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EZH1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758306

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
EZH1, Polyclonal Antibody
Full Product Name
Anti-EZH1 Antibody Picoband
Product Gene Name
anti-EZH1 antibody
Product Synonym Names
Heat shock protein beta-8; Heat shock protein beta-8; HspB8; Alpha-crystallin C chain; E2-induced gene 1 protein; Protein kinase H11; Small stress protein-like protein HSP22; HSPB8; CRYAC, E2IG1, HSP22; PP1629
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Predominantly expressed in skeletal muscle and heart.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
85,271 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (40852293..40897071, complement). Location: 17q21.1-q21.3
OMIM
601674
3D Structure
ModBase 3D Structure for Q92800
Immunogen
E coli-derived human EZH1 recombinant protein (Position: R27-E485).
Subcellular Localization
Cytoplasm, Nucleus, Translocates to nuclear foci during heat shock
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins
Protein Function
Displays temperature-dependent chaperone activity.

Product Categories/Family for anti-EZH1 antibody

Primary Antibodies; Chaperones; Heat Shock Proteins; Neurodegenerative Disease; Neurology Process; Neuroscience; Protein Trafficking; Signal Transduction
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-EZH1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-EZH1 antibody
WB: 0.25-0.5ug/ml, Human, Mouse, Rat
ICC/IF: 5ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-EZH1 antibody

Figure 1. Western blot analysis of EZH1 using anti-EZH1 antibody (MBS1758306).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human HL-60 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human Daudi whole cell lysates,
Lane 5: human Hacat whole cell lysates,
Lane 6: human Hela whole cell lysates,
Lane 7: human MOLT-4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EZH1 antigen affinity purified polyclonal antibody (#MBS1758306) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for EZH1 at approximately 100 kDa, 60 kDa. The expected band size for EZH1 is at 68 kDa.
Rabbit Anti-EZH1 Polyclonal Antibody – Cat# MBS1758306

Western Blot (WB) of anti-EZH1 antibody

Figure 2. Western blot analysis of EZH1 using anti-EZH1 antibody (MBS1758306).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: rat C6 whole cell lysates,
Lane 3: mouse testis tissue lysates,
Lane 4: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EZH1 antigen affinity purified polyclonal antibody (#MBS1758306) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for EZH1 at approximately 110 kDa, 60 kDa. The expected band size for EZH1 is at 68 kDa.
Rabbit Anti-EZH1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS1758306

Immunofluorescence (IF) of anti-EZH1 antibody

Figure 3. IF analysis of EZH1 using anti-EZH1 antibody (MBS1758306) and anti-Tubulin Alpha antibody (M03989-3).
EZH1 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (#MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-EZH1 Antibody (MBS1758306) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Rabbit Anti-EZH1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1758306

Flow Cytometry (FC/FACS) of anti-EZH1 antibody

Figure 4. Flow Cytometry analysis of RT4 cells using anti-EZH1 antibody (MBS1758306).
Overlay histogram showing RT4 cells stained with MBS1758306 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EZH1 Antibody (MBS1758306, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Rabbit Anti-EZH1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758306

Product References and Citations for anti-EZH1 antibody

1. Abel, K. J., Brody, L. C., Valdes, J. M., Erdos, M. R., McKinley, D. R., Castilla, L. H., Merajver, S. D., Couch, F. J., Friedman, L. S., Ostermeyer, E. A., Lynch, E. D., King, M.-C., Welcsh, P. L., Osborne-Lawrence, S., Spillman, M., Bowcock, A. M., Collins, F. S., Weber, B. L. Characterization of EZH1, a human homolog of Drosophila enhancer of zeste near BRCA1. Genomics 37: 161-171, 1996. 2. Laible, G., Haynes, A. R., Lebersorger, A., O'Carroll, D., Mattei, M.-G., Denny, P., Brown, S. D. M., Jenuwein, T. The murine polycomb-group genes Ezh1 and Ezh2 map close to Hox gene clusters on mouse chromosomes 11 and 6. Mammalian Genome 10: 311-314, 1999.3. Maier, V. K., Feeney, C. M., Taylor, J. E., Creech, A. L., Qiao, J. W., Szanto, A., Das, P. P., Chevrier, N., Cifuentes-Rojas, C., Orkin, S. H., Carr, S. A., Jaffe, J. D., Mertins, P., Lee, J. T. Functional proteomic analysis of repressive histone methyltransferase complexes reveals ZNF518B as a G9A regulator. Molec. Cell. Proteomics 14: 1435-1446, 2015.

Other Notes

Small volumes of anti-EZH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EZH1 purchase

MBS1758306 is an antibody targeting EZH1. Also known as Anti-EZH1 Antibody Picoband. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EZH1. It may not necessarily be applicable to this product.

NCBI GI #
19923202
NCBI GeneID
2145
NCBI Accession #
NP_001982.2
UniProt Primary Accession #
Q92800
UniProt Secondary Accession #
O43287; Q14459; Q53XP3; A6NCH6; B4DIJ1; B4DIZ7; B4DSS2; B4E3R7
UniProt Related Accession #
Q92800
GenBank Nucleotide #
NM_001991.3
NCBI Official Full Name
histone-lysine N-methyltransferase EZH1
NCBI Official Synonym Full Names
enhancer of zeste homolog 1 (Drosophila)
NCBI Official Symbol
EZH1
NCBI Official Synonym Symbols
KMT6B
UniProt Protein Name
Histone-lysine N-methyltransferase EZH1
UniProt Synonym Protein Names
ENX-2; Enhancer of zeste homolog 1
Protein Family
Histone-lysine N-methyltransferase
UniProt Gene Name
EZH1
UniProt Synonym Gene Names
KIAA0388
UniProt Entry Name
EZH1_HUMAN
NCBI Protein Information
histone-lysine N-methyltransferase EZH1; ENX-2

NCBI Summary for EZH1

EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).[supplied by OMIM, Mar 2009]

UniProt Comments for EZH1

EZH1: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. Belongs to the histone-lysine methyltransferase family. EZ subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 17q21.1-q21.3

Cellular Component: nucleoplasm; ESC/E(Z) complex

Molecular Function: chromatin binding; histone lysine N-methyltransferase activity (H3-K27 specific)

Biological Process: anatomical structure morphogenesis; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter
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Research Articles on EZH1

1. Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EZH1 antibody

 Reference Product  PubMed Publications
 EED antibody  >7 publications with EZH1 and EED

Diseases associated with anti-EZH1 antibody

 Disease Name  Pubmed Publications
 Neoplasms, Experimental Antibodies  >2 publications with EZH1 and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >2 publications with EZH1 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >2 publications with EZH1 and Cell Transformation, Neoplastic
 Ovarian Diseases Antibodies  >1 publications with EZH1 and Ovarian Diseases
 Precancerous Conditions Antibodies  >1 publications with EZH1 and Precancerous Conditions

Organs/Tissues associated with anti-EZH1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5 publications with EZH1 and Blood
 Muscle Antibodies  >4 publications with EZH1 and Muscle
 Kidney Antibodies  >3 publications with EZH1 and Kidney
 Skin Antibodies  >3 publications with EZH1 and Skin
 Nerve Antibodies  >3 publications with EZH1 and Nerve
 Embryonic Tissue Antibodies  >3 publications with EZH1 and Embryonic Tissue
 Heart Antibodies  >2 publications with EZH1 and Heart
 Bone Antibodies  >2 publications with EZH1 and Bone
 Liver Antibodies  >2 publications with EZH1 and Liver
 Bone Marrow Antibodies  >2 publications with EZH1 and Bone Marrow
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