• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

NUMBL Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758873

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NUMBL, Polyclonal Antibody
Full Product Name
Anti-NUMBL Antibody Picoband
Product Gene Name
anti-NUMBL antibody
Product Synonym Names
Serine/threonine-protein kinase AtPK1/AtPK6; 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Expressed in all tissues.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
609
OMIM
604018
3D Structure
ModBase 3D Structure for Q9Y6R0
Immunogen
E coli-derived human NUMBL recombinant protein (Position: R336-A537). Human NUMBL shares 91.1% and 89.4% amino acid (aa) sequence identity with mouse and rat NUMBL, respectively.
Subcellular Localization
Cytoplasm, Nucleus
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross reactivity with other proteins.
Protein Function
Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.

Product Categories/Family for anti-NUMBL antibody

Primary Antibodies; Signal Transduction; Signaling Pathway; Small G Proteins
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-NUMBL antibody
Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-NUMBL antibody
WB: 0.25-0.5ug/ml, Human, Mouse, Rat
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-NUMBL antibody

Figure 1. Western blot analysis of NUMBL using anti-NUMBL antibody (MBS1758873).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U-87 MG whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUMBL antigen affinity purified polyclonal antibody (#MBS1758873) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for NUMBL at approximately 72 kDa. The expected band size for NUMBL is at 65-70 kDa.
Rabbit Anti-NUMBL Polyclonal Antibody – Cat# MBS1758873

Flow Cytometry (FC/FACS) of anti-NUMBL antibody

Figure 2. Flow Cytometry analysis of U87 cells using anti-NUMBL antibody (MBS1758873).
Overlay histogram showing U87 cells stained with MBS1758873 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUMBL Antibody (MBS1758873, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-NUMBL Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758873

Product References and Citations for anti-NUMBL antibody

1. Huang, E. J., Li, H., Tang, A. A., Wiggins, A. K., Neve, R. L., Zhong, W., Jan, L. Y., Jan, Y. N. Targeted deletion of numb and numblike in sensory neurons reveals their essential functions in axon arborization. Genes Dev. 19: 138-151, 2005. 2. Li, H.-S., Wang, D., Shen, Q., Schonemann, M. D., Gorski, J. A., Jones, K. R., Temple, S., Jan, L. Y., Jan, Y. N. Inactivation of Numb and Numblike in embryonic dorsal forebrain impairs neurogenesis and disrupts cortical morphogenesis. Neuron 40: 1105-1118, 2003. 3. Margolis, R. L., Abraham, M. R., Gatchell, S. B., Li, S.-H., Kidwai, A. S., Breschel, T. S., Stine, O. C., Callahan, C., McInnis, M. G., Ross, C. A. cDNAs with long CAG trinucleotide repeats from human brain. Hum. Genet. 100: 114-122, 1997.

Other Notes

Small volumes of anti-NUMBL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NUMBL purchase

MBS1758873 is an antibody targeting NUMBL. Also known as Anti-NUMBL Antibody Picoband. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NUMBL. It may not necessarily be applicable to this product.

NCBI GI #
14194976
NCBI GeneID
9253
NCBI Accession #
Q9Y6R0.1
UniProt Primary Accession #
Q9Y6R0
UniProt Secondary Accession #
Q7Z4J9
UniProt Related Accession #
Q9Y6R0
NCBI Official Full Name
Numb-like protein
NCBI Official Synonym Full Names
numb homolog (Drosophila)-like
NCBI Official Symbol
NUMBL
NCBI Official Synonym Symbols
NBL; CAG3A; CTG3a; NUMBR; NUMB-R; TNRC23; NUMBLIKE
UniProt Protein Name
Numb-like protein
UniProt Synonym Protein Names
Numb-related protein; Numb-R
Protein Family
Numb-like protein
UniProt Gene Name
NUMBL
UniProt Synonym Gene Names
Numb-R
UniProt Entry Name
NUMBL_HUMAN
NCBI Protein Information
numb-like protein; numb homolog-like; numb-related protein

UniProt Comments for NUMBL

NUMBL: Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF- kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons. Interacts (via PTB domain) with MAP3K7IP2 (via C- terminal). Interacts (via C-terminal) with TRAF6 (via TRAF domains). Associates with EPS15 and NOTCH1.

Protein type: Cell development/differentiation

Chromosomal Location of Human Ortholog: 19q13.13-q13.2

Cellular Component: cytoplasm

Molecular Function: protein binding

Biological Process: nervous system development; positive regulation of neurogenesis; axonogenesis; cytokine and chemokine mediated signaling pathway; protein metabolic process; neuroblast division in the subventricular zone; positive regulation of dendrite morphogenesis; lateral ventricle development
Show more

Research Articles on NUMBL

1. Numbl might be involved in the inhibition of growth, proliferation, and invasion of 95-D lung cancer cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NUMBL antibody

 Reference Product  PubMed Publications
 NOTCH1 antibody  >7 publications with NUMBL and NOTCH1
 TRAF6 antibody  >3 publications with NUMBL and TRAF6
 APP antibody  >2 publications with NUMBL and APP
 NOTCH3 antibody  >2 publications with NUMBL and NOTCH3
 TAB2 antibody  >2 publications with NUMBL and TAB2

Pathways associated with anti-NUMBL antibody

 Products by Pathway  Pathway Diagram
 Delta-Notch Signaling Pathway antibodies  Delta-Notch Signaling Pathway Diagram
 Notch Signaling Pathway antibodies  Notch Signaling Pathway Diagram
 Notch Signaling Pathway antibodies  Notch Signaling Pathway Diagram
 Notch Signaling Pathway antibodies  Notch Signaling Pathway Diagram

Diseases associated with anti-NUMBL antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >4 publications with NUMBL and Nervous System Diseases
 Necrosis Antibodies  >4 publications with NUMBL and Necrosis

Organs/Tissues associated with anti-NUMBL antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >33 publications with NUMBL and Nerve
 Brain Antibodies  >19 publications with NUMBL and Brain
 Embryonic Tissue Antibodies  >16 publications with NUMBL and Embryonic Tissue
 Heart Antibodies  >4 publications with NUMBL and Heart
 Eye Antibodies  >3 publications with NUMBL and Eye
 Lung Antibodies  >3 publications with NUMBL and Lung
 Muscle Antibodies  >3 publications with NUMBL and Muscle
 Thymus Antibodies  >3 publications with NUMBL and Thymus
 Testis Antibodies  >2 publications with NUMBL and Testis
 Prostate Antibodies  >1 publications with NUMBL and Prostate
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000