• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

EIF3E Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8245451

MBS8245451 is an antibody targeting EIF3E. Reactive in Human, Mouse, Rat. Host: Rabbit. Rabbit polyclonal antibody to eIF3E More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
eIF3E, Polyclonal Antibody
Full Product Name
Anti-eIF3E Antibody
Product Gene Name
Product Synonym Names
EIF3S6; INT6; Eukaryotic translation initiation factor 3 subunit E; eIF3e; Eukaryotic translation initiation factor 3 subunit 6; Viral integration site protein INT-6 homolog; eIF-3 p48
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of eIF3E protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Molecular Weight
52,221 Da
OMIM
602210
3D Structure
ModBase 3D Structure for P60228
Immunogen
Recombinant full length protein of human eIF3E
Applications Tested/Suitable for anti-EIF3E antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-EIF3E antibody
WB: 1/500 - 1/2000; IF/ICC: 1/50 - 1/100

Western Blot (WB) of anti-EIF3E antibody

Western blot analysis of eIF3E expression in MCF7 (A), K562 (B), HepG2 (C) whole cell lysates.
Rabbit Anti-EIF3E Polyclonal Antibody – Cat# MBS8245451

Immunofluorescence (IF) of anti-EIF3E antibody

Immunofluorescent analysis of eIF3E staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-EIF3E Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS8245451

Other Notes

Small volumes of anti-EIF3E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF3E. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
eukaryotic translation initiation factor 3 subunit E
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 3 subunit E
NCBI Official Symbol
EIF3E
NCBI Official Synonym Symbols
INT6; EIF3S6; EIF3-P48; eIF3-p46
UniProt Protein Name
Eukaryotic translation initiation factor 3 subunit E
UniProt Synonym Protein Names
Eukaryotic translation initiation factor 3 subunit 6
UniProt Gene Name
EIF3E
UniProt Synonym Gene Names
eIF3e
UniProt Entry Name
EIF3E_HUMAN
NCBI Protein Information
eukaryotic translation initiation factor 3 subunit E

UniProt Comments for EIF3E

EIF3E: eukaryotic translation initiation factor 3 subunit 2. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. eIF-3 is composed of at least 12 different subunits. Phosphorylated by TGF-beta type II receptor.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 8q22-q23

Cellular Component: chromatin; cytoplasm; cytosol; eukaryotic translation initiation factor 3 complex; membrane; nucleoplasm; nucleus; PML body

Molecular Function: protein binding; protein N-terminus binding; translation initiation factor activity

Biological Process: formation of translation preinitiation complex; mRNA catabolic process, nonsense-mediated decay; negative regulation of translational initiation; regulation of translational initiation; translational initiation
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000