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EPS8L1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8248136

MBS8248136 is an antibody targeting EPS8L1. Reactive in Human, Mouse, Rat. Host: Rabbit. Rabbit polyclonal antibody to EPS8L1 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EPS8L1, Polyclonal Antibody
Full Product Name
Anti-EPS8L1 Antibody
Product Gene Name
Product Synonym Names
DRC3; EPS8R1; Epidermal growth factor receptor kinase substrate 8-like protein 1; EPS8-like protein 1; Epidermal growth factor receptor pathway substrate 8-related protein 1; EPS8-related protein 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by affinity chromatography.
Specificity
Recognizes endogenous levels of EPS8L1 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
EPS8L1 peptide (MBS8247848) is used for blocking the activity of EPS8L1 antibody (MBS8248136)
OMIM
614987
3D Structure
ModBase 3D Structure for Q8TE68
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EPS8L1. The exact sequence is proprietary.
Applications Tested/Suitable for anti-EPS8L1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-EPS8L1 antibody
WB: 1/500 - 1/2000; Immunohistochemistry: 1/50 - 1/200; Immunofluorescence/Immunocytochemistry: 1/50 - 1/100

Western Blot (WB) of anti-EPS8L1 antibody

Western blot analysis of EPS8L1 expression in Hela (A), COLO205 (B), mouse uterus (C), rat placenta (D) whole cell lysates.
Rabbit Anti-EPS8L1 Polyclonal Antibody – Cat# MBS8248136

Immunohistochemistry (IHC) of anti-EPS8L1 antibody

Immunohistochemical analysis of EPS8L1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-EPS8L1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS8248136

Immunofluorescence (IF) of anti-EPS8L1 antibody

Immunofluorescent analysis of EPS8L1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-EPS8L1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS8248136

Other Notes

Small volumes of anti-EPS8L1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EPS8L1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
epidermal growth factor receptor kinase substrate 8-like protein 1 isoform b
NCBI Official Synonym Full Names
EPS8 like 1
NCBI Official Symbol
EPS8L1
NCBI Official Synonym Symbols
DRC3; EPS8R1; PP10566
UniProt Protein Name
Epidermal growth factor receptor kinase substrate 8-like protein 1
UniProt Synonym Protein Names
Epidermal growth factor receptor pathway substrate 8-related protein 1; EPS8-related protein 1
UniProt Gene Name
EPS8L1
UniProt Synonym Gene Names
DRC3; EPS8R1; EPS8-like protein 1; EPS8-related protein 1
UniProt Entry Name
ES8L1_HUMAN
NCBI Protein Information
epidermal growth factor receptor kinase substrate 8-like protein 1

NCBI Summary for EPS8L1

This gene encodes a protein that is related to epidermal growth factor receptor pathway substrate 8 (EPS8), a substrate for the epidermal growth factor receptor. The function of this protein is unknown. At least two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for EPS8L1

EPS8L1: Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton. Belongs to the EPS8 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: cell-cell adherens junction; cytoplasm; protein complex

Molecular Function: actin binding; protein binding; Rac guanyl-nucleotide exchange factor activity; Rho guanyl-nucleotide exchange factor activity; T cell receptor binding

Biological Process: regulation of Rho protein signal transduction; Rho protein signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EPS8L1 antibody

Diseases associated with anti-EPS8L1 antibody

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