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KRAS Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9210256

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results… More details

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Product Overview

Online Datasheet in PDF
Product Name
KRAS, Polyclonal Antibody
Full Product Name
KRAS Antibody (C-term)
Product Gene Name
anti-KRAS antibody
Product Synonym Names
GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; GTPase KRas; N-terminally processed; KRAS; KRAS2; RASK2
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat (Predicted: Rat)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Specificity
This KRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-174 amino acids from the C-terminal region of human KRAS.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
KRAS peptide (MBS9221647) is used for blocking the activity of KRAS antibody (MBS9210256)
Immunogen Sequence Positions
146-174
OMIM
190070
3D Structure
ModBase 3D Structure for P01116
Function
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:20949621). Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed:24623306).
Cellular Location
Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytosol

Product Categories/Family for anti-KRAS antibody

Cancer; Signal Transduction
Applications Tested/Suitable for anti-KRAS antibody
Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Flow Cytometry (FC)
Application Notes for anti-KRAS antibody
WB~~1:2000
IF~~1:10~50
FC~~1:10~50

Western Blot (WB) of anti-KRAS antibody

Anti-KRAS Antibody (C-term) at 1:2000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 22 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-KRAS Polyclonal Antibody – Cat# MBS9210256

Western Blot (WB) of anti-KRAS antibody

All lanes : Anti-KRAS Antibody (C-term) at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: 293T/17 whole cell lysate
Lane 3: C2C12 whole cell lysate
Lane 4: C6 whole cell lysate
Lane 5: HT-29 whole cell lysate
Lane 6: PC-12 whole cell lysate Lysates/proteins at 20 ug per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 22 kDa
Rabbit Anti-KRAS Polyclonal Antibody, Western Blot (WB) – Cat# MBS9210256

Western Blot (WB) of anti-KRAS antibody

All lanes : Anti-KRAS Antibody (C-term) at 1:2000 dilution
Lane 1: 293T/17 whole cell lysate
Lane 2: C2C12 whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: HT-29 whole cell lysate
Lane 5: K562 whole cell lysate Lane 5: PC-12 whole cell lysate
Lane 5: Ramos whole cell lysate Lysates/proteins at 20 ug per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 22 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-KRAS Polyclonal Antibody, Western Blot (WB) – Cat# MBS9210256

Immunofluorescence (IF) of anti-KRAS antibody

Confocal immunofluorescent analysis of KRAS Antibody (C-term)(MBS9210256) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-KRAS Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9210256

Flow Cytometry (FC/FACS) of anti-KRAS antibody

KRAS Antibody (C-term)(MBS9210256) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-KRAS Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS9210256

Product References and Citations for anti-KRAS antibody

Bruckman, K.C., et al. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 110(5):632-637(2010)
Wong, K.K., et al. Am. J. Pathol. 177(4):1611-1617(2010)
Irahara, N., et al. Diagn. Mol. Pathol. 19(3):157-163(2010)
Carotenuto, P., et al. Pharmacogenomics 11(8):1169-1179(2010)
Leventopoulos, G., et al. Clin. Exp. Rheumatol. 28(4):556-557(2010)

Other Notes

Small volumes of anti-KRAS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KRAS purchase

MBS9210256 is an antibody targeting KRAS. Also known as KRAS Antibody (C-term). Reactive in Human, Mouse, Rat (Predicted: Rat). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KRAS. It may not necessarily be applicable to this product.

NCBI GI #
15718761
NCBI GeneID
3845
NCBI Accession #
NP_004976.2
NCBI Related Accession #
NP_203524.1
UniProt Primary Accession #
P01116
UniProt Secondary Accession #
P01118; Q96D10; A8K8Z5; B0LPF9
UniProt Related Accession #
P01116
GenBank Nucleotide #
NM_004985.4
NCBI Official Full Name
GTPase KRas isoform b
NCBI Official Synonym Full Names
Kirsten rat sarcoma viral oncogene homolog
NCBI Official Symbol
KRAS
NCBI Official Synonym Symbols
NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
UniProt Protein Name
GTPase KRas
UniProt Synonym Protein Names
K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras
Protein Family
GTPase
UniProt Gene Name
KRAS
UniProt Synonym Gene Names
KRAS2; RASK2
UniProt Entry Name
RASK_HUMAN
NCBI Protein Information
GTPase KRas

UniProt Comments for KRAS

KRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts with PHLPP. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein; Motility/polarity/chemotaxis; G protein, monomeric; G protein, monomeric, Ras; Oncoprotein

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: extrinsic to internal side of plasma membrane; focal adhesion; membrane; mitochondrion; cytoplasm; plasma membrane; cytosol; lipid raft

Molecular Function: protein binding; GDP binding; GTP binding; GMP binding; protein complex binding; LRR domain binding

Biological Process: regulation of long-term neuronal synaptic plasticity; axon guidance; nerve growth factor receptor signaling pathway; activation of MAPKK activity; response to glucocorticoid stimulus; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; small GTPase mediated signal transduction; positive regulation of cell proliferation; visual learning; negative regulation of neuron apoptosis; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; positive regulation of nitric-oxide synthase activity; MAPKKK cascade; social behavior; response to mineralocorticoid stimulus; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; Ras protein signal transduction; insulin receptor signaling pathway; striated muscle cell differentiation; innate immune response; positive regulation of protein amino acid phosphorylation; blood coagulation; vascular endothelial growth factor receptor signaling pathway; actin cytoskeleton organization and biogenesis; leukocyte migration

Disease: Bladder Cancer; Gastric Cancer, Hereditary Diffuse; Pancreatic Cancer; Noonan Syndrome 3; Leukemia, Acute Myeloid; Breast Cancer; Lung Cancer; Schimmelpenning-feuerstein-mims Syndrome; Cardiofaciocutaneous Syndrome 2
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Research Articles on KRAS

1. Targeted sequencing on primary, metastatic, and normal tissue from colorectal cancer patients found a high degree of concordance for KRAS, NRAS, and BRAF mutations that were identical in both the primary and metastatic tumors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KRAS antibody

 Reference Product  PubMed Publications
 EGFR antibody  >2073 publications with KRAS and EGFR
 PIK3CA antibody  >741 publications with KRAS and PIK3CA
 TP53 antibody  >595 publications with KRAS and TP53
 PTPN11 antibody  >148 publications with KRAS and PTPN11
 MAP2K1 antibody  >117 publications with KRAS and MAP2K1
 SOS1 antibody  >101 publications with KRAS and SOS1
 RAF1 antibody  >85 publications with KRAS and RAF1
 RALGDS antibody  >38 publications with KRAS and RALGDS
 BCL2 antibody  >32 publications with KRAS and BCL2
 ARAF antibody  >6 publications with KRAS and ARAF

Pathways associated with anti-KRAS antibody

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Diseases associated with anti-KRAS antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >3152 publications with KRAS and Carcinoma
 Colorectal Neoplasms Antibodies  >2242 publications with KRAS and Colorectal Neoplasms
 Adenocarcinoma Antibodies  >2065 publications with KRAS and Adenocarcinoma
 Lung Neoplasms Antibodies  >1484 publications with KRAS and Lung Neoplasms
 Cell Transformation, Neoplastic Antibodies  >952 publications with KRAS and Cell Transformation, Neoplastic
 Pancreatic Neoplasms Antibodies  >764 publications with KRAS and Pancreatic Neoplasms
 Carcinoma, Non-Small-Cell Lung Antibodies  >726 publications with KRAS and Carcinoma, Non-Small-Cell Lung
 Neoplasm Metastasis Antibodies  >716 publications with KRAS and Neoplasm Metastasis
 Neoplasms, Experimental Antibodies  >541 publications with KRAS and Neoplasms, Experimental
 Colonic Neoplasms Antibodies  >491 publications with KRAS and Colonic Neoplasms

Organs/Tissues associated with anti-KRAS antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >1891 publications with KRAS and Lung
 Blood Antibodies  >867 publications with KRAS and Blood
 Liver Antibodies  >569 publications with KRAS and Liver
 Skin Antibodies  >392 publications with KRAS and Skin
 Vascular Antibodies  >375 publications with KRAS and Vascular
 Pancreas Antibodies  >368 publications with KRAS and Pancreas
 Brain Antibodies  >294 publications with KRAS and Brain
 Lymph Node Antibodies  >237 publications with KRAS and Lymph Node
 Lymph Antibodies  >237 publications with KRAS and Lymph
 Bone Antibodies  >208 publications with KRAS and Bone
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