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KCC2/SLC12A5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1752513

Description: Rabbit IgG polyclonal antibody for KCC2/SLC12A5 detection. Tested with WB, IHC-P, Direct ELISA in Human; Mouse; Rat.Background: Potassium-chloride transporter member 5 is also known by the names: KCC2 (potassium chloride cotransporter 2) for its… More details

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Product Overview

Online Datasheet in PDF
Product Name
KCC2/SLC12A5, Polyclonal Antibody
Full Product Name
Anti-KCC2/SLC12A5 Antibody
Product Gene Name
anti-KCC2/SLC12A5 antibody
Product Synonym Names
Solute carrier family 12 member 5; Solute carrier family 12 member 5; Electroneutral potassium-chloride cotransporter 2; K-Cl cotransporter 2; hKCC2; Neuronal K-Cl cotransporter; SLC12A5; KCC2; KIAA1176
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
OMIM
606726
3D Structure
ModBase 3D Structure for Q9H2X9
Immunogen
E Coli-derived human KCC2/SLC12A5 recombinant protein (Position: A228-N833).
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Relevant Detection Systems
We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC(P).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500mug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-KCC2/SLC12A5 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)
Application Notes for anti-KCC2/SLC12A5 antibody
WB: 0.1-0.5ug/ml
IHC-P: 0.5-1ug/ml
Direct ELISA: 0.1-0.5ug/ml

Product References and Citations for anti-KCC2/SLC12A5 antibody

1. Sallinen, R., Tornberg, J., Putkiranta, M., Horelli-Kuitunen, N., Airaksinen, M. S., Wessman, M. Chromosomal localization of SLC12A5/Slc12a5, the human and mouse genes for the neuron-specific K(+)-Cl(-) cotransporter (KCC2) defines a new region of conserved homology. Cytogenet. Cell Genet. 94: 67-70, 2001. Note: Erratum: Cytogenet. Genome Res. 98: 232 only, 2002. 2. Stodberg, T., McTague, A., Ruiz, A. J., Hirata, H., Zhen, J., Long, P., Farabella, I., Meyer, E., Kawahara, A., Vassallo, G., Stivaros, S. M., Bjursell, M. K. Mutations in SLC12A5 in epilepsy of infancy with migrating focal seizures. Nature Commun. 6: 8038, 2015. Note: Electronic Article.

Other Notes

Small volumes of anti-KCC2/SLC12A5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KCC2/SLC12A5 purchase

MBS1752513 is an antibody targeting KCC2/SLC12A5. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KCC2/SLC12A5. It may not necessarily be applicable to this product.

NCBI GI #
161784306
NCBI GeneID
57468
NCBI Accession #
Q9H2X9.3
UniProt Primary Accession #
Q9H2X9
UniProt Related Accession #
Q9H2X9
NCBI Official Full Name
Solute carrier family 12 member 5
NCBI Official Synonym Full Names
solute carrier family 12 member 5
NCBI Official Symbol
SLC12A5
NCBI Official Synonym Symbols
KCC2; EIG14; hKCC2; EIEE34
UniProt Protein Name
Solute carrier family 12 member 5
UniProt Synonym Protein Names
Electroneutral potassium-chloride cotransporter 2; K-Cl cotransporter 2; hKCC2; Neuronal K-Cl cotransporter
UniProt Gene Name
SLC12A5
UniProt Synonym Gene Names
KCC2; KIAA1176; hKCC2
UniProt Entry Name
S12A5_HUMAN
NCBI Protein Information
solute carrier family 12 member 5

NCBI Summary for KCC2/SLC12A5

K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2008]
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UniProt Comments for KCC2/SLC12A5

KCC2: Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons. Homomultimer and heteromultimer with other K-Cl cotransporters. Interacts with AP2A1. Brain specific. Detected in neuronal cells. Inhibited by WNK3. Belongs to the SLC12A transporter family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, SLC family; Membrane protein, multi-pass; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: cell soma; integral to plasma membrane; integral to membrane; plasma membrane; dendritic shaft

Molecular Function: protein kinase binding; potassium:chloride symporter activity

Biological Process: response to drug; synaptic transmission; transport; cellular ion homeostasis; multicellular organism growth; ion transport; learning; transmembrane transport; potassium ion transport; thermosensory behavior
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Research Articles on KCC2/SLC12A5

1. Study significantly lower NKCC1 DNA methylation and significantly higher KCC2 DNA methylation levels in patients with juvenile myoclonic epilepsy (JME) compared with the healthy controls. This implies that NKCC1 expression can be higher and KCC2 expression can be reduced in affected people.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-KCC2/SLC12A5 antibody

 Products by Pathway  Pathway Diagram
 Cation-coupled Chloride Cotransporters Pathway antibodies  Cation-coupled Chloride Cotransporters Pathway Diagram
 GABAergic Synapse Pathway antibodies  GABAergic Synapse Pathway Diagram
 GABAergic Synapse Pathway antibodies  GABAergic Synapse Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway antibodies  Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway Diagram

Diseases associated with anti-KCC2/SLC12A5 antibody

 Disease Name  Pubmed Publications
 Epilepsy Antibodies  >3 publications with KCC2/SLC12A5 and Epilepsy
 Growth Disorders Antibodies  >2 publications with KCC2/SLC12A5 and Growth Disorders
 Cognition Disorders Antibodies  >2 publications with KCC2/SLC12A5 and Cognition Disorders
 Seizures Antibodies  >2 publications with KCC2/SLC12A5 and Seizures
 Cardiovascular Diseases Antibodies  >1 publications with KCC2/SLC12A5 and Cardiovascular Diseases
 Liver Diseases Antibodies  >1 publications with KCC2/SLC12A5 and Liver Diseases
 Hypertension Antibodies  >1 publications with KCC2/SLC12A5 and Hypertension
 Kidney Diseases Antibodies  >1 publications with KCC2/SLC12A5 and Kidney Diseases
 Necrosis Antibodies  >1 publications with KCC2/SLC12A5 and Necrosis

Organs/Tissues associated with anti-KCC2/SLC12A5 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >9 publications with KCC2/SLC12A5 and Brain
 Kidney Antibodies  >3 publications with KCC2/SLC12A5 and Kidney
 Muscle Antibodies  >1 publications with KCC2/SLC12A5 and Muscle
 Lung Antibodies  >1 publications with KCC2/SLC12A5 and Lung
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