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GEMIN5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9142621

Background: This gene encodes a WD repeat protein that is a component of the survival of motor neurons (SMN) complex. The SMN complex plays a critical role in mRNA splicing through the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), and… More details

$225 / 0.02 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GEMIN5, Polyclonal Antibody
Full Product Name
GEMIN5 Rabbit pAb
Product Gene Name
Product Synonym Names
GEMIN5; GEMIN-5
Immunogen Sequence
RTPVKLESID GNEEESMKEN SGPVENGVSD QEGEEQAREP ELPCGLAPAV SREPVICTPV SSGFEKSKVT INNKVILLKK EPPKEKPETL IKKRKARSLL PLSTSLDHRS KEELHQDCLV LATAKHSREL NEDVSADVEE RFHLGLFTDR ATLYRMIDIE GKGHLENGHP ELFHQLMLWK GDLKGVLQTA AERGELTDNL V
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purity/Purification
Affinity purification
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Molecular Weight
168,589 Da
OMIM
607005
3D Structure
ModBase 3D Structure for Q8TEQ6
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 750-950 of human GEMIN5 (NP_056280.2).
Positive Samples
HeLa
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-GEMIN5 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-GEMIN5 antibody
WB: 1:500-1:2000
IHC: 1:50-1:100

Western Blot (WB) of anti-GEMIN5 antibody

Western blot analysis of extracts of HeLa cells, using GEMIN5 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Rabbit Anti-GEMIN5 Polyclonal Antibody – Cat# MBS9142621

Immunohistochemistry (IHC) of anti-GEMIN5 antibody

Immunohistochemistry of paraffin-embedded rat kidney using GEMIN5 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-GEMIN5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9142621

Immunohistochemistry (IHC) of anti-GEMIN5 antibody

Immunohistochemistry of paraffin-embedded rat heart using GEMIN5 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-GEMIN5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9142621

Immunohistochemistry (IHC) of anti-GEMIN5 antibody

Immunohistochemistry of paraffin-embedded human placenta using GEMIN5 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-GEMIN5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9142621

Other Notes

Small volumes of anti-GEMIN5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GEMIN5 purchase

MBS9142621 is an antibody targeting GEMIN5. Also known as GEMIN5 Rabbit pAb. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GEMIN5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
gem-associated protein 5 isoform 2
NCBI Official Synonym Full Names
gem nuclear organelle associated protein 5
NCBI Official Symbol
GEMIN5
NCBI Official Synonym Symbols
GEMIN-5
UniProt Protein Name
Gem-associated protein 5
UniProt Gene Name
GEMIN5
UniProt Synonym Gene Names
Gemin5
UniProt Entry Name
GEMI5_HUMAN
NCBI Protein Information
gem-associated protein 5

NCBI Summary for GEMIN5

This gene encodes a WD repeat protein that is a component of the survival of motor neurons (SMN) complex. The SMN complex plays a critical role in mRNA splicing through the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), and may also mediate the assembly and transport of other classes of ribonucleoproteins. The encoded protein is the snRNA-binding component of the SMN complex. Dysregulation of this gene may play a role in alternative mRNA splicing and tumor cell motility. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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