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KNG1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9607200

Function: (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin-and… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
KNG1, Polyclonal Antibody
Full Product Name
KNG1 Antibody
Product Gene Name
anti-KNG1 antibody
Product Synonym Names
Alpha-2-thiol proteinase inhibitor; BDK; BK; Bradykinin; Bradykinin included; Fitzgerald factor; FLAUJEAC FACTOR; High molecular weight kininogen; HMWK; Ile-Ser-Bradykinin; Kallidin I; Kallidin II; KNG; KNG1; KNG1_HUMAN; Low molecular weight growth-promoting factor; WILLIAMS FACTOR; Williams-Fitzgerald-Flaujeac factor
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
KNG1 antibody detects endogenous levels of total KNG1
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
KNG1 peptide (MBS9625916) is used for blocking the activity of KNG1 antibody (MBS9607200)
Molecular Weight
Observed: 72 kDa
Predicted: 72 kDa
3D Structure
ModBase 3D Structure for P01042
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human KNG1
Subcellular Location
Secreted > Extracellular Space.
Tissue Specificity
Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-KNG1 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-KNG1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-KNG1 antibody

MBS9607200 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Rabbit Anti-KNG1 Polyclonal Antibody – Cat# MBS9607200

Western Blot (WB) of anti-KNG1 antibody

Western blot analysis of extracts from 293, using KNG1 Antibody. Lane 1 was treated with the antigen-specific peptide.
Rabbit Anti-KNG1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9607200

Immunohistochemistry (IHC) of anti-KNG1 antibody

MBS9607200 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-KNG1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9607200

Immunofluorescence (IF) of anti-KNG1 antibody

MBS9607200 staining HepG2  cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Rabbit Anti-KNG1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9607200

Western Blot (WB) of anti-KNG1 antibody

Western blot analysis of Mouse liver tissue lysates, using KNG1 Antibody. The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-KNG1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9607200

Other Notes

Small volumes of anti-KNG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KNG1 purchase

MBS9607200 is an antibody targeting KNG1. Also known as KNG1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KNG1. It may not necessarily be applicable to this product.

NCBI GI #
4504893
NCBI GeneID
3827
NCBI Accession #
NP_000884.1
UniProt Primary Accession #
P01042
UniProt Secondary Accession #
P01043; Q53EQ0; Q6PAU9; Q7M4P1; A8K474; B2RCR2; C9JEX1
UniProt Related Accession #
P01042
GenBank Nucleotide #
NM_000893.4
NCBI Official Full Name
kininogen-1 isoform 2
NCBI Official Synonym Full Names
kininogen 1
NCBI Official Symbol
KNG1
NCBI Official Synonym Symbols
BK; BDK; KNG; HMWK
UniProt Protein Name
Kininogen-1
UniProt Synonym Protein Names
Alpha-2-thiol proteinase inhibitor; Fitzgerald factor; High molecular weight kininogen; HMWK
Protein Family
Kininogen
UniProt Gene Name
KNG1
UniProt Synonym Gene Names
BDK; KNG; HMWK
NCBI Protein Information
kininogen-1

NCBI Summary for KNG1

This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. Bradykinin also functions as an antimicrobial peptide with antibacterial and antifungal activity. In contrast to HMWK, LMWK is not involved in blood coagulation. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2014]
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UniProt Comments for KNG1

1 Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.
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Research Articles on KNG1

1. Kininogen binds to the cell surface-exposed proteins of Candida parapsilosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KNG1 antibody

 Reference Product  PubMed Publications
 F11 antibody  >3 publications with KNG1 and F11
 KLKB1 antibody  >1 publications with KNG1 and KLKB1
 HABP2 antibody  >1 publications with KNG1 and HABP2
 AGT antibody  >1 publications with KNG1 and AGT

Pathways associated with anti-KNG1 antibody

 Products by Pathway  Pathway Diagram
 ACE Inhibitor Pathway antibodies  ACE Inhibitor Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram

Diseases associated with anti-KNG1 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >8 publications with KNG1 and Cardiovascular Diseases
 Thrombosis Antibodies  >4 publications with KNG1 and Thrombosis
 Heart Diseases Antibodies  >4 publications with KNG1 and Heart Diseases
 Atherosclerosis Antibodies  >2 publications with KNG1 and Atherosclerosis
 Kidney Diseases Antibodies  >1 publications with KNG1 and Kidney Diseases
 Inflammation Antibodies  >1 publications with KNG1 and Inflammation
 Arrhythmias, Cardiac Antibodies  >1 publications with KNG1 and Arrhythmias, Cardiac
 Edema Antibodies  >1 publications with KNG1 and Edema

Organs/Tissues associated with anti-KNG1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1 publications with KNG1 and Brain
 Liver Antibodies  >1 publications with KNG1 and Liver
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