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MYL12B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS129096
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The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001) More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MYL12B, Polyclonal Antibody
Full Product Name
MYL12B Polyclonal Antibody
Product Gene Name
Product Synonym Names
MLC-B; MRLC2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Molecular Weight
172
3D Structure
ModBase 3D Structure for O14950
Species
Human
Immunogen
Recombinant protein of human MYL12B
Calculated Molecular Weight
20kDa

Product Categories/Family for anti-MYL12B antibody

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MYL12B antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-MYL12B antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Western Blot (WB) of anti-MYL12B antibody

Western blot analysis of extracts of various cell lines, using MYL12B antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Rabbit Anti-MYL12B Polyclonal Antibody – Cat# MBS129096

Other Notes

Small volumes of anti-MYL12B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MYL12B purchase

MBS129096 is an antibody targeting MYL12B. Also known as MYL12B Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MYL12B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Myosin regulatory light chain 12B
NCBI Official Synonym Full Names
myosin, light chain 12B, regulatory
NCBI Official Symbol
MYL12B
NCBI Official Synonym Symbols
MLC-B; MRLC2
UniProt Protein Name
Myosin regulatory light chain 12B
UniProt Synonym Protein Names
MLC-2A; MLC-2; Myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa; MLC20; Myosin regulatory light chain MRLC2; SHUJUN-1
Protein Family
UniProt Gene Name
MYL12B
UniProt Synonym Gene Names
MRLC2; MYLC2B; MLC-2; MLC20
UniProt Entry Name
ML12B_HUMAN
NCBI Protein Information
myosin regulatory light chain 12B; MLC-2; MLC20; MLC-2A; SHUJUN-1; myosin regulatory light chain 2; myosin regulatory light chain MRLC2; myosin regulatory light chain 20 kDa; myosin regulatory light chain 2-B, smooth muscle isoform

NCBI Summary for MYL12B

The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).[supplied by OMIM, Mar 2008]
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UniProt Comments for MYL12B

MRLC2: non-sarcomeric myosin regulatory light chain 2. Binds calcium and plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity. Phosphorylation of MLC-2 in the presence of calcium and calmodulin increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. Myosin is an hexamer of 2 heavy chains and 4 light chains.

Protein type: Contractile

Chromosomal Location of Human Ortholog: 18p11.31

Cellular Component: myosin II complex; apical part of cell; stress fiber; Z disc; cytosol

Molecular Function: protein binding; calcium ion binding

Biological Process: axon guidance; regulation of cell shape; muscle contraction; ephrin receptor signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MYL12B antibody

Organs/Tissues associated with anti-MYL12B antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >2 publications with MYL12B and Muscle
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