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MAST4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9233328

MBS9233328 is an antibody targeting MAST4. Reactive in Human, Mouse, Rat. Host: Rabbit. Rabbit polyclonal antibody to MAST4 More details

$475 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MAST4, Polyclonal Antibody
Full Product Name
Anti-MAST4 Antibody
Product Gene Name
anti-MAST4 antibody
Product Synonym Names
KIAA0303; Microtubule-associated serine/threonine-protein kinase 4
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight
284,378 Da
Chromosome Location
Chromosome: 5; NC_000005.9 (65892176..66465423). Location: 5q12.3
3D Structure
ModBase 3D Structure for O15021
Applications Tested/Suitable for anti-MAST4 antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Wester Blot (WB) of anti-MAST4 antibody

Dilution: WB 1/500 - 1/1000
Western blot analysis of MAST4 expression in HeLa (A), HuvEc (B) whole cell lysates.
Rabbit Anti-MAST4 Polyclonal Antibody – Cat# MBS9233328

Immunohistochemistry (IHC) of anti-MAST4 antibody

Dilution: IH 1/50 - 1/200
Immunohistochemical analysis of MAST4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-MAST4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9233328

Immunofluorescence (IF) of anti-MAST4 antibody

Dilution: IF: 1/50 - 1/200
Immunofluorescent analysis of MAST4 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-MAST4 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9233328

Other Notes

Small volumes of anti-MAST4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MAST4. It may not necessarily be applicable to this product.

NCBI GI #
148727255
NCBI GeneID
375449
NCBI Accession #
NP_055998.1
UniProt Primary Accession #
O15021
UniProt Secondary Accession #
Q05EE6; Q6ZN07; Q8N4X4; Q96LY3; A6NL49; B5ME48
UniProt Related Accession #
O15021
GenBank Nucleotide #
NM_015183.2
NCBI Official Full Name
microtubule-associated serine/threonine-protein kinase 4 isoform a
NCBI Official Synonym Full Names
microtubule associated serine/threonine kinase family member 4
NCBI Official Symbol
MAST4
UniProt Protein Name
Microtubule-associated serine/threonine-protein kinase 4
Protein Family
Microtubule-associated serine/threonine-protein kinase
UniProt Gene Name
MAST4
UniProt Synonym Gene Names
KIAA0303
UniProt Entry Name
MAST4_HUMAN
NCBI Protein Information
microtubule-associated serine/threonine-protein kinase 4

NCBI Summary for MAST4

This gene encodes a member of the microtubule-associated serine/threonine protein kinases. The proteins in this family contain a domain that gives the kinase the ability to determine its own scaffold to control the effects of their kinase activities. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014]
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UniProt Comments for MAST4

MAST4: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; AGC group; MAST family

Chromosomal Location of Human Ortholog: 5q12.3

Cellular Component: cytoplasm

Molecular Function: protein serine/threonine kinase activity; magnesium ion binding; ATP binding

Biological Process: protein amino acid phosphorylation
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Research Articles on MAST4

1. A protein encoded by this locus was found to be differentially expressed in postmortem brains from patients with atypical frontotemporal lobar degeneration.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-MAST4 antibody

 Disease Name  Pubmed Publications
 Lung Neoplasms Antibodies  >1 publications with MAST4 and Lung Neoplasms
 Neoplasms Antibodies  >1 publications with MAST4 and Neoplasms

Organs/Tissues associated with anti-MAST4 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >2 publications with MAST4 and Testis
 Bone Antibodies  >1 publications with MAST4 and Bone
 Intestine Antibodies  >1 publications with MAST4 and Intestine
 Blood Antibodies  >1 publications with MAST4 and Blood
 Prostate Antibodies  >1 publications with MAST4 and Prostate
 Muscle Antibodies  >1 publications with MAST4 and Muscle
 Heart Antibodies  >1 publications with MAST4 and Heart
 Lung Antibodies  >1 publications with MAST4 and Lung
 Brain Antibodies  >1 publications with MAST4 and Brain
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