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ICT1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9603433

Function: Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion,… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ICT1, Polyclonal Antibody
Full Product Name
ICT1 Antibody
Product Gene Name
anti-ICT1 antibody
Product Synonym Gene Name
MRPL58
Product Synonym Names
Digestion substraction 1; DS-1; DS1; Ict1; ICT1_HUMAN; Immature colon carcinoma transcript 1; Immature colon carcinoma transcript 1 protein; mitochondrial; Peptidyl-tRNA hydrolase ICT1; Peptidyl-tRNA hydrolase ICT1, mitochondrial
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
ICT1 antibody detects endogenous levels of ICT1.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
ICT1 peptide (MBS9620910) is used for blocking the activity of ICT1 antibody (MBS9603433)
Molecular Weight
Observed: 21-24 kDa
Predicted: 24 kDa
3D Structure
ModBase 3D Structure for Q14197
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human ICT1.
Subcellular Location
Mitochondrion.
Tissue Specificity
Down-regulated during the in vitro differentiation of HT29-D4 colon carcinoma cells.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (83%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (92%), Dog (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ICT1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ICT1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Immunohistochemistry (IHC) of anti-ICT1 antibody

MBS9603433 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-ICT1 Polyclonal Antibody – Cat# MBS9603433

Western Blot (WB) of anti-ICT1 antibody

Western blot analysis of extracts of SKOV-3 cells, using ICT1 antibody.
Rabbit Anti-ICT1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9603433

Other Notes

Small volumes of anti-ICT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ICT1 purchase

MBS9603433 is an antibody targeting ICT1. Also known as ICT1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ICT1. It may not necessarily be applicable to this product.

NCBI GI #
737676317
NCBI GeneID
3396
NCBI Accession #
NP_001290194.1
UniProt Primary Accession #
Q14197
UniProt Secondary Accession #
Q53HM7; Q53Y11; B2RAD1
UniProt Related Accession #
Q14197
GenBank Nucleotide #
NM_001303265.1
NCBI Official Full Name
peptidyl-tRNA hydrolase ICT1, mitochondrial isoform 2
NCBI Official Synonym Full Names
mitochondrial ribosomal protein L58
NCBI Official Symbol
MRPL58
NCBI Official Synonym Symbols
DS1; DS-1; ICT1; MRP-L58
UniProt Protein Name
Peptidyl-tRNA hydrolase ICT1, mitochondrial
UniProt Synonym Protein Names
39S ribosomal protein L58, mitochondrial
Protein Family
1-acylglycerol-3-phosphate O-acyltransferase
UniProt Gene Name
MRPL58
UniProt Synonym Gene Names
DS1; ICT1; MRP-L58; DS-1
NCBI Protein Information
peptidyl-tRNA hydrolase ICT1, mitochondrial

NCBI Summary for ICT1

The protein encoded by this gene is a peptidyl-tRNA hydrolase and a vital component of the large mitochondrial ribosome. The encoded protein serves as a ribosome release factor for this ribosome, which translates mitochondrial genes. This protein may be responsible for degrading prematurely-terminated polypeptides and for reusing stalled ribosomes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]
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UniProt Comments for ICT1

Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes.
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Research Articles on ICT1

1. High ICT1 expression is associated with leukemia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ICT1 antibody

 Products by Pathway  Pathway Diagram
 Mitochondrial Translation Pathway antibodies  Mitochondrial Translation Pathway Diagram
 Mitochondrial Translation Elongation Pathway antibodies  Mitochondrial Translation Elongation Pathway Diagram
 Mitochondrial Translation Initiation Pathway antibodies  Mitochondrial Translation Initiation Pathway Diagram
 Mitochondrial Translation Termination Pathway antibodies  Mitochondrial Translation Termination Pathway Diagram
 Organelle Biogenesis And Maintenance Pathway antibodies  Organelle Biogenesis And Maintenance Pathway Diagram

Diseases associated with anti-ICT1 antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >1 publications with ICT1 and Adenocarcinoma
 Atrophy Antibodies  >1 publications with ICT1 and Atrophy

Organs/Tissues associated with anti-ICT1 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >1 publications with ICT1 and Lung
 Liver Antibodies  >1 publications with ICT1 and Liver
 Adipose Tissue Antibodies  >1 publications with ICT1 and Adipose Tissue
 Prostate Antibodies  >1 publications with ICT1 and Prostate
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