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GIT1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9608977

Function: GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell… More details

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Product Overview

Online Datasheet in PDF
Product Name
GIT1, Polyclonal Antibody
Full Product Name
GIT1 Antibody
Product Gene Name
anti-GIT1 antibody
Product Synonym Names
ARF GAP GIT1; ARF GTPase activating protein GIT1; ARF GTPase-activating protein GIT1; CAT 1; CAT-1; CaT1; Cool associated and tyrosine phosphorylated protein 1; Cool-associated and tyrosine-phosphorylated protein 1; G protein coupled receptor kinase interacting ArfGAP 1; G protein coupled receptor kinase interactor 1; G protein-coupled receptor kinase-interactor 1; GIT1; GIT1_HUMAN; GRK interacting protein 1; GRK-interacting protein 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
GIT1 antibody detects endogenous levels of total GIT1
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
GIT1 peptide (MBS9627689) is used for blocking the activity of GIT1 antibody (MBS9608977)
Molecular Weight
Observed: 84 kDa
Predicted: 85 kDa
3D Structure
ModBase 3D Structure for Q9Y2X7
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human GIT1
Subcellular Location
Cytoplasm. Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (88%), Rabbit (100%), Dog (100%), Chicken (82%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-GIT1 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-GIT1 antibody
WB: 1:1000-3000
IHC: 1:200
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-GIT1 antibody

MBS9608977 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Rabbit Anti-GIT1 Polyclonal Antibody – Cat# MBS9608977

Immunohistochemistry (IHC) of anti-GIT1 antibody

MBS9608977 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-GIT1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9608977

Western Blot (WB) of anti-GIT1 antibody

Western blot analysis GIT1 using HuvEc whole cell lysates
Rabbit Anti-GIT1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9608977

Other Notes

Small volumes of anti-GIT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GIT1 purchase

MBS9608977 is an antibody targeting GIT1. Also known as GIT1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GIT1. It may not necessarily be applicable to this product.

NCBI GI #
146231998
NCBI GeneID
28964
NCBI Accession #
NP_001078923.1
UniProt Primary Accession #
Q9Y2X7
UniProt Secondary Accession #
Q86SS0; Q9BRJ4; B4DGU9; B4DSV3
UniProt Related Accession #
Q9Y2X7
GenBank Nucleotide #
NM_001085454.1
NCBI Official Full Name
ARF GTPase-activating protein GIT1 isoform 1
NCBI Official Synonym Full Names
GIT ArfGAP 1
NCBI Official Symbol
GIT1
UniProt Protein Name
ARF GTPase-activating protein GIT1
UniProt Synonym Protein Names
Cool-associated and tyrosine-phosphorylated protein 1; CAT-1; CAT1; G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1
Protein Family
Adenylate cyclase activation protein
UniProt Gene Name
GIT1
UniProt Synonym Gene Names
ARF GAP GIT1; CAT-1; CAT1
NCBI Protein Information
ARF GTPase-activating protein GIT1

UniProt Comments for GIT1

GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve ENTR1 and PTPN13 ().
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Research Articles on GIT1

1. There is significant interaction effects of the GIT1 and DRD4 gene variants on impulsivity in Attention Deficit Hyperactivity Disorder.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GIT1 antibody

 Reference Product  PubMed Publications
 ARF6 antibody  >19 publications with GIT1 and ARF6
 PXN antibody  >17 publications with GIT1 and PXN
 ARHGEF7 antibody  >11 publications with GIT1 and ARHGEF7
 ADRBK1 antibody  >9 publications with GIT1 and ADRBK1
 PTK2 antibody  >7 publications with GIT1 and PTK2
 PTPRZ1 antibody  >4 publications with GIT1 and PTPRZ1
 EPHA2 antibody  >2 publications with GIT1 and EPHA2
 NCK1 antibody  >2 publications with GIT1 and NCK1
 ARHGEF6 antibody  >1 publications with GIT1 and ARHGEF6

Pathways associated with anti-GIT1 antibody

 Products by Pathway  Pathway Diagram
 Arf6 Signaling Events Pathway antibodies  Arf6 Signaling Events Pathway Diagram
 Aurora A Signaling Pathway antibodies  Aurora A Signaling Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 EPH-Ephrin Signaling Pathway antibodies  EPH-Ephrin Signaling Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Ephrin Signaling Pathway antibodies  Ephrin Signaling Pathway Diagram
 Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway antibodies  Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway Diagram

Diseases associated with anti-GIT1 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >8 publications with GIT1 and Carcinoma
 Attention Deficit Disorder with Hyperactivity Antibodies  >4 publications with GIT1 and Attention Deficit Disorder with Hyperactivity
 Breast Neoplasms Antibodies  >3 publications with GIT1 and Breast Neoplasms
 Inflammation Antibodies  >3 publications with GIT1 and Inflammation
 Lung Neoplasms Antibodies  >3 publications with GIT1 and Lung Neoplasms
 Learning Disorders Antibodies  >2 publications with GIT1 and Learning Disorders
 Hemorrhage Antibodies  >2 publications with GIT1 and Hemorrhage
 Carcinoma, Hepatocellular Antibodies  >2 publications with GIT1 and Carcinoma, Hepatocellular
 Carcinoma, Squamous Cell Antibodies  >2 publications with GIT1 and Carcinoma, Squamous Cell
 Neurologic Manifestations Antibodies  >2 publications with GIT1 and Neurologic Manifestations

Organs/Tissues associated with anti-GIT1 antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >28 publications with GIT1 and Vascular
 Brain Antibodies  >24 publications with GIT1 and Brain
 Blood Antibodies  >18 publications with GIT1 and Blood
 Muscle Antibodies  >15 publications with GIT1 and Muscle
 Nerve Antibodies  >11 publications with GIT1 and Nerve
 Lung Antibodies  >9 publications with GIT1 and Lung
 Bone Antibodies  >9 publications with GIT1 and Bone
 Embryonic Tissue Antibodies  >6 publications with GIT1 and Embryonic Tissue
 Kidney Antibodies  >6 publications with GIT1 and Kidney
 Stomach Antibodies  >4 publications with GIT1 and Stomach
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