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MED22 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9609931

Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA… More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MED22, Polyclonal Antibody
Full Product Name
MED22 Antibody
Product Gene Name
anti-MED22 antibody
Product Synonym Names
MED22; MED22_HUMAN; MED24; Mediator complex subunit 22; Mediator of RNA polymerase II transcription subunit 22; OTTHUMP00000022480; OTTMUSP00000012503; RGD1564893; Surf 5; Surf-5; Surfeit 5; Surfeit locus protein 5
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
MED22 antibody detects endogenous levels of total MED22
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
MED22 peptide (MBS9628635) is used for blocking the activity of MED22 antibody (MBS9609931)
Molecular Weight
Observed: 22 kDa
Predicted: 23 kDa
3D Structure
ModBase 3D Structure for Q15528
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human MED22
Subcellular Location
Nucleus.
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MED22 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-MED22 antibody
WB: 1:1000-3000
IHC: 1:200

Western Blot (WB) of anti-MED22 antibody

Western blot analysis of MED22 using NIH-3T3 whole cell lysates
Rabbit Anti-MED22 Polyclonal Antibody – Cat# MBS9609931

Immunohistochemistry (IHC) of anti-MED22 antibody

MBS9609931 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-MED22 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9609931

Other Notes

Small volumes of anti-MED22 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MED22 purchase

MBS9609931 is an antibody targeting MED22. Also known as MED22 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MED22. It may not necessarily be applicable to this product.

NCBI GI #
19557695
NCBI GeneID
6837
NCBI Accession #
NP_598395.1
UniProt Primary Accession #
Q15528
UniProt Secondary Accession #
O76072; Q5T8U0; B3KW83; B3KWX4
UniProt Related Accession #
Q15528
GenBank Nucleotide #
NM_133640.4
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 22 isoform b
NCBI Official Synonym Full Names
mediator complex subunit 22
NCBI Official Symbol
MED22
NCBI Official Synonym Symbols
SRB6; MED24; SURF5; surf-5
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 22
UniProt Synonym Protein Names
Mediator complex subunit 22; Surfeit locus protein 5; Surf-5
Protein Family
Mediator of RNA polymerase II transcription
UniProt Gene Name
MED22
UniProt Synonym Gene Names
SURF5; Surf-5
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 22

NCBI Summary for MED22

This gene encodes a protein component of the mediator complex, which functions in the regulation of transcription by bridging interactions between gene-specific regulatory factors, RNA polymerase II, and general transcription factors. Alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq, Jul 2013]
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UniProt Comments for MED22

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
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Research Articles on MED22

1. functional communications between the MED1 subunit and the MED24-containing submodule that mediate estrogen receptor functions and growth of both normal mammary epithelial cells and breast carcinoma cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MED22 antibody

 Reference Product  PubMed Publications
 MED17 antibody  >2 publications with MED22 and MED17
 MED11 antibody  >2 publications with MED22 and MED11
 MED18 antibody  >1 publications with MED22 and MED18

Pathways associated with anti-MED22 antibody

 Products by Pathway  Pathway Diagram
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 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
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 PPARA Activates Gene Expression Pathway antibodies  PPARA Activates Gene Expression Pathway Diagram
 Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway antibodies  Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway Diagram
 Transcriptional Regulation Of White Adipocyte Differentiation Pathway antibodies  Transcriptional Regulation Of White Adipocyte Differentiation Pathway Diagram

Organs/Tissues associated with anti-MED22 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1 publications with MED22 and Muscle
 Heart Antibodies  >1 publications with MED22 and Heart
 Testis Antibodies  >1 publications with MED22 and Testis
 Brain Antibodies  >1 publications with MED22 and Brain
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