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EGF Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9512477

This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important… More details

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Product Overview

Online Datasheet in PDF
Product Name
EGF, Polyclonal Antibody
Full Product Name
EGF Rabbit Polyclonal Antibody
Product Gene Name
anti-EGF antibody
Product Synonym Names
Pro-epidermal growth factor (EGF) [Cleaved into: Epidermal growth factor (Urogastrone)]; BH6842; Pro-epidermal growth factor (EGF) [Cleaved into: Epidermal growth factor (Urogastrone)]
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat, Mouse
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1mg/ml (lot specific)
Specificity
The antibody detects endogenous EGF
Preparation and Storage
Store at -20 degree C/1 year
Molecular Weight
1207
OMIM
gene 611718
3D Structure
ModBase 3D Structure for P01133
Immunogen
Synthetic peptide from human protein at AA range: 1000-1060
Modification
Unmodified/Total
Applications Tested/Suitable for anti-EGF antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)
Application Notes for anti-EGF antibody
WB: 1:500-2000
IHC-p: 1:500-200
ELISA: 1:10000-20000

Immunohistochemistry (IHC) of anti-EGF antibody

Immunohistochemical analysis of paraffin-embedded Human gastric cancer. 1, Antibody was diluted at 1:100(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Rabbit Anti-EGF Polyclonal Antibody – Cat# MBS9512477

Immunohistochemistry (IHC) of anti-EGF antibody

Immunohistochemical analysis of paraffin-embedded Human gastric cancer. 1, Antibody was diluted at 1:100(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Rabbit Anti-EGF Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9512477

Immunohistochemistry (IHC) of anti-EGF antibody

Immunohistochemical analysis of paraffin-embedded Human gastric cancer. 1, Antibody was diluted at 1:100(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Rabbit Anti-EGF Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9512477

Immunohistochemistry (IHC) of anti-EGF antibody

Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Rabbit Anti-EGF Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9512477

Immunohistochemistry (IHC) of anti-EGF antibody

Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Rabbit Anti-EGF Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9512477

Immunohistochemistry (IHC) of anti-EGF antibody

Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Rabbit Anti-EGF Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9512477

Immunohistochemistry (IHC) of anti-EGF antibody

Immunohistochemical analysis of paraffin-embedded human-stomach-cancer, antibody was diluted at 1:200
Rabbit Anti-EGF Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9512477

Immunohistochemistry (IHC) of anti-EGF antibody

Immunohistochemical analysis of paraffin-embedded human-kidney-cancer, antibody was diluted at 1:200
Rabbit Anti-EGF Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9512477

Immunohistochemistry (IHC) of anti-EGF antibody

Immunohistochemical analysis of paraffin-embedded human-kidney-cancer, antibody was diluted at 1:200
Rabbit Anti-EGF Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9512477

Western Blot (WB) of anti-EGF antibody

Western blot analysis of 293T mouse-kidney lysate, antibody was diluted at 2000. Secondary antibody was diluted at 1:20000
Rabbit Anti-EGF Polyclonal Antibody, Western Blot (WB) – Cat# MBS9512477

Other Notes

Small volumes of anti-EGF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EGF purchase

MBS9512477 is an antibody targeting EGF. Also known as EGF Rabbit Polyclonal Antibody. Reactive in Human, Rat, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EGF. It may not necessarily be applicable to this product.

NCBI GI #
251757262
NCBI GeneID
1950
NCBI Accession #
P01133.2
UniProt Primary Accession #
P01133
UniProt Secondary Accession #
Q52LZ6; B4DRK7; E7EVD2; E9PBF0
UniProt Related Accession #
P01133
NCBI Official Full Name
Pro-epidermal growth factor
NCBI Official Synonym Full Names
epidermal growth factor
NCBI Official Symbol
EGF
NCBI Official Synonym Symbols
URG; HOMG4
UniProt Protein Name
Pro-epidermal growth factor
UniProt Synonym Protein Names
Urogastrone
Protein Family
Pro-epidermal growth factor
UniProt Gene Name
EGF
UniProt Synonym Gene Names
EGF
UniProt Entry Name
EGF_HUMAN
NCBI Protein Information
pro-epidermal growth factor; beta-urogastrone

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EGF antibody

 Reference Product  PubMed Publications
 EGFR antibody  >5653 publications with EGF and EGFR
 ERBB2 antibody  >1352 publications with EGF and ERBB2
 SRC antibody  >1234 publications with EGF and SRC
 ERBB4 antibody  >406 publications with EGF and ERBB4
 GRB2 antibody  >362 publications with EGF and GRB2
 STAT3 antibody  >295 publications with EGF and STAT3
 ERBB3 antibody  >275 publications with EGF and ERBB3
 ADAM17 antibody  >115 publications with EGF and ADAM17
 FN1 antibody  >8 publications with EGF and FN1

Pathways associated with anti-EGF antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Arf6 Signaling Events Pathway antibodies  Arf6 Signaling Events Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Ceramide Signaling Pathway antibodies  Ceramide Signaling Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 Constitutive Signaling By EGFRvIII Pathway antibodies  Constitutive Signaling By EGFRvIII Pathway Diagram
 Constitutive Signaling By Ligand-Responsive EGFR Cancer Variants Pathway antibodies  Constitutive Signaling By Ligand-Responsive EGFR Cancer Variants Pathway Diagram

Diseases associated with anti-EGF antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >1679 publications with EGF and Breast Neoplasms
 Adenocarcinoma Antibodies  >1324 publications with EGF and Adenocarcinoma
 Necrosis Antibodies  >1005 publications with EGF and Necrosis
 Neoplasms, Experimental Antibodies  >891 publications with EGF and Neoplasms, Experimental
 Cell Transformation, Neoplastic Antibodies  >875 publications with EGF and Cell Transformation, Neoplastic
 Lung Neoplasms Antibodies  >850 publications with EGF and Lung Neoplasms
 Inflammation Antibodies  >734 publications with EGF and Inflammation
 Neoplasm Metastasis Antibodies  >641 publications with EGF and Neoplasm Metastasis
 Liver Diseases Antibodies  >574 publications with EGF and Liver Diseases
 Neoplasm Invasiveness Antibodies  >554 publications with EGF and Neoplasm Invasiveness

Organs/Tissues associated with anti-EGF antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1875 publications with EGF and Brain
 Lung Antibodies  >1810 publications with EGF and Lung
 Kidney Antibodies  >1615 publications with EGF and Kidney
 Skin Antibodies  >1429 publications with EGF and Skin
 Muscle Antibodies  >1343 publications with EGF and Muscle
 Embryonic Tissue Antibodies  >1128 publications with EGF and Embryonic Tissue
 Eye Antibodies  >927 publications with EGF and Eye
 Intestine Antibodies  >909 publications with EGF and Intestine
 Mouth Antibodies  >872 publications with EGF and Mouth
 Placenta Antibodies  >468 publications with EGF and Placenta
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