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HSPA5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS806289

Background Info: Detects a 78kDa protein corresponding to the molecular mass of Grp78 in SDS PAGE immunoblots.Scientific Background: GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain… More details

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Product Overview

Product Name
GRP78 (Hspa5), Polyclonal Antibody
Full Product Name
GRP78 Antibody: ATTO 633
Product Gene Name
anti-Hspa5 antibody
Product Synonym Names
BIP; Grp78; HspA5; MIF2; immunoglobulin heavy chain binding protein
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human (weak), Mouse, Rat
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Preparation and Storage
-20 degree C
Molecular Weight
72,347 Da
3D Structure
ModBase 3D Structure for P06761
Conjugate
ATTO 633
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide

Product Categories/Family for anti-Hspa5 antibody

Chaperones; Heat Shock; Organelle Markers; Trafficking
Applications Tested/Suitable for anti-Hspa5 antibody
Western Blot (WB)
Application Notes for anti-Hspa5 antibody
1:1000 (WB)

Immunocytochemistry (ICC) of anti-Hspa5 antibody

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 Polyclonal Antibody . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody at 1:60 for 12 hours at 4 degree C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.
Rabbit Anti-HSPA5 Polyclonal Antibody – Cat# MBS806289

Immunocytochemistry (ICC) of anti-Hspa5 antibody

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 Polyclonal Antibody . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody at 1:60 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.
Rabbit Anti-HSPA5 Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS806289

Western Blot (WB) of anti-Hspa5 antibody

Western blot analysis of Rat Brain cell lysates showing detection of ~78 kDa GRP78 protein using Rabbit Anti-GRP78 Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.
Rabbit Anti-HSPA5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS806289

Product References and Citations for anti-Hspa5 antibody

1. Cho S., et al. (2007). Mol Cell Biol 27(1): 368-83. 2. Yang Y., et al. (1998) J Biol Chem 273: 25552-25555. 3. Luo S., et al (2006) 26 (15): 5688-97. 4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi R., et al. (1999) Anticancer Res. 19:4333- 4336. 6. Laquerre S., et al. (1998) J. Virology 72: 4940-4949. 7. Dong D., et al. (2005) Cancer Res 65(13): 5785-91.

Other Notes

Small volumes of anti-Hspa5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Hspa5 purchase

MBS806289 is an antibody targeting Hspa5. Also known as GRP78 Antibody: ATTO 633. Reactive in Human (weak), Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Hspa5. It may not necessarily be applicable to this product.

NCBI GI #
25742763
NCBI GeneID
25617
NCBI Accession #
NP_037215.1
UniProt Primary Accession #
P06761
UniProt Related Accession #
P06761
GenBank Nucleotide #
NM_013083.2
NCBI Official Full Name
78 kDa glucose-regulated protein
NCBI Official Synonym Full Names
heat shock protein 5
NCBI Official Symbol
Hspa5
NCBI Official Synonym Symbols
BIP; GRP78
UniProt Protein Name
78 kDa glucose-regulated protein
UniProt Synonym Protein Names
Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP; Steroidogenesis-activator polypeptide
Protein Family
Transmembrane protein
UniProt Gene Name
Hspa5
UniProt Synonym Gene Names
Grp78; GRP-78; BiP
UniProt Entry Name
GRP78_RAT
NCBI Protein Information
78 kDa glucose-regulated protein; GRP 78; GRP-78; heat shock 70kD protein 5; heat shock 70 kDa protein 5; steroidogenesis-activator polypeptide; immunoglobulin heavy chain-binding protein; heat shock 70kDa protein 5 (glucose-regulated protein)

NCBI Summary for Hspa5

secreted protein of the endoplasmic reticulum; may be involved in the assembly of secreted and membrane-bound proteins [RGD, Feb 2006]

UniProt Comments for Hspa5

GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.

Protein type: Chaperone; Heat shock protein

Cellular Component: signalosome; endoplasmic reticulum membrane; smooth endoplasmic reticulum; cell surface; focal adhesion; mitochondrion; endoplasmic reticulum; endoplasmic reticulum lumen; ER-Golgi intermediate compartment; membrane; plasma membrane; melanosome; integral to endoplasmic reticulum membrane; midbody; nucleus; myelin sheath

Molecular Function: protein domain specific binding; protein binding; enzyme binding; ubiquitin protein ligase binding; unfolded protein binding; ribosome binding; misfolded protein binding; glycoprotein binding; ATP binding

Biological Process: cellular response to glucose starvation; substantia nigra development; unfolded protein response, activation of signaling protein activity; cerebellum structural organization; proteolysis involved in cellular protein catabolic process; positive regulation of protein ubiquitination; cerebellar Purkinje cell layer development; negative regulation of transforming growth factor beta receptor signaling pathway; ER overload response; negative regulation of apoptosis; positive regulation of cell migration
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Research Articles on Hspa5

1. Over-expression of GRP78 in lungs may be the critical pathogenic factor for hepatopulmonary syndrome.GRP78 may play a crucial role in promoting the pulmonary microvascular remodeling in a rat model of hepatopulmonary syndrome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Hspa5 antibody

 Reference Product  PubMed Publications
 Atf6 antibody  >29 publications with Hspa5 and Atf6
 Atf4 antibody  >26 publications with Hspa5 and Atf4
 Hyou1 antibody  >7 publications with Hspa5 and Hyou1
 Sil1 antibody  >5 publications with Hspa5 and Sil1
 Tra1 antibody  >3 publications with Hspa5 and Tra1
 Eif2ak3 antibody  >2 publications with Hspa5 and Eif2ak3
 Nfya antibody  >1 publications with Hspa5 and Nfya

Pathways associated with anti-Hspa5 antibody

 Products by Pathway  Pathway Diagram
 ATF6-alpha Activates Chaperone Genes Pathway antibodies  ATF6-alpha Activates Chaperone Genes Pathway Diagram
 ATF6-alpha Activates Chaperones Pathway antibodies  ATF6-alpha Activates Chaperones Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Prion Diseases Pathway antibodies  Prion Diseases Pathway Diagram

Diseases associated with anti-Hspa5 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >59 publications with Hspa5 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >56 publications with Hspa5 and Disease Models, Animal
 Nervous System Diseases Antibodies  >55 publications with Hspa5 and Nervous System Diseases
 Inflammation Antibodies  >33 publications with Hspa5 and Inflammation
 Liver Diseases Antibodies  >21 publications with Hspa5 and Liver Diseases
 Heart Diseases Antibodies  >20 publications with Hspa5 and Heart Diseases
 Necrosis Antibodies  >19 publications with Hspa5 and Necrosis
 Kidney Diseases Antibodies  >13 publications with Hspa5 and Kidney Diseases
 Fibrosis Antibodies  >10 publications with Hspa5 and Fibrosis
 Fatty Liver Antibodies  >10 publications with Hspa5 and Fatty Liver

Organs/Tissues associated with anti-Hspa5 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >75 publications with Hspa5 and Brain
 Muscle Antibodies  >48 publications with Hspa5 and Muscle
 Heart Antibodies  >38 publications with Hspa5 and Heart
 Liver Antibodies  >38 publications with Hspa5 and Liver
 Kidney Antibodies  >30 publications with Hspa5 and Kidney
 Artery Antibodies  >17 publications with Hspa5 and Artery
 Pancreas Antibodies  >16 publications with Hspa5 and Pancreas
 Lung Antibodies  >14 publications with Hspa5 and Lung
 Testis Antibodies  >11 publications with Hspa5 and Testis
 Ovary Antibodies  >10 publications with Hspa5 and Ovary
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