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H1F0 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS129625
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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core… More details

$245 / 0.02 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
H1F0, Polyclonal Antibody
Full Product Name
H1F0 Polyclonal Antibody
Product Gene Name
Product Synonym Names
H10; H1FV
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Molecular Weight
194
OMIM
142708
3D Structure
ModBase 3D Structure for P07305
Species
Human
Immunogen
Recombinant protein of human H1F0
Calculated Molecular Weight
21kDa

Product Categories/Family for anti-H1F0 antibody

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-H1F0 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-H1F0 antibody
WB: 1:500 - 1:2000
IF: 1:50 - 1:200

Western Blot (WB) of anti-H1F0 antibody

Western blot analysis of extracts of various cell lines, using H1F0 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.
Rabbit Anti-H1F0 Polyclonal Antibody – Cat# MBS129625

Immunohistochemistry (IHC) of anti-H1F0 antibody

Immunohistochemistry of paraffin-embedded mouse brain using H1F0 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-H1F0 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS129625

Immunohistochemistry (IHC) of anti-H1F0 antibody

Immunohistochemistry of paraffin-embedded human stomach using H1F0 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-H1F0 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS129625

Immunohistochemistry (IHC) of anti-H1F0 antibody

Immunohistochemistry of paraffin-embedded rat liver using H1F0 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-H1F0 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS129625

Immunohistochemistry (IHC) of anti-H1F0 antibody

Immunohistochemistry of paraffin-embedded mouse kidney using H1F0 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-H1F0 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS129625

Other Notes

Small volumes of anti-H1F0 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for H1F0 purchase

MBS129625 is an antibody targeting H1F0. Also known as H1F0 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for H1F0. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Histone H1.0
NCBI Official Synonym Full Names
H1 histone family, member 0
NCBI Official Symbol
H1F0
NCBI Official Synonym Symbols
H10; H1FV
UniProt Protein Name
Histone H1.0
UniProt Synonym Protein Names
Histone H1'; Histone H1(0)Histone H1.0, N-terminally processed
Protein Family
UniProt Gene Name
H1F0
UniProt Synonym Gene Names
H1FV
UniProt Entry Name
H10_HUMAN
NCBI Protein Information
histone H1.0; histone H1'; histone H1(0); H1.0, H1(0), H1-0

UniProt Comments for H1F0

H1F0: Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. Belongs to the histone H1/H5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; Golgi apparatus; nuclear chromatin; nucleosome; nucleus; actin cytoskeleton

Molecular Function: protein binding; chromatin DNA binding

Biological Process: nucleosome assembly; apoptosis; DNA fragmentation during apoptosis; cell structure disassembly during apoptosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-H1F0 antibody

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