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MAP1S Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758475

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MAP1S, Polyclonal Antibody
Full Product Name
Anti-MAP1S Antibody Picoband
Product Gene Name
anti-MAP1S antibody
Product Synonym Names
interleukin 3 receptor subunit alpha; Interleukin-3 receptor subunit alpha; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA; CD123; IL3RA; IL3R
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Brain.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
1059
OMIM
607573
3D Structure
ModBase 3D Structure for Q66K74
Immunogen
E coli-derived human MAP1S recombinant protein (Position: D45-H1021).
Subcellular Localization
Membrane. Single-pass type I membrane protein.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
This is a receptor for interleukin-3.

Product Categories/Family for anti-MAP1S antibody

Primary Antibodies; Adaptive Immunity; B Cells; Hematopoietic Progenitors; Immunoglobulins; Immunology; Lymphoid; Receptors; Stem Cells
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-MAP1S antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-MAP1S antibody
WB: 0.25-0.5ug/ml, Human
IHC: 2-5ug/ml, Mouse, Rat
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-MAP1S antibody

Figure 1. Western blot analysis of MAP1S using anti-MAP1S antibody (MBS1758475).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP1S antigen affinity purified polyclonal antibody (#MBS1758475) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for MAP1S at approximately 150 kDa. The expected band size for MAP1S is at 112 kDa.
Rabbit Anti-MAP1S Polyclonal Antibody – Cat# MBS1758475

Immunohistochemistry (IHC) of anti-MAP1S antibody

Figure 2. IHC analysis of MAP1S using anti-MAP1S antibody (MBS1758475).
MAP1S was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MAP1S Antibody (MBS1758475) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-MAP1S Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1758475

Immunohistochemistry (IHC) of anti-MAP1S antibody

Figure 3. IHC analysis of MAP1S using anti-MAP1S antibody (MBS1758475).
MAP1S was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MAP1S Antibody (MBS1758475) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-MAP1S Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1758475

Flow Cytometry (FC/FACS) of anti-MAP1S antibody

Figure 4. Flow Cytometry analysis of PC-3 cells using anti-MAP1S antibody (MBS1758475).
Overlay histogram showing PC-3 cells stained with MBS1758475 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAP1S Antibody (MBS1758475, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-MAP1S Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758475

Product References and Citations for anti-MAP1S antibody

1. Gross, M. B. Personal Communication. Baltimore, Md. 4/10/2014.2. Liu, L., McKeehan, W. L. Sequence analysis of LRPPRC and its SEC1 domain interaction partners suggest roles in cytoskeletal organization, vesicular trafficking, nucleocytosolic shuttling, and chromosome activity. Genomics 79: 124-136, 2002. 3. Wang, W., Lundin, V. F., Millan, I., Zeng, A., Chen, X., Yang, J., Allen, E., Chen, N., Bach, G., Hsu, A., Maloney, M. T., Kapur, M., Yang, Y. Nemitin, a novel Map8/Map1s interacting protein with Wd40 repeats. PLoS ONE 7: e33094, 2012. Note: Electronic Article.

Other Notes

Small volumes of anti-MAP1S antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAP1S purchase

MBS1758475 is an antibody targeting MAP1S. Also known as Anti-MAP1S Antibody Picoband. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MAP1S. It may not necessarily be applicable to this product.

NCBI GI #
160410004
NCBI GeneID
55201
NCBI Accession #
Q66K74.2
UniProt Primary Accession #
Q66K74
UniProt Secondary Accession #
Q27QB1; Q6NXF1; Q8N3L8; Q8N3W5; Q8NI88; Q96H94; Q96IT4; Q96SP8; Q9BRC6; Q9H928; B4DH53
UniProt Related Accession #
Q66K74
NCBI Official Full Name
Microtubule-associated protein 1S
NCBI Official Synonym Full Names
microtubule-associated protein 1S
NCBI Official Symbol
MAP1S
NCBI Official Synonym Symbols
MAP8; BPY2IP1; C19orf5; VCY2IP1; VCY2IP-1
UniProt Protein Name
Microtubule-associated protein 1S
UniProt Synonym Protein Names
BPY2-interacting protein 1; Microtubule-associated protein 8; Variable charge Y chromosome 2-interacting protein 1; VCY2-interacting protein 1; VCY2IP-1
Protein Family
Microtubule-associated protein
UniProt Gene Name
MAP1S
UniProt Synonym Gene Names
BPY2IP1; C19orf5; MAP8; VCY2IP1; MAP-1S; VCY2-interacting protein 1; VCY2IP-1
UniProt Entry Name
MAP1S_HUMAN
NCBI Protein Information
microtubule-associated protein 1S; MAP-1S; BPY2 interacting protein 1; BPY2-interacting protein 1; VCY2 interacting protein 1; VCY2-interacting protein 1; microtubule-associated protein 8; variable charge Y chromosome 2-interacting protein 1

UniProt Comments for MAP1S

BPY2-IP1: Microtubule-associated protein that mediates aggregation of mitochondria resulting in cell death and genomic destruction (MAGD). Plays a role in anchoring the microtubule-organizing center to the centrosomes. Binds to DNA. Plays a role in apoptosis. Involved in the formation of microtubule bundles. Belongs to the MAP1 family.

Protein type: Microtubule-binding; Apoptosis

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: microtubule; cell projection; cell soma; perinuclear region of cytoplasm; cytoplasm; dendrite; nucleolus; spindle; synapse; nucleus; cytosol; cell junction

Molecular Function: tubulin binding; actin filament binding; identical protein binding; protein binding; DNA binding; microtubule binding; deoxyribonuclease activity; beta-tubulin binding

Biological Process: nervous system development; brain development; DNA fragmentation during apoptosis; neurite morphogenesis; microtubule bundle formation; mitochondrion transport along microtubule
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Research Articles on MAP1S

1. The PU.1-regulated MAP1S gene is implicated in neutrophil differentiation and autophagy control.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-MAP1S antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >2 publications with MAP1S and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >2 publications with MAP1S and Cell Transformation, Neoplastic
 Lymphoma Antibodies  >1 publications with MAP1S and Lymphoma
 Stomach Neoplasms Antibodies  >1 publications with MAP1S and Stomach Neoplasms
 Immune System Diseases Antibodies  >1 publications with MAP1S and Immune System Diseases
 Aneuploidy Antibodies  >1 publications with MAP1S and Aneuploidy
 Hyperplasia Antibodies  >1 publications with MAP1S and Hyperplasia
 Liver Neoplasms Antibodies  >1 publications with MAP1S and Liver Neoplasms
 Liver Diseases Antibodies  >1 publications with MAP1S and Liver Diseases

Organs/Tissues associated with anti-MAP1S antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >5 publications with MAP1S and Nerve
 Brain Antibodies  >5 publications with MAP1S and Brain
 Prostate Antibodies  >2 publications with MAP1S and Prostate
 Liver Antibodies  >2 publications with MAP1S and Liver
 Bone Antibodies  >1 publications with MAP1S and Bone
 Muscle Antibodies  >1 publications with MAP1S and Muscle
 Eye Antibodies  >1 publications with MAP1S and Eye
 Uterus Antibodies  >1 publications with MAP1S and Uterus
 Embryonic Tissue Antibodies  >1 publications with MAP1S and Embryonic Tissue
 Lung Antibodies  >1 publications with MAP1S and Lung
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