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GPR109 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9606342

Function: Receptor for 3-OH-octanoid acid mediates a negative feedback regulation of adipocyte lipolysis to counteract prolipolytic influences under conditions of physiological or pathological increases in beta-oxidation rates. Acts as a low affinity receptor… More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GPR109, Polyclonal Antibody
Full Product Name
GPR109 Antibody
Product Gene Name
anti-GPR109 antibody
Product Synonym Gene Name
HCAR3
Product Synonym Names
G protein coupled receptor 109B; G protein coupled receptor HM74; G protein coupled receptor HM74B; G-protein coupled receptor 109B; G-protein coupled receptor HM74; G-protein coupled receptor HM74B; GPR109B; HCA3; HCAR3; HCAR3_HUMAN; HM74; HM74B; Hydroxycarboxylic acid receptor 3; Niacin receptor 2; NIACR2; Nicotinic acid receptor 2; Puma g; PUMAG; G protein coupled receptor 109A; G protein coupled receptor HM74a; G protein coupled receptor Hm74b; G-protein coupled receptor 109A; G-protein coupled receptor HM74A; G109A_HUMAN; GPR 109A; Gpr109a; GPR109A protein; HCA2; HCAR2; HM 74; HM 74a; HM 74b; Hm74; HM74a; HM74b; hydroxycarboxylic acid receptor 2; Interferon gamma inducible gene Puma g; Niacin receptor 1; NIACR1; Nicotinic acid receptor 1; Nicotinic acid receptor; Protein upregulated in macrophages by IFNG, mouse, homolog of; Puma g; Pumag; rHM74b
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
GPR109 antibody detects endogenous levels of total GPR109
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
GPR109 peptide (MBS9625059) is used for blocking the activity of GPR109 antibody (MBS9606342)
Molecular Weight
Observed: 45 kD
Predicted: 45 kDa
3D Structure
ModBase 3D Structure for P49019
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide
Subcellular Location
Cell Membrane
Tissue Specificity
Expression largely restricted to adipose tissue and spleen.
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-GPR109 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-GPR109 antibody
WB: 1:500-1:1000
IF/ICC: 1:100-1:500

Western Blot (WB) of anti-GPR109 antibody

Western blot analysis of extracts from RAW264.7 cells, using GPR109 antibody.
Rabbit Anti-GPR109 Polyclonal Antibody – Cat# MBS9606342

Immunofluorescene (IF) of anti-GPR109 antibody

MBS9606342 staining RAW264.7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Rabbit Anti-GPR109 Polyclonal Antibody, Immunofluorescene (IF) – Cat# MBS9606342

Western Blot (WB) of anti-GPR109 antibody

Western blot analysis of extracts from rat spleen, using GPR109 Antibody.
Rabbit Anti-GPR109 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9606342

Other Notes

Small volumes of anti-GPR109 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GPR109 purchase

MBS9606342 is an antibody targeting GPR109. Also known as GPR109 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GPR109. It may not necessarily be applicable to this product.

NCBI GI #
157738694
NCBI GeneID
8843
NCBI Accession #
NP_006009.2
UniProt Primary Accession #
P49019
UniProt Secondary Accession #
Q8NGE4; A8K4G5; B2R830; E9PI97
UniProt Related Accession #
P49019
GenBank Nucleotide #
NM_006018.2
NCBI Official Full Name
hydroxycarboxylic acid receptor 3
NCBI Official Synonym Full Names
hydroxycarboxylic acid receptor 3
NCBI Official Symbol
HCAR3
NCBI Official Synonym Symbols
HCA3; HM74; PUMAG; Puma-g; GPR109B
UniProt Protein Name
Hydroxycarboxylic acid receptor 3
UniProt Synonym Protein Names
G-protein coupled receptor 109B; G-protein coupled receptor HM74; G-protein coupled receptor HM74B; Niacin receptor 2; Nicotinic acid receptor 2
UniProt Gene Name
HCAR3
UniProt Synonym Gene Names
GPR109B; HCA3; HM74B; NIACR2
NCBI Protein Information
hydroxycarboxylic acid receptor 3

UniProt Comments for GPR109

Receptor for 3-OH-octanoid acid mediates a negative feedback regulation of adipocyte lipolysis to counteract prolipolytic influences under conditions of physiological or pathological increases in beta-oxidation rates. Acts as a low affinity receptor for nicotinic acid. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet.
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Research Articles on GPR109

1. new insights into the G protein coupling profiles of the HCA receptors and the function of the receptor's C terminus

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-GPR109 antibody

 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Hydroxycarboxylic Acid-binding Receptors Pathway antibodies  Hydroxycarboxylic Acid-binding Receptors Pathway Diagram
 Metabolic Disorders Of Biological Oxidation Enzymes Pathway antibodies  Metabolic Disorders Of Biological Oxidation Enzymes Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram

Diseases associated with anti-GPR109 antibody

 Disease Name  Pubmed Publications
 Atherosclerosis Antibodies  >7 publications with GPR109 and Atherosclerosis
 Dyslipidemias Antibodies  >6 publications with GPR109 and Dyslipidemias
 Carcinoma, Squamous Cell Antibodies  >3 publications with GPR109 and Carcinoma, Squamous Cell
 Disease Models, Animal Antibodies  >2 publications with GPR109 and Disease Models, Animal
 Fatty Liver Antibodies  >1 publications with GPR109 and Fatty Liver
 Skin Neoplasms Antibodies  >1 publications with GPR109 and Skin Neoplasms

Organs/Tissues associated with anti-GPR109 antibody

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >8 publications with GPR109 and Skin
 Blood Antibodies  >7 publications with GPR109 and Blood
 Lung Antibodies  >2 publications with GPR109 and Lung
 Brain Antibodies  >1 publications with GPR109 and Brain
 Spleen Antibodies  >1 publications with GPR109 and Spleen
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