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HIST1H3A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS7106639

MBS7106639 is an antibody targeting HIST1H3A. Also known as HIST1H3A (Ab-27) Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$235 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HIST1H3A, Polyclonal Antibody
Full Product Name
HIST1H3A (Ab-27) Antibody
Product Gene Name
Product Synonym Names
Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST1H3A; H3FA; AND; HIST1H3B; H3FL; AND; HIST1H3C; H3FC; AND; HIST1H3D; H3FB; AND; HIST1H3E; H3FD; AND; HIST1H3F; H3FI; AND; HIST1H3G; H3FH; AND; HIST1H3H; H3FK; AND; HIST1H3I; H3FF; AND; HIST1H3J; H3FJ
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Antigen Affinity Purified
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
Molecular Weight
15,404 Da
OMIM
X00090 Genomic DNA
3D Structure
ModBase 3D Structure for P68431
Immunogen
Peptide sequence around site of Lys (27) derived from human Histone H3.1.
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-HIST1H3A antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)
Application Notes for anti-HIST1H3A antibody
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:200

Western Blot (WB) of anti-HIST1H3A antibody

Western Blot
Positive WB detected in: NIH/3T3 cell acid extracts, Hela cell acid extracts, A549 cell acid extracts, Jurkat cell acid extracts, PC3 cell acid extracts, HEK293 cell acid extracts
All lanes: HIST1H3A antibody at 2ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 16 KDa
Observed band size: 16 KDa
Rabbit Anti-HIST1H3A Polyclonal Antibody – Cat# MBS7106639

Immunohistochemistry (IHC) of anti-HIST1H3A antibody

Immunohistochemistry of paraffin-embedded human colon cancer using MBS7106639 at dilution of 1:100
Rabbit Anti-HIST1H3A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS7106639

Immunohistochemistry (IHC) of anti-HIST1H3A antibody

Immunohistochemistry of paraffin-embedded human lung cancer using MBS7106639 at dilution of 1:100
Rabbit Anti-HIST1H3A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS7106639

Immunofluorescence (IF) of anti-HIST1H3A antibody

Immunofluorescent analysis of Hela cells using MBS7106639 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Rabbit Anti-HIST1H3A Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS7106639

Chromatin Immunoprecipitation (ChIP) of anti-HIST1H3A antibody

Chromatin Immunoprecipitation Hela(4^106)were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8ug anti-HIST1H3A (MBS7106639)or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.
Rabbit Anti-HIST1H3A Polyclonal Antibody, Chromatin Immunoprecipitation (ChIP) – Cat# MBS7106639

Other Notes

Small volumes of anti-HIST1H3A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HIST1H3A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
histone H3.1
NCBI Official Synonym Full Names
histone cluster 1 H3 family member a
NCBI Official Symbol
HIST1H3A
NCBI Official Synonym Symbols
H3/A; H3FA
UniProt Protein Name
Histone H3.1
UniProt Synonym Protein Names
Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
UniProt Gene Name
HIST1H3A
UniProt Synonym Gene Names
H3FA
NCBI Protein Information
histone H3.1

NCBI Summary for HIST1H3A

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
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UniProt Comments for HIST1H3A

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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