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PAN2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758434

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PAN2, Polyclonal Antibody
Full Product Name
Anti-PAN2 Antibody Picoband
Product Gene Name
anti-PAN2 antibody
Product Synonym Names
AF4/FMR2 family member 4; AF4/FMR2 family member 4; ALL1-fused gene from chromosome 5q31 protein; Protein AF-5q31; Major CDK9 elongation factor-associated protein; AFF4; AF5Q31, MCEF; HSPC092
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Ubiquitously expressed. StronglyExpressed in heart, placenta, skeletal muscle, pancreas and to a lower extent in brain.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
135,281 Da
3D Structure
ModBase 3D Structure for Q504Q3
Immunogen
E coli-derived human PAN2 recombinant protein (Position: M1-L356).
Subcellular Localization
Nucleus, Associates to transcriptionally active chromatin but not at snRNA genes.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression.

Product Categories/Family for anti-PAN2 antibody

Primary Antibodies; Epigenetics and Nuclear Signaling; Transcription; Cell Biology; Transcription Factors; Cell Cycle
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-PAN2 antibody
Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-PAN2 antibody
WB: 0.1-0.25ug/ml, Human, Mouse, Rat
FC/FACS (Fixed): 1-3ug/1x1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-PAN2 antibody

Figure 1. Western blot analysis of PAN2 using anti-PAN2 antibody (MBS1758434).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hel whole cell lysates,
Lane 3: human Daudi whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAN2 antigen affinity purified polyclonal antibody (#MBS1758434) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for PAN2 at approximately 135 kDa. The expected band size for PAN2 is at 135 kDa.
Rabbit Anti-PAN2 Polyclonal Antibody – Cat# MBS1758434

Flow Cytometry (FC/FACS) of anti-PAN2 antibody

Figure 2. Flow Cytometry analysis of PC-3 cells using anti-PAN2 antibody (MBS1758434).
Overlay histogram showing PC-3 cells stained with MBS1758434 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAN2 Antibody (MBS1758434, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-PAN2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758434

Product References and Citations for anti-PAN2 antibody

1. Bett, J. S., Ibrahim, A. F. M., Garg, A. K., Kelly, V., Pedrioli, P., Rocha, S., Hay, R. T. The P-body component USP52/PAN2 is a novel regulator of HIF1A mRNA stability. Biochem. J. 451: 185-194, 2013. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 04/21/2017.3. Ishikawa, K., Nagase, T., Suyama, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 5: 169-176, 1998.

Other Notes

Small volumes of anti-PAN2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAN2 purchase

MBS1758434 is an antibody targeting PAN2. Also known as Anti-PAN2 Antibody Picoband. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PAN2. It may not necessarily be applicable to this product.

NCBI GI #
261878449
NCBI GeneID
9924
NCBI Accession #
NP_001159751
UniProt Primary Accession #
Q504Q3
UniProt Secondary Accession #
O75189; Q76E12; Q8IVE1
UniProt Related Accession #
Q504Q3
GenBank Nucleotide #
NM_001127460
NCBI Official Full Name
PAB-dependent poly(A)-specific ribonuclease subunit PAN2 isoform 2
NCBI Official Synonym Full Names
PAN2 poly(A) specific ribonuclease subunit
NCBI Official Symbol
PAN2
NCBI Official Synonym Symbols
USP52
UniProt Protein Name
PAB-dependent poly(A)-specific ribonuclease subunit PAN2
UniProt Synonym Protein Names
Inactive ubiquitin carboxyl-terminal hydrolase 52; PAB1P-dependent poly(A)-nuclease; PAN deadenylation complex catalytic subunit 2
Protein Family
PAB-dependent poly(A)-specific ribonuclease
UniProt Gene Name
PAN2
UniProt Synonym Gene Names
hPan2
UniProt Entry Name
PAN2_HUMAN
NCBI Protein Information
PAB-dependent poly(A)-specific ribonuclease subunit PAN2; PAB-dependent poly(A)-specific ribonuclease subunit PAN2; PAB-dependent poly(A)-specific ribonuclease subunit 2; PAB1P-dependent poly(A)-nuclease; PABP-dependent poly(A) nuclease 2; PAN deadenylati

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PAN2 antibody

 Reference Product  PubMed Publications
 PAN3 antibody  >24 publications with PAN2 and PAN3
 PABPC1 antibody  >5 publications with PAN2 and PABPC1

Pathways associated with anti-PAN2 antibody

 Products by Pathway  Pathway Diagram
 Deadenylation Of MRNA Pathway antibodies  Deadenylation Of MRNA Pathway Diagram
 Deadenylation-dependent MRNA Decay Pathway antibodies  Deadenylation-dependent MRNA Decay Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 RNA Degradation Pathway antibodies  RNA Degradation Pathway Diagram
 RNA Degradation Pathway antibodies  RNA Degradation Pathway Diagram

Diseases associated with anti-PAN2 antibody

 Disease Name  Pubmed Publications
 Death Antibodies  >4 publications with PAN2 and Death
 Carcinoma Antibodies  >2 publications with PAN2 and Carcinoma
 Thrombosis Antibodies  >1 publications with PAN2 and Thrombosis
 Cardiovascular Diseases Antibodies  >1 publications with PAN2 and Cardiovascular Diseases
 Inflammation Antibodies  >1 publications with PAN2 and Inflammation
 Necrosis Antibodies  >1 publications with PAN2 and Necrosis

Organs/Tissues associated with anti-PAN2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >9 publications with PAN2 and Blood
 Pancreas Antibodies  >3 publications with PAN2 and Pancreas
 Kidney Antibodies  >2 publications with PAN2 and Kidney
 Lung Antibodies  >2 publications with PAN2 and Lung
 Muscle Antibodies  >2 publications with PAN2 and Muscle
 Embryonic Tissue Antibodies  >2 publications with PAN2 and Embryonic Tissue
 Heart Antibodies  >2 publications with PAN2 and Heart
 Adipose Tissue Antibodies  >1 publications with PAN2 and Adipose Tissue
 Liver Antibodies  >1 publications with PAN2 and Liver
 Nerve Antibodies  >1 publications with PAN2 and Nerve
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