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MLKL Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758089

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
MLKL, Polyclonal Antibody
Full Product Name
Anti-MLKL Antibody Picoband
Product Gene Name
anti-MLKL antibody
Product Synonym Names
Serine/threonine-protein kinase AtPK1/AtPK6; 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Expressed in all tissues.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
54,479 Da
Chromosome Location
Chromosome: 16; NC_000016.9 (74705753..74734789, complement). Location: 16q23.1
3D Structure
ModBase 3D Structure for Q8NB16
Immunogen
E coli-derived human MLKL recombinant protein (Position: R210-K464). Human MLKL shares 69.7% amino acid (aa) sequence identity with mouse MLKL.
Subcellular Localization
Cytoplasm, Nucleus
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross reactivity with other proteins.
Protein Function
Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.

Product Categories/Family for anti-MLKL antibody

Primary Antibodies; Signal Transduction; Signaling Pathway; Small G Proteins
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-MLKL antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-MLKL antibody
WB: 0.25-0.5ug/ml, Human, Mouse, Rat
IHC: 2-5ug/ml, Human
ICC/IF: 5ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-MLKL antibody

Figure 1. Western blot analysis of MLKL using anti-MLKL antibody (MBS1758089).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLKL antigen affinity purified polyclonal antibody (#MBS1758089) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for MLKL at approximately 50 kDa. The expected band size for MLKL is at 54 kDa.
Rabbit Anti-MLKL Polyclonal Antibody – Cat# MBS1758089

Immunohistochemistry (IHC) of anti-MLKL antibody

Figure 2. IHC analysis of MLKL using anti-MLKL antibody (MBS1758089).
MLKL was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MLKL Antibody (MBS1758089) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-MLKL Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1758089

Immunohistochemistry (IHC) of anti-MLKL antibody

Figure 3. IHC analysis of MLKL using anti-MLKL antibody (MBS1758089).
MLKL was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MLKL Antibody (MBS1758089) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-MLKL Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1758089

Immunohistochemistry (IHC) of anti-MLKL antibody

Figure 4. IHC analysis of MLKL using anti-MLKL antibody (MBS1758089).
MLKL was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MLKL Antibody (MBS1758089) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-MLKL Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1758089

Immunohistochemistry (IHC) of anti-MLKL antibody

Figure 5. IHC analysis of MLKL using anti-MLKL antibody (MBS1758089).
MLKL was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MLKL Antibody (MBS1758089) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-MLKL Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1758089

Immunohistochemistry (IHC) of anti-MLKL antibody

Figure 6. IHC analysis of MLKL using anti-MLKL antibody (MBS1758089).
MLKL was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MLKL Antibody (MBS1758089) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-MLKL Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1758089

Immunohistochemistry (IHC) of anti-MLKL antibody

Figure 7. IHC analysis of MLKL using anti-MLKL antibody (MBS1758089).
MLKL was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MLKL Antibody (MBS1758089) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-MLKL Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1758089

Immunofluorescence (IF) of anti-MLKL antibody

Figure 8. IF analysis of MLKL using anti-MLKL antibody (MBS1758089).
MLKL was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (#MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-MLKL Antibody (MBS1758089) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Rabbit Anti-MLKL Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1758089

Flow Cytometry (FC/FACS) of anti-MLKL antibody

Figure 9. Flow Cytometry analysis of MCF-7 cells using anti-MLKL antibody (MBS1758089).
Overlay histogram showing MCF-7 cells stained with MBS1758089 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MLKL Antibody (MBS1758089, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-MLKL Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758089

Product References and Citations for anti-MLKL antibody

1. Dastani, Z., Pajukanta, P., Marcil, M., Rudzicz, N., Ruel, I., Bailey, S. D., Lee, J. C., Lemire, M., Faith, J., Platko, J., Rioux, J., Hudson, T. J., Gaudet, D., Engert, J. C., Genest, J. Fine mapping and association studies of a high-density lipoprotein cholesterol linkage region on chromosome 16 in French-Canadian subjects. Europ. J. Hum. Genet. 18: 342-347, 2010.2. Sun, L., Wang, H., Wang, Z., He, S., Chen, S., Liao, D., Wang, L., Yan, J., Liu, W., Lei, X., Wang, X. Mixed lineage kinase domain-like protein mediates necrosis signaling downstream of RIP3 kinase. Cell 148: 213-227, 2012.3. Wang, Z., Jiang, H., Chen, S., Du, F., Wang, X. The mitochondrial phosphatase PGAM5 functions at the convergence point of multiple necrotic death pathways. Cell 148: 228-243, 2012.

Other Notes

Small volumes of anti-MLKL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MLKL purchase

MBS1758089 is an antibody targeting MLKL. Also known as Anti-MLKL Antibody Picoband. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MLKL. It may not necessarily be applicable to this product.

NCBI GI #
215820622
NCBI GeneID
197259
NCBI Accession #
NP_001135969.1
UniProt Primary Accession #
Q8NB16
UniProt Secondary Accession #
Q8N6V0; A6NCE4
UniProt Related Accession #
Q8NB16
GenBank Nucleotide #
NM_001142497.1
NCBI Official Full Name
mixed lineage kinase domain-like protein isoform 2
NCBI Official Synonym Full Names
mixed lineage kinase domain-like
NCBI Official Symbol
MLKL
UniProt Protein Name
Mixed lineage kinase domain-like protein
Protein Family
Mixed lineage kinase domain-like protein
UniProt Gene Name
MLKL
UniProt Entry Name
MLKL_HUMAN
NCBI Protein Information
mixed lineage kinase domain-like protein

NCBI Summary for MLKL

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]
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UniProt Comments for MLKL

MLKL: Belongs to the protein kinase superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; TKL-Unique family

Chromosomal Location of Human Ortholog: 16q23.1

Cellular Component: cytoplasm; plasma membrane

Molecular Function: protein binding; protein complex binding; protein kinase binding; ATP binding; protein kinase activity

Biological Process: protein amino acid phosphorylation
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Research Articles on MLKL

1. study suggests that MLKL is a key RIP3 downstream component of TNF-induced necrotic cell death

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MLKL antibody

 Products by Pathway  Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 TNF Signaling Pathway antibodies  TNF Signaling Pathway Diagram
 TNF Signaling Pathway antibodies  TNF Signaling Pathway Diagram

Diseases associated with anti-MLKL antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >9 publications with MLKL and Necrosis
 Inflammation Antibodies  >3 publications with MLKL and Inflammation
 Neoplasms Antibodies  >1 publications with MLKL and Neoplasms
 Immune System Diseases Antibodies  >1 publications with MLKL and Immune System Diseases

Organs/Tissues associated with anti-MLKL antibody

 Organ/Tissue Name  Pubmed Publications
 Uncharacterized Tissue Antibodies  >1 publications with MLKL and Uncharacterized Tissue
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