• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ENO1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9604393

Function: Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to… More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ENO1, Polyclonal Antibody
Full Product Name
ENO1 Antibody
Product Gene Name
anti-ENO1 antibody
Product Synonym Names
2 phospho D glycerate hydro lyase; 2-phospho-D-glycerate hydro-lyase; Alpha enolase; Alpha enolase like 1; Alpha-enolase; C myc promoter binding protein; C-myc promoter-binding protein; EC 4.2.1.11; eno1; ENO1L1; ENOA_HUMAN; Enolase 1 (alpha); Enolase 1 (alpha) like 1; Enolase 1; Enolase alpha; MBP 1; MBP-1; MBP1; MBPB1; MPB 1; MPB-1; MPB1; MYC promoter binding protein 1; NNE; Non neural enolase; Non-neural enolase; Phosphopyruvate hydratase; Plasminogen binding protein; Plasminogen-binding protein; PPH; Tau crystallin
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
ENO1 antibody detects endogenous levels of total ENO1
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
ENO1 peptide (MBS9623110) is used for blocking the activity of ENO1 antibody (MBS9604393)
Molecular Weight
Observed: 47 kDa
Predicted: 48 kDa
3D Structure
ModBase 3D Structure for P06733
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human ENO1
Subcellular Location
Nucleus and Cytoplasm. Cell Membrane. Cytoplasm > Myofibril > Sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
Tissue Specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ENO1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-ENO1 antibody
WB: 1:1000-3000
ICC/IF: 1:100-500
Peptide ELISA: 1:20000-1:40000

Western Blot (WB) of anti-ENO1 antibody

Western blot analysis of ENO1 using HeLa whole cell lysates
Rabbit Anti-ENO1 Polyclonal Antibody – Cat# MBS9604393

Immunofluorescene (IF) of anti-ENO1 antibody

MBS9604393 staining HeLa cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
Rabbit Anti-ENO1 Polyclonal Antibody, Immunofluorescene (IF) – Cat# MBS9604393

Other Notes

Small volumes of anti-ENO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ENO1 purchase

MBS9604393 is an antibody targeting ENO1. Also known as ENO1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ENO1. It may not necessarily be applicable to this product.

NCBI GI #
319996655
NCBI GeneID
2023
NCBI Accession #
NP_001188412.1
UniProt Primary Accession #
P06733
UniProt Secondary Accession #
P22712; Q16704; Q4TUS4; Q53FT9; Q53HR3; Q658M5; Q6GMP2; Q71V37; Q7Z3V6; Q8WU71; B2RD59
UniProt Related Accession #
P06733
GenBank Nucleotide #
NM_001201483.3
NCBI Official Full Name
c-myc promoter-binding protein-1 isoform MBP-1
NCBI Official Synonym Full Names
enolase 1
NCBI Official Symbol
ENO1
NCBI Official Synonym Symbols
NNE; PPH; MPB1; ENO1L1; HEL-S-17
UniProt Protein Name
Alpha-enolase
UniProt Synonym Protein Names
2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein
Protein Family
Alpha-enolase
UniProt Gene Name
ENO1
UniProt Synonym Gene Names
ENO1L1; MBPB1; MPB1; NNE
NCBI Protein Information
alpha-enolase; c-myc promoter-binding protein-1

NCBI Summary for ENO1

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
Show more

UniProt Comments for ENO1

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
Show more

Research Articles on ENO1

1. We propose that ENO1 is a useful indicator of parakeratosis and might have a potential role in cellular TJ barrier function in the epidermis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ENO1 antibody

 Products by Pathway  Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycolysis Pathway antibodies  Glycolysis Pathway Diagram
 Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies  Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram

Diseases associated with anti-ENO1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >71 publications with ENO1 and Neoplasms
 Carcinoma Antibodies  >33 publications with ENO1 and Carcinoma
 Nervous System Diseases Antibodies  >18 publications with ENO1 and Nervous System Diseases
 Brain Diseases Antibodies  >16 publications with ENO1 and Brain Diseases
 Adenocarcinoma Antibodies  >13 publications with ENO1 and Adenocarcinoma
 Breast Neoplasms Antibodies  >12 publications with ENO1 and Breast Neoplasms
 Arthritis, Rheumatoid Antibodies  >12 publications with ENO1 and Arthritis, Rheumatoid
 Carcinoma, Non-Small-Cell Lung Antibodies  >11 publications with ENO1 and Carcinoma, Non-Small-Cell Lung
 Inflammation Antibodies  >11 publications with ENO1 and Inflammation
 Neoplasms, Experimental Antibodies  >9 publications with ENO1 and Neoplasms, Experimental

Organs/Tissues associated with anti-ENO1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >43 publications with ENO1 and Blood
 Brain Antibodies  >28 publications with ENO1 and Brain
 Lung Antibodies  >26 publications with ENO1 and Lung
 Liver Antibodies  >15 publications with ENO1 and Liver
 Muscle Antibodies  >13 publications with ENO1 and Muscle
 Eye Antibodies  >12 publications with ENO1 and Eye
 Vascular Antibodies  >11 publications with ENO1 and Vascular
 Nerve Antibodies  >9 publications with ENO1 and Nerve
 Heart Antibodies  >7 publications with ENO1 and Heart
 Kidney Antibodies  >5 publications with ENO1 and Kidney
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000