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Integrin Alpha 3/ITGA3 (ITGA3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751439

Description: Rabbit IgG polyclonal antibody for Integrin alpha 3 detection. Tested with WB, IHC-P in Human; Mouse; Rat.Background: ITGA3 (INTEGRIN, ALPHA-3), also called CD4 degree C, VLA3 or GAPB3, is a protein that in humans is encoded by the ITGA3 gene. It… More details

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Product Overview

Online Datasheet in PDF
Product Name
Integrin alpha 3/ITGA3, Polyclonal Antibody
Full Product Name
Anti-Integrin alpha 3/ITGA3 Antibody
Product Gene Name
anti-ITGA3 antibody
Product Synonym Names
Integrin alpha-3; CD49 antigen-like family member C; FRP-2; Galactoprotein B3; GAPB3; VLA-3 subunit alpha; CD4 degree C; Integrin alpha-3 heavy chain; Integrin alpha-3 light chain; ITGA3; MSK18; Integrin subunit alpha 3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Molecular Weight
118,756 Da
3D Structure
ModBase 3D Structure for P26006
Immunogen
A synthetic peptide corresponding to a sequence of human Integrin alpha 3 (YSVALHRQTERQQRYLLLAGAPRELAVPDGYTNR).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ITGA3 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-ITGA3 antibody
WB: 0.1-0.5 mug/ml
IHC-P: 0.5-1 mug/ml
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

Western Blot (WB) of anti-ITGA3 antibody

Figure 1. Western blot analysis of Integrin alpha 3 using anti-Integrin alpha 3 antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysate,
Lane 2: human PC-3 whole cell lysate,
Lane 3: human A431 whole cell lysate,
Lane 4: human placenta tissue lysates,
Lane 5: human SHG-44 whole cell lysate,
Lane 6: human U20S whole cell lysate,
Lane 7: human Hela whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin alpha 3 antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Integrin alpha 3 at approximately 130KD. The expected band size for Integrin alpha 3 is at 116KD.
Rabbit Anti-ITGA3 Polyclonal Antibody – Cat# MBS1751439

Immunohistochemistry (IHC) of anti-ITGA3 antibody

Figure 3. IHC analysis of Integrin alpha 3 using anti-Integrin alpha 3 antibody.
Integrin alpha 3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Integrin alpha 3 antibody.
Integrin alpha 3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Integrin alpha 3 antibody.
Integrin alpha 3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Integrin alpha 3 antibody.
Integrin alpha 3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Integrin alpha 3 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-ITGA3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1751439

Product References and Citations for anti-ITGA3 antibody

1. Akula, S. M., Pramod, N. P., Wang, F.-Z., Chandran, B. Integrin alpha-3/beta-1 (CD 4 degree C/29) is a cellular receptor for Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) entry into the target cells. Cell 108: 407-419, 2002. 2. Dulabon, L., Olson, E. C., Taglienti, M. G., Eisenhuth, S., McGrath, B., Walsh, C. A., Kreidberg, J. A., Anton, E. S. Reelin binds alpha-3-beta-1 integrin and inhibits neuronal migration. Neuron 27: 33-44, 2000. 3. Takada Y, Murphy E, Pil P, Chen C, Ginsberg MH, Hemler ME (Oct 1991). "Molecular cloning and expression of the cDNA for alpha 3 subunit of human alpha 3 beta 1 (VLA-3), an integrin receptor for fibronectin, laminin, and collagen".

Other Notes

Small volumes of anti-ITGA3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ITGA3 purchase

MBS1751439 is an antibody targeting ITGA3. Also known as Anti-Integrin alpha 3/ITGA3 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ITGA3. It may not necessarily be applicable to this product.

NCBI GI #
4504747
NCBI GeneID
3675
NCBI Accession #
NP_002195.1
UniProt Primary Accession #
P26006
UniProt Secondary Accession #
A7E246; B7ZM80; B9EGQ1; D3DTX4; D3DTX5
UniProt Related Accession #
P26006
GenBank Nucleotide #
NM_002204.4
NCBI Official Full Name
integrin alpha-3 preproprotein
NCBI Official Synonym Full Names
integrin subunit alpha 3
NCBI Official Symbol
ITGA3
NCBI Official Synonym Symbols
VL3A; CD49C; FRP-2; GAPB3; ILNEB; MSK18; VCA-2; VLA3a; GAP-B3
UniProt Protein Name
Integrin alpha-3
UniProt Synonym Protein Names
CD49 antigen-like family member C; FRP-2; Galactoprotein B3; GAPB3
Protein Family
Integrin
UniProt Gene Name
ITGA3
UniProt Synonym Gene Names
MSK18; GAPB3
NCBI Protein Information
integrin alpha-3

NCBI Summary for ITGA3

The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function as cell surface adhesion molecules. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 3 subunit. This subunit joins with a beta 1 subunit to form an integrin that interacts with extracellular matrix proteins including members of the laminin family. Expression of this gene may be correlated with breast cancer metastasis. [provided by RefSeq, Oct 2015]
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UniProt Comments for ITGA3

Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration.
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Research Articles on ITGA3

1. Bioinformatics analysis identifies ITGA3 as an oncogene in human tongue cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ITGA3 antibody

 Reference Product  PubMed Publications
 ITGB1 antibody  >8 publications with ITGA3 and ITGB1
 ITGB4 antibody  >7 publications with ITGA3 and ITGB4
 ITGB5 antibody  >3 publications with ITGA3 and ITGB5
 LAMA5 antibody  >2 publications with ITGA3 and LAMA5
 FN1 antibody  >2 publications with ITGA3 and FN1
 LAMB3 antibody  >1 publications with ITGA3 and LAMB3
 VTN antibody  >1 publications with ITGA3 and VTN
 LAMA3 antibody  >1 publications with ITGA3 and LAMA3
 RELN antibody  >1 publications with ITGA3 and RELN

Pathways associated with anti-ITGA3 antibody

 Products by Pathway  Pathway Diagram
 Arf6 Trafficking Events Pathway antibodies  Arf6 Trafficking Events Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Basigin Interactions Pathway antibodies  Basigin Interactions Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram

Diseases associated with anti-ITGA3 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >18 publications with ITGA3 and Neoplasms
 Lung Neoplasms Antibodies  >4 publications with ITGA3 and Lung Neoplasms
 Lung Diseases Antibodies  >4 publications with ITGA3 and Lung Diseases
 Nervous System Diseases Antibodies  >3 publications with ITGA3 and Nervous System Diseases
 Congenital Abnormalities Antibodies  >3 publications with ITGA3 and Congenital Abnormalities
 Kidney Diseases Antibodies  >3 publications with ITGA3 and Kidney Diseases
 Breast Neoplasms Antibodies  >2 publications with ITGA3 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >2 publications with ITGA3 and Cell Transformation, Neoplastic
 Neoplasms, Experimental Antibodies  >1 publications with ITGA3 and Neoplasms, Experimental
 Proteinuria Antibodies  >1 publications with ITGA3 and Proteinuria

Organs/Tissues associated with anti-ITGA3 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >6 publications with ITGA3 and Lung
 Kidney Antibodies  >6 publications with ITGA3 and Kidney
 Embryonic Tissue Antibodies  >5 publications with ITGA3 and Embryonic Tissue
 Skin Antibodies  >4 publications with ITGA3 and Skin
 Lymph Node Antibodies  >4 publications with ITGA3 and Lymph Node
 Blood Antibodies  >4 publications with ITGA3 and Blood
 Nerve Antibodies  >3 publications with ITGA3 and Nerve
 Bone Antibodies  >3 publications with ITGA3 and Bone
 Brain Antibodies  >3 publications with ITGA3 and Brain
 Mouth Antibodies  >3 publications with ITGA3 and Mouth
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