• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

MKK3/MKK6 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9613826

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13… More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MKK3/MKK6, Polyclonal Antibody
Full Product Name
MKK3/MKK6 Antibody
Product Gene Name
anti-MKK3/MKK6 antibody
Product Synonym Gene Name
MAP2K6, MAP2K3
Product Synonym Names
AW212142; dual specificity mitogen activated protein kinase kinase 3; Dual specificity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; map2k3; MAPK ERK kinase 3; MAPK/ERK kinase 3; MAPKK 3; MAPKK3; MEK 3; MEK3; Mitogen activated protein kinase kinase 3; MKK 3; MKK3; mMKK3b; MP2K3_HUMAN; PRKMK 3; PRKMK3; protein kinase, mitogen-activated, kinase 3; SAPK kinase 2; SAPKK 2; SAPKK2; Stress activated protein kinase kinase 2; Dual specificity mitogen activated protein kinase kinase 6; Dual specificity mitogen-activated protein kinase kinase 6; MAP kinase kinase 6; MAP2K6; MAPK/ERK kinase 6; MAPKK 6; MAPKK6; MEK 6; MEK6; Mitogen Activated Protein Kinase Kinase 6; MKK 6; MKK6; MP2K6_HUMAN; PRKMK6; Protein kinase mitogen activated kinase 6; protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6); SAPK kinase 3; SAPKK-3; SAPKK3; SKK3; Stress-activated protein kinase kinase 3
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Concentration
1mg/ml (lot specific)
Specificity
MKK3/MKK6 Antibody detects endogenous levels of total MKK3/MKK6.
Tissue Specificity: Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
MKK3/MKK6 peptide (MBS9617907) is used for blocking the activity of MKK3/MKK6 antibody (MBS9613826)
Molecular Weight
39,318 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (21187968..21218552). Location: 17q11.2
OMIM
602315
3D Structure
ModBase 3D Structure for P46734
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human MKK3/MKK6.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Weakly autophosphorylated. Phosphorylated at Ser-207 and Thr-211 by the majority of M3Ks, such as MAP3K5/ASK1, MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK3, MAP3K4/MEKK4, MAP3K7/TAK1, MAP3K11/MLK3 and MAP3K17/TAOK2. Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
Subunit Structure
Dimer. Interacts with Yersinia yopJ. Interacts (via its D domain) with its substrates MAPK11, MAPK12, MAPK13 and MAPK14 (By similarity). Interacts (via its DVD domain) with MAP3Ks activators like MAP3K5/ASK1, MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK3, MAP3K4/MEKK4, MAP3K7/TAK1, MAP3K11/MLK3 and MAP3K17/TAOK2 (By similarity). Interacts with DCTN1. Interacts with EIF2AK2/PKR.
Similarity
The DVD domain (residues 311-334) contains a conserved docking site and is found in the mammalian MAP kinase kinases (MAP2Ks). The DVD sites bind to their specific upstream MAP kinase kinase kinases (MAP3Ks) and are essential for activation.The D domain (residues 4-19) contains a conserved docking site and is required for the binding to MAPK substrates.Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Subcellular Location
Nucleus. Cytoplasm. Cytoplasm>Cytoskeleton.
Note: Binds to microtubules.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MKK3/MKK6 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Application Notes for anti-MKK3/MKK6 antibody
IF/ICC: 1:100-1:500
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000

Western Blot (WB) of anti-MKK3/MKK6 antibody

Western blot analysis of extracts from B16F10 cells(UV treatment), using MKK3/MKK6 Antibody. The lane on the left was treated with blocking peptide.
Rabbit Anti-MKK3/MKK6 Polyclonal Antibody – Cat# MBS9613826

Immunofluorescence (IF)/Immunocytochemistry (ICC) of anti-MKK3/MKK6 antibody

Staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AF6805) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.
The nuclear counter stain is DAPI (blue).
Rabbit Anti-MKK3/MKK6 Polyclonal Antibody, Immunofluorescence (IF)/Immunocytochemistry – Cat# MBS9613826

Other Notes

Small volumes of anti-MKK3/MKK6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MKK3/MKK6 purchase

MBS9613826 is an antibody targeting MKK3/MKK6. Also known as MKK3/MKK6 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MKK3/MKK6. It may not necessarily be applicable to this product.

NCBI GI #
21618347
NCBI GeneID
5606
NCBI Accession #
NP_002747.2
UniProt Primary Accession #
P46734
UniProt Secondary Accession #
P52564
UniProt Related Accession #
P46734
GenBank Nucleotide #
NM_002756.4
NCBI Official Full Name
dual specificity mitogen-activated protein kinase kinase 3 isoform A
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase 3
NCBI Official Symbol
MAP2K3
NCBI Official Synonym Symbols
MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
UniProt Protein Name
Dual specificity mitogen-activated protein kinase kinase 3
UniProt Synonym Protein Names
MAPK/ERK kinase 3; MEK 3; Stress-activated protein kinase kinase 2
UniProt Gene Name
MAP2K3
UniProt Synonym Gene Names
MEK3; MKK3; PRKMK3; SKK2; MAP kinase kinase 3; MAPKK 3; MEK 3; SAPK kinase 2; SAPKK-2; SAPKK2
UniProt Entry Name
MP2K3_HUMAN
NCBI Protein Information
dual specificity mitogen-activated protein kinase kinase 3; MEK 3; MAPKK 3; SAPK kinase 2; MAPK/ERK kinase 3; MAP kinase kinase 3; stress-activated protein kinase kinase 2

NCBI Summary for MKK3/MKK6

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for MKK3/MKK6

MKK3: a dual-specificity protein kinase of the STE7 family. Activates p38 MAP kinase by phosphorylating a Thr and a Tyr residue in the activation loop. Is activated by cytokines and environmental stress in vivo. A deletion and two point mutants found in colon cancer cell lines. Three splice-variant isoforms have been described.

Protein type: Protein kinase, dual-specificity (non-receptor); Kinase, protein; Protein kinase, STE; EC 2.7.12.2; STE group; STE7 family

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; membrane; cytosol

Molecular Function: MAP kinase kinase activity; protein binding; protein-tyrosine kinase activity; protein kinase binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; activation of MAPK activity; positive regulation of transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; pathogenesis; toll-like receptor 3 signaling pathway; signal transduction; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; positive regulation of protein kinase activity; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway; regulation of cytokine biosynthetic process; cardiac muscle contraction
Show more

Research Articles on MKK3/MKK6

1. miR-20a acts in a feedback loop to repress the expression of MKK3 and to negatively regulate the p38 pathway-mediated VEGF-induced endothelial cell migration and angiogenesis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MKK3/MKK6 antibody

 Reference Product  PubMed Publications
 MAPK14 antibody  >14 publications with MKK3/MKK6 and MAPK14
 MAPK3 antibody  >8 publications with MKK3/MKK6 and MAPK3
 MAP3K4 antibody  >3 publications with MKK3/MKK6 and MAP3K4
 DYRK1B antibody  >2 publications with MKK3/MKK6 and DYRK1B
 SMAD7 antibody  >1 publications with MKK3/MKK6 and SMAD7
 ELK1 antibody  >1 publications with MKK3/MKK6 and ELK1

Pathways associated with anti-MKK3/MKK6 antibody

 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 CDC42 Signaling Events Pathway antibodies  CDC42 Signaling Events Pathway Diagram
 CXCR3-mediated Signaling Events Pathway antibodies  CXCR3-mediated Signaling Events Pathway Diagram
 Cellular Roles Of Anthrax Toxin Pathway antibodies  Cellular Roles Of Anthrax Toxin Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram

Diseases associated with anti-MKK3/MKK6 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >37 publications with MKK3/MKK6 and Necrosis
 Neoplasms Antibodies  >30 publications with MKK3/MKK6 and Neoplasms
 Inflammation Antibodies  >22 publications with MKK3/MKK6 and Inflammation
 Heart Diseases Antibodies  >11 publications with MKK3/MKK6 and Heart Diseases
 Fibrosis Antibodies  >7 publications with MKK3/MKK6 and Fibrosis
 Nervous System Diseases Antibodies  >6 publications with MKK3/MKK6 and Nervous System Diseases
 Cardiomyopathies Antibodies  >6 publications with MKK3/MKK6 and Cardiomyopathies
 Cell Transformation, Neoplastic Antibodies  >5 publications with MKK3/MKK6 and Cell Transformation, Neoplastic
 Drug Toxicity Antibodies  >5 publications with MKK3/MKK6 and Drug Toxicity
 Breast Neoplasms Antibodies  >4 publications with MKK3/MKK6 and Breast Neoplasms

Organs/Tissues associated with anti-MKK3/MKK6 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >35 publications with MKK3/MKK6 and Blood
 Muscle Antibodies  >20 publications with MKK3/MKK6 and Muscle
 Vascular Antibodies  >15 publications with MKK3/MKK6 and Vascular
 Heart Antibodies  >13 publications with MKK3/MKK6 and Heart
 Kidney Antibodies  >11 publications with MKK3/MKK6 and Kidney
 Lung Antibodies  >11 publications with MKK3/MKK6 and Lung
 Bone Antibodies  >9 publications with MKK3/MKK6 and Bone
 Brain Antibodies  >9 publications with MKK3/MKK6 and Brain
 Skin Antibodies  >6 publications with MKK3/MKK6 and Skin
 Liver Antibodies  >5 publications with MKK3/MKK6 and Liver
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000