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LIG4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9607244

Description: Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an… More details

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Product Overview

Online Datasheet in PDF
Product Name
LIG4, Polyclonal Antibody
Full Product Name
LIG4 Antibody
Product Gene Name
anti-LIG4 antibody
Product Synonym Names
DNA ligase IV ATP dependent; DNA joinase; DNA ligase 4; DNA ligase IV; DNA repair enzyme; DNLI4_HUMAN; LIG 4; LIG4; LIG4S; Ligase IV; Ligase IV DNA ATP dependent; Polydeoxyribonucleotide synthase [ATP] 4; Polydeoxyribonucleotide synthase 4; Polydeoxyribonucleotide synthase; Polynucleotide ligase; Sealase
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
LIG4 antibody detects endogenous levels of total LIG4
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
DNA Ligase IV peptide (MBS9625960) is used for blocking the activity of DNA Ligase IV antibody (MBS9607244)
Molecular Weight
Observed: 104 kDa
Predicted: 104 kDa
3D Structure
ModBase 3D Structure for P49917
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human LIG4
Subcellular Location
Nucleus.
Tissue Specificity
Testis, thymus, prostate and heart.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (82%), Zebrafish (82%), Bovine (82%), Horse (100%), Rabbit (91%), Dog (100%), Chicken (100%), Xenopus (91%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-LIG4 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-LIG4 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500

Western Blot (WB) of anti-LIG4 antibody

Western blot analysis of extracts from Hela, using LIG4 Antibody. Lane 1 was treated with the antigen-specific peptide.
Rabbit Anti-LIG4 Polyclonal Antibody – Cat# MBS9607244

Western Blot (WB) of anti-LIG4 antibody

Western blot analysis of Mouse heart lysate, using LIG4 Antibody. The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-LIG4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9607244

Immunohistochemistry (IHC) of anti-LIG4 antibody

MBS9607244 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-LIG4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9607244

Immunofluorescence (IF) of anti-LIG4 antibody

MBS9607244 staining HepG2  cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Rabbit Anti-LIG4 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9607244

Other Notes

Small volumes of anti-LIG4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LIG4 purchase

MBS9607244 is an antibody targeting LIG4. Also known as LIG4 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LIG4. It may not necessarily be applicable to this product.

NCBI GI #
148539894
NCBI GeneID
3981
NCBI Accession #
NP_001091738.1
UniProt Primary Accession #
P49917
UniProt Secondary Accession #
Q8IY66; Q8TEU5
UniProt Related Accession #
P49917
GenBank Nucleotide #
NM_001098268.1
NCBI Official Full Name
DNA ligase 4 isoform 1
NCBI Official Synonym Full Names
DNA ligase 4
NCBI Official Symbol
LIG4
NCBI Official Synonym Symbols
LIG4S
UniProt Protein Name
DNA ligase 4
UniProt Synonym Protein Names
DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4
Protein Family
DNA ligase
UniProt Gene Name
LIG4
NCBI Protein Information
DNA ligase 4

NCBI Summary for LIG4

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
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UniProt Comments for LIG4

Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
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Research Articles on LIG4

1. study demonstrated that there was an association between DNA ligase 4 Thr9Ile polymorphism and male infertility and suggests CT genotype as a risk factor for male infertility

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LIG4 antibody

 Reference Product  PubMed Publications
 XRCC4 antibody  >41 publications with LIG4 and XRCC4
 PRKDC antibody  >12 publications with LIG4 and PRKDC
 NHEJ1 antibody  >5 publications with LIG4 and NHEJ1
 APLF antibody  >1 publications with LIG4 and APLF

Pathways associated with anti-LIG4 antibody

 Products by Pathway  Pathway Diagram
 2-LTR Circle Formation Pathway antibodies  2-LTR Circle Formation Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Double-Strand Break Repair Pathway antibodies  Double-Strand Break Repair Pathway Diagram
 Early Phase Of HIV Life Cycle Pathway antibodies  Early Phase Of HIV Life Cycle Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 HIV Life Cycle Pathway antibodies  HIV Life Cycle Pathway Diagram
 Integration Of Provirus Pathway antibodies  Integration Of Provirus Pathway Diagram
 Non-homologous End-joining Pathway antibodies  Non-homologous End-joining Pathway Diagram
 Non-homologous End-joining Pathway antibodies  Non-homologous End-joining Pathway Diagram

Diseases associated with anti-LIG4 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >42 publications with LIG4 and Neoplasms
 LIG4 Syndrome Antibodies  >27 publications with LIG4 and LIG4 Syndrome
 Nervous System Diseases Antibodies  >10 publications with LIG4 and Nervous System Diseases
 Lymphoma Antibodies  >9 publications with LIG4 and Lymphoma
 Respiratory Tract Diseases Antibodies  >6 publications with LIG4 and Respiratory Tract Diseases
 Neoplasms, Experimental Antibodies  >6 publications with LIG4 and Neoplasms, Experimental
 Drug Toxicity Antibodies  >5 publications with LIG4 and Drug Toxicity
 Carcinoma, Squamous Cell Antibodies  >5 publications with LIG4 and Carcinoma, Squamous Cell
 Lung Neoplasms Antibodies  >4 publications with LIG4 and Lung Neoplasms
 Disease Models, Animal Antibodies  >4 publications with LIG4 and Disease Models, Animal

Organs/Tissues associated with anti-LIG4 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >25 publications with LIG4 and Blood
 Brain Antibodies  >8 publications with LIG4 and Brain
 Bone Antibodies  >7 publications with LIG4 and Bone
 Skin Antibodies  >4 publications with LIG4 and Skin
 Prostate Antibodies  >3 publications with LIG4 and Prostate
 Muscle Antibodies  >2 publications with LIG4 and Muscle
 Pharynx Antibodies  >2 publications with LIG4 and Pharynx
 Thymus Antibodies  >2 publications with LIG4 and Thymus
 Kidney Antibodies  >1 publications with LIG4 and Kidney
 Testis Antibodies  >1 publications with LIG4 and Testis
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