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NR0B1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9607241

Description: This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by… More details

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Product Overview

Online Datasheet in PDF
Product Name
NR0B1, Polyclonal Antibody
Full Product Name
NR0B1 Antibody
Product Gene Name
anti-NR0B1 antibody
Product Synonym Names
AHC; AHCH; AHX; DAX 1; DAX1; Dosage sensitive sex reversal; DSS; DSS AHC critical region on the X chromosome protein 1; DSS-AHC critical region on the X chromosome protein 1; GTD; HHG; Nr0b1; NR0B1_HUMAN; NROB1; Nuclear hormone receptor; Nuclear receptor 0B1; Nuclear receptor DAX 1; Nuclear receptor DAX-1; Nuclear receptor DAX1; Nuclear receptor subfamily 0 group B member 1; SRXY2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
peptide affinity purification
Concentration
1mg/ml (lot specific)
Specificity
NR0B1 antibody detects endogenous levels of total NR0B1
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
NR0B1 peptide (MBS9625957) is used for blocking the activity of NR0B1 antibody (MBS9607241)
3D Structure
ModBase 3D Structure for P51843
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human NR0B1, corresponding to a region within the internal amino acids.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-NR0B1 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-NR0B1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
ELISA(peptide): 1:20000-1:40000

Western Blot (WB) of anti-NR0B1 antibody

Western blot analysis of extracts of NR0B1protein, using NR0B1 antibody. The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-NR0B1 Polyclonal Antibody – Cat# MBS9607241

Immunohistochemistry (IHC) of anti-NR0B1 antibody

MBS9607241 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-NR0B1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9607241

Immunofluorescence (IF) of anti-NR0B1 antibody

MBS9607241 staining HepG2  cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Rabbit Anti-NR0B1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9607241

Western Blot (WB) of anti-NR0B1 antibody

Western blot analysis of extracts from rat liver, using NR0B1 Antibody.
Rabbit Anti-NR0B1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9607241

Immunohistochemistry (IHC) of anti-NR0B1 antibody

Immunohistochemistry (IHC) MBS9607241 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-NR0B1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9607241

Immunofluorescence (IF) of anti-NR0B1 antibody

Immunofluorescence (IF) staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9607241) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.
Rabbit Anti-NR0B1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9607241

Other Notes

Small volumes of anti-NR0B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NR0B1 purchase

MBS9607241 is an antibody targeting NR0B1. Also known as NR0B1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NR0B1. It may not necessarily be applicable to this product.

NCBI GI #
5016090
NCBI GeneID
190
NCBI Accession #
NP_000466.2
UniProt Primary Accession #
P51843
UniProt Secondary Accession #
Q96F69
UniProt Related Accession #
P51843
GenBank Nucleotide #
NM_000475.4
NCBI Official Full Name
nuclear receptor subfamily 0 group B member 1
NCBI Official Synonym Full Names
nuclear receptor subfamily 0 group B member 1
NCBI Official Symbol
NR0B1
NCBI Official Synonym Symbols
AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2
UniProt Protein Name
Nuclear receptor subfamily 0 group B member 1
UniProt Synonym Protein Names
DSS-AHC critical region on the X chromosome protein 1; Nuclear receptor DAX-1
Protein Family
Nuclear receptor subfamily
UniProt Gene Name
NR0B1
UniProt Synonym Gene Names
AHC; DAX1
NCBI Protein Information
nuclear receptor subfamily 0 group B member 1

NCBI Summary for NR0B1

This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism. [provided by RefSeq, Jul 2008]
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UniProt Comments for NR0B1

Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency ().
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Research Articles on NR0B1

1. A novel missense mutation (c.775T>C; p.ser259Pro) in the NROBI gene cause a late-onset adrenal insufficiency without hypogonadism.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NR0B1 antibody

 Reference Product  PubMed Publications
 NR5A1 antibody  >69 publications with NR0B1 and NR5A1
 PPARG antibody  >2 publications with NR0B1 and PPARG
 ESR1 antibody  >2 publications with NR0B1 and ESR1
 COPS2 antibody  >2 publications with NR0B1 and COPS2
 SREBF1 antibody  >1 publications with NR0B1 and SREBF1

Pathways associated with anti-NR0B1 antibody

 Products by Pathway  Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Nuclear Receptor Transcription Pathway antibodies  Nuclear Receptor Transcription Pathway Diagram
 Nuclear Receptors Pathway antibodies  Nuclear Receptors Pathway Diagram
 Regulation Of Androgen Receptor Activity Pathway antibodies  Regulation Of Androgen Receptor Activity Pathway Diagram

Diseases associated with anti-NR0B1 antibody

 Disease Name  Pubmed Publications
 Gonadal Disorders Antibodies  >129 publications with NR0B1 and Gonadal Disorders
 Congenital Abnormalities Antibodies  >83 publications with NR0B1 and Congenital Abnormalities
 Dosage-sensitive sex reversal Antibodies  >75 publications with NR0B1 and Dosage-sensitive sex reversal
 Neoplasms Antibodies  >52 publications with NR0B1 and Neoplasms
 Adrenal Hyperplasia, Congenital Antibodies  >27 publications with NR0B1 and Adrenal Hyperplasia, Congenital
 Carcinoma Antibodies  >16 publications with NR0B1 and Carcinoma
 Wilms Tumor Antibodies  >16 publications with NR0B1 and Wilms Tumor
 Infertility, Male Antibodies  >15 publications with NR0B1 and Infertility, Male
 Infertility, Female Antibodies  >14 publications with NR0B1 and Infertility, Female
 Ovarian Neoplasms Antibodies  >8 publications with NR0B1 and Ovarian Neoplasms

Organs/Tissues associated with anti-NR0B1 antibody

 Organ/Tissue Name  Pubmed Publications
 Adrenal Gland Antibodies  >112 publications with NR0B1 and Adrenal Gland
 Testis Antibodies  >102 publications with NR0B1 and Testis
 Brain Antibodies  >23 publications with NR0B1 and Brain
 Mixed Antibodies  >4 publications with NR0B1 and Mixed
 Lung Antibodies  >4 publications with NR0B1 and Lung
 Pancreas Antibodies  >3 publications with NR0B1 and Pancreas
 Bladder Antibodies  >1 publications with NR0B1 and Bladder
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