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EIF3E Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750421

Description: Eukaryotic translation initiation factor 3 subunit E is a protein that in humans is encoded by the EIF3E gene. The human homolog of EIF3E is located on chromosome region 8q22-q23. It is composed of 13 exons that span 45 kb of genomic DNA. EIF3E… More details

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Product Overview

Online Datasheet in PDF
Product Name
EIF3e, Polyclonal Antibody
Full Product Name
Anti-EIF3e Picoband Antibody
Product Gene Name
anti-EIF3E antibody
Product Synonym Names
Eukaryotic translation initiation factor 3 subunit E; eIF3e; Eukaryotic translation initiation factor 3 subunit 6; Viral integration site protein INT-6 homolog; eIF-3 p48; EIF3E; EIF3S6; INT6
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified
Concentration
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. (lot specific)
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
52221 MW
OMIM
U54562 mRNA
3D Structure
ModBase 3D Structure for P60228
Immunogen
E Coli-derived human EIF3e recombinant protein (Position: A160-Q241). Human EIF3e shares 100% amino acid (aa) sequence identity with both mouse and rat EIF3e.
Subcellular Localization
Cytoplasm. Nucleus, PML body.
Tissue Specificity
Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Product Categories/Family for anti-EIF3E antibody

Epigenetics and Nuclear Signaling; DNA/RNA; Translation; Ribosome
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-EIF3E antibody
Western Blot (WB)
Application Notes for anti-EIF3E antibody
WB: 0.1-0.5mug/ml

Western Blot (WB) of anti-EIF3E antibody

Figure 1. Western blot analysis of EIF3e using anti- EIF3e antibody (MBS1750421).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: mouse spleen tissue lysates,
Lane 4: COLO320 whole cell lysates,
Lane 5: HELA whole Cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- EIF3e antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EIF3e at approximately 52KD. The expected band size for EIF3e is at 52KD.
Rabbit Anti-EIF3E Polyclonal Antibody – Cat# MBS1750421

Other Notes

Small volumes of anti-EIF3E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EIF3E purchase

MBS1750421 is an antibody targeting EIF3E. Also known as Anti-EIF3e Picoband Antibody. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF3E. It may not necessarily be applicable to this product.

NCBI GI #
4503521
NCBI GeneID
3646
NCBI Accession #
NP_001559.1
UniProt Primary Accession #
P60228
UniProt Secondary Accession #
O43902; Q64058; Q64059; Q64252; Q6FG33; Q8WVK4
UniProt Related Accession #
P60228
GenBank Nucleotide #
NM_001568.2
NCBI Official Full Name
eukaryotic translation initiation factor 3 subunit E
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 3 subunit E
NCBI Official Symbol
EIF3E
NCBI Official Synonym Symbols
INT6; EIF3S6; EIF3-P48; eIF3-p46
UniProt Protein Name
Eukaryotic translation initiation factor 3 subunit E
UniProt Synonym Protein Names
Eukaryotic translation initiation factor 3 subunit 6
Protein Family
Eukaryotic translation initiation factor
UniProt Gene Name
EIF3E
UniProt Synonym Gene Names
eIF3e
NCBI Protein Information
eukaryotic translation initiation factor 3 subunit E

UniProt Comments for EIF3E

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway (PubMed:17468741). May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins (PubMed:17310990, PubMed:17324924).
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Research Articles on EIF3E

1. A transcript-specific eIF3 complex mediates global translational control of energy metabolism.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EIF3E antibody

 Reference Product  PubMed Publications
 EIF3A antibody  >71 publications with EIF3E and EIF3A
 EIF3I antibody  >35 publications with EIF3E and EIF3I
 EIF3B antibody  >28 publications with EIF3E and EIF3B
 EIF3F antibody  >27 publications with EIF3E and EIF3F
 EIF3H antibody  >25 publications with EIF3E and EIF3H
 EIF3C antibody  >23 publications with EIF3E and EIF3C
 EIF3M antibody  >10 publications with EIF3E and EIF3M
 EIF3D antibody  >7 publications with EIF3E and EIF3D
 EIF3L antibody  >7 publications with EIF3E and EIF3L

Pathways associated with anti-EIF3E antibody

 Products by Pathway  Pathway Diagram
 Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway antibodies  Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway Diagram
 Cap-dependent Translation Initiation Pathway antibodies  Cap-dependent Translation Initiation Pathway Diagram
 Eukaryotic Translation Initiation Pathway antibodies  Eukaryotic Translation Initiation Pathway Diagram
 Formation Of A Pool Of Free 40S Subunits Pathway antibodies  Formation Of A Pool Of Free 40S Subunits Pathway Diagram
 Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway antibodies  Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway Diagram
 GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway antibodies  GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 HIF-2-alpha Transcription Factor Network Pathway antibodies  HIF-2-alpha Transcription Factor Network Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram

Diseases associated with anti-EIF3E antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >19 publications with EIF3E and Nervous System Diseases
 Disease Models, Animal Antibodies  >11 publications with EIF3E and Disease Models, Animal
 Necrosis Antibodies  >11 publications with EIF3E and Necrosis
 Carcinoma, Hepatocellular Antibodies  >10 publications with EIF3E and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >10 publications with EIF3E and Liver Neoplasms
 Hyperplasia Antibodies  >6 publications with EIF3E and Hyperplasia
 Heart Diseases Antibodies  >6 publications with EIF3E and Heart Diseases
 Hypertrophy Antibodies  >6 publications with EIF3E and Hypertrophy
 Fibrosis Antibodies  >4 publications with EIF3E and Fibrosis
 Kidney Diseases Antibodies  >3 publications with EIF3E and Kidney Diseases

Organs/Tissues associated with anti-EIF3E antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >56 publications with EIF3E and Blood
 Liver Antibodies  >38 publications with EIF3E and Liver
 Muscle Antibodies  >28 publications with EIF3E and Muscle
 Lung Antibodies  >28 publications with EIF3E and Lung
 Brain Antibodies  >16 publications with EIF3E and Brain
 Vascular Antibodies  >13 publications with EIF3E and Vascular
 Kidney Antibodies  >13 publications with EIF3E and Kidney
 Prostate Antibodies  >12 publications with EIF3E and Prostate
 Embryonic Tissue Antibodies  >11 publications with EIF3E and Embryonic Tissue
 Lymph Antibodies  >9 publications with EIF3E and Lymph
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