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p27 KIP1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9504228

MBS9504228 is an antibody targeting p27 Kip1. Also known as p27 Kip1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$300 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
p27 Kip1, Polyclonal Antibody
Full Product Name
p27 Kip1 Antibody
Product Gene Name
Product Synonym Gene Name
CDKN1B
Product Synonym Names
CDKN1B; CDN1B; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; KIP1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Concentration
1 mg/ml (lot specific)
Specificity
p27 Kip1 Antibody detects endogenous levels of total p27 Kip1 protein.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Molecular Weight
22 kDa
OMIM
U10906 mRNA
3D Structure
ModBase 3D Structure for P46527
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human p27 Kip1.
Immunogen Range
Jan-50
Applications Tested/Suitable for anti-p27 Kip1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-p27 Kip1 antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:10000

Immunohistochemistry (IHC) of anti-p27 Kip1 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using p27 Kip1 Antibody. The picture on the right is blocked with the synthesized peptide.
Rabbit Anti-p27 KIP1 Polyclonal Antibody – Cat# MBS9504228

Western Blot (WB) of anti-p27 Kip1 antibody

Western blot analysis of lysates from A2780 cells, using p27 Kip1 Antibody. The lane on the right is blocked with the synthesized peptide.
Rabbit Anti-p27 KIP1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9504228

Other Notes

Small volumes of anti-p27 Kip1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for p27 Kip1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cyclin-dependent kinase inhibitor 1B
NCBI Official Synonym Full Names
cyclin dependent kinase inhibitor 1B
NCBI Official Symbol
CDKN1B
NCBI Official Synonym Symbols
KIP1; MEN4; CDKN4; MEN1B; P27KIP1
UniProt Protein Name
Cyclin-dependent kinase inhibitor 1B
UniProt Synonym Protein Names
Cyclin-dependent kinase inhibitor p27
UniProt Gene Name
CDKN1B
UniProt Synonym Gene Names
KIP1
NCBI Protein Information
cyclin-dependent kinase inhibitor 1B

NCBI Summary for p27 Kip1

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]
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UniProt Comments for p27 Kip1

Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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