 publications with DOK1 and Bone
 Bone Marrow Antibodies  >14 publications with DOK1 and Bone Marrow
 Lung Antibodies  >7 publications with DOK1 and Lung
 Muscle Antibodies  >6 publications with DOK1 and Muscle
 Embryonic Tissue Antibodies  >4 publications with DOK1 and Embryonic Tissue
 Adipose Tissue Antibodies  >3 publications with DOK1 and Adipose Tissue
 Connective Tissue Antibodies  >3 publications with DOK1 and Connective Tissue
 Spleen Antibodies  >3 publications with DOK1 and Spleen
 Heart Antibodies  >3 publications with DOK1 and Heart
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