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NFIA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758405

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NFIA, Polyclonal Antibody
Full Product Name
Anti-NFIA Antibody Picoband
Product Gene Name
anti-NFIA antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
509
OMIM
600727
3D Structure
ModBase 3D Structure for Q12857
Immunogen
E coli-derived human NFIA recombinant protein (Position: V172-Q233). Human NFIA shares 100% amino acid (aa) sequence identity with rat NFIA.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.

Product Categories/Family for anti-NFIA antibody

Primary Antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-NFIA antibody
Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-NFIA antibody
WB: 0.25-0.5ug/ml, Human, Mouse, Rat
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-NFIA antibody

Figure 1. Western blot analysis of NFIA using anti-NFIA antibody (MBS1758405).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: mouse L929 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFIA antigen affinity purified polyclonal antibody (#MBS1758405) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for NFIA at approximately 70 kDa. The expected band size for NFIA is at 56 kDa.
Rabbit Anti-NFIA Polyclonal Antibody – Cat# MBS1758405

Flow Cytometry (FC/FACS) of anti-NFIA antibody

Figure 2. Flow Cytometry analysis of JK cells using anti-NFIA antibody (MBS1758405).
Overlay histogram showing JK cells stained with MBS1758405 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFIA Antibody (MBS1758405, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Rabbit Anti-NFIA Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758405

Product References and Citations for anti-NFIA antibody

1. Campbell, C. G. N., Wang, H., Hunter, G. W. Interstitial microdeletion of chromosome 1p in two siblings. Am. J. Med. Genet. 111: 289-294, 2002.2. das Neves, L., Duchala, C. S., Godinho, F., Haxhiu, M. A., Colmenares, C., Macklin, W. B., Campbell, C. E., Butz, K. G., Gronostajski, R. M. Disruption of the murine nuclear factor I-A gene (Nfia) results in perinatal lethality, hydrocephalus, and agenesis of the corpus callosum. Proc. Nat. Acad. Sci. 96: 11946-11951, 1999. Note: Erratum: Proc. Nat. Acad. Sci. 98: 4276 only, 2001. 3. Deneen, B., Ho, R., Lukaszewicz, A., Hochstim, C. J., Gronostajski, R. M., Anderson, D. J. The transcription factor NFIA controls the onset of gliogenesis in the developing spinal cord. Neuron 52: 953-968, 2006.

Other Notes

Small volumes of anti-NFIA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NFIA purchase

MBS1758405 is an antibody targeting NFIA. Also known as Anti-NFIA Antibody Picoband. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NFIA. It may not necessarily be applicable to this product.

NCBI GI #
14194959
NCBI GeneID
4774
NCBI Accession #
Q12857.2
UniProt Primary Accession #
Q12857
UniProt Secondary Accession #
Q8TA97; Q9H3X9; Q9P2A9; B4DRJ3; B4DS53; F5H0R0; F8W8W3
UniProt Related Accession #
Q12857
NCBI Official Full Name
Nuclear factor 1 A-type
NCBI Official Synonym Full Names
nuclear factor I/A
NCBI Official Symbol
NFIA
NCBI Official Synonym Symbols
CTF; NF1-A; NFI-A; NFI-L; NF-I/A
UniProt Protein Name
Nuclear factor 1 A-type
UniProt Synonym Protein Names
CCAAT-box-binding transcription factor; CTF; Nuclear factor I/A; NF-I/A; NFI-A; TGGCA-binding protein
Protein Family
Nuclear factor
UniProt Gene Name
NFIA
UniProt Synonym Gene Names
KIAA1439; NF1-A; Nuclear factor 1/A; CTF; NF-I/A; NFI-A
UniProt Entry Name
NFIA_HUMAN
NCBI Protein Information
nuclear factor 1 A-type; TGGCA-binding protein; CCAAT-box-binding transcription factor

NCBI Summary for NFIA

This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

UniProt Comments for NFIA

NFI-A: Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Belongs to the CTF/NF-I family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1p31.3-p31.2

Cellular Component: nucleoplasm; nucleus; cell junction

Molecular Function: transcription factor binding; transcription factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; viral genome replication; DNA replication; response to organic cyclic substance
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Research Articles on NFIA

1. High nuclear factor IA expression is associated with glioblastomas.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NFIA antibody

 Products by Pathway  Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway antibodies  RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway Diagram
 RNA Polymerase III Abortive And Retractive Initiation Pathway antibodies  RNA Polymerase III Abortive And Retractive Initiation Pathway Diagram
 RNA Polymerase III Transcription Pathway antibodies  RNA Polymerase III Transcription Pathway Diagram
 RNA Polymerase III Transcription Termination Pathway antibodies  RNA Polymerase III Transcription Termination Pathway Diagram

Diseases associated with anti-NFIA antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >17 publications with NFIA and Nervous System Diseases
 Congenital Abnormalities Antibodies  >7 publications with NFIA and Congenital Abnormalities
 Urogenital Abnormalities Antibodies  >2 publications with NFIA and Urogenital Abnormalities
 Inflammation Antibodies  >2 publications with NFIA and Inflammation
 Disorders of Sex Development Antibodies  >2 publications with NFIA and Disorders of Sex Development
 Kidney Diseases Antibodies  >2 publications with NFIA and Kidney Diseases
 Craniofacial Abnormalities Antibodies  >1 publications with NFIA and Craniofacial Abnormalities
 Disease Models, Animal Antibodies  >1 publications with NFIA and Disease Models, Animal
 Learning Disorders Antibodies  >1 publications with NFIA and Learning Disorders
 Hypertrophy Antibodies  >1 publications with NFIA and Hypertrophy

Organs/Tissues associated with anti-NFIA antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >40 publications with NFIA and Brain
 Embryonic Tissue Antibodies  >12 publications with NFIA and Embryonic Tissue
 Lung Antibodies  >8 publications with NFIA and Lung
 Thyroid Antibodies  >7 publications with NFIA and Thyroid
 Bone Antibodies  >7 publications with NFIA and Bone
 Liver Antibodies  >6 publications with NFIA and Liver
 Kidney Antibodies  >5 publications with NFIA and Kidney
 Eye Antibodies  >4 publications with NFIA and Eye
 Muscle Antibodies  >3 publications with NFIA and Muscle
 Heart Antibodies  >3 publications with NFIA and Heart
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