• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PAR4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9606664

Function: Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation. More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PAR4, Polyclonal Antibody
Full Product Name
PAR4 Antibody
Product Gene Name
anti-PAR4 antibody
Product Synonym Gene Name
F2RL3
Product Synonym Names
Coagulation factor II (thrombin) receptor like 3; Coagulation factor II receptor like 3; Coagulation factor II receptor-like 3; F2RL3; PAR 4; PAR-4; PAR4; PAR4_HUMAN; protease activated receptor 4; protease activated receptor4; Proteinase activated receptor 4; Proteinase-activated receptor 4; Thrombin receptor like 3; Thrombin receptor-like 3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific)
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
PAR4 peptide (MBS9625381) is used for blocking the activity of PAR4 antibody (MBS9606664)
Molecular Weight
41 KD
3D Structure
ModBase 3D Structure for Q96RI0
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human PAR4, corresponding to a region within N-terminal amino acids.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-PAR4 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-PAR4 antibody
WB: 1:500 - 1:1000, IF/ICC: 1:100 - 1:500

The optimal dilutions should be determined by the end user

IMPORTANT:
For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween20 at 4°C with gentle shaking, overnight.

Immunofluorescence (IF) of anti-PAR4 antibody

MBS9606664 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Rabbit Anti-PAR4 Polyclonal Antibody – Cat# MBS9606664

Western Blot (WB) of anti-PAR4 antibody

Western blot analysis of extracts from HeLa cells using PAR4 antibody.
Rabbit Anti-PAR4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9606664

Other Notes

Small volumes of anti-PAR4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAR4 purchase

MBS9606664 is an antibody targeting PAR4. Also known as PAR4 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PAR4. It may not necessarily be applicable to this product.

NCBI GI #
89353783
NCBI GeneID
9002
NCBI Accession #
NP_003941.2
UniProt Primary Accession #
Q96RI0
UniProt Secondary Accession #
O76067; Q6DK42
UniProt Related Accession #
Q96RI0
GenBank Nucleotide #
NM_003950.3
NCBI Official Full Name
proteinase-activated receptor 4 preproprotein
NCBI Official Synonym Full Names
F2R like thrombin or trypsin receptor 3
NCBI Official Symbol
F2RL3
NCBI Official Synonym Symbols
PAR4
UniProt Protein Name
Proteinase-activated receptor 4
UniProt Synonym Protein Names
Coagulation factor II receptor-like 3; Thrombin receptor-like 3
UniProt Gene Name
F2RL3
UniProt Synonym Gene Names
PAR4; PAR-4
NCBI Protein Information
proteinase-activated receptor 4

NCBI Summary for PAR4

This gene encodes a member of the protease-activated receptor subfamily, part of the G-protein coupled receptor 1 family of proteins. The encoded receptor is proteolytically processed to reveal an extracellular N-terminal tethered ligand that binds to and activates the receptor. This receptor plays a role in blood coagulation, inflammation and response to pain. Hypomethylation at this gene may be associated with lung cancer in human patients. [provided by RefSeq, Sep 2016]
Show more

UniProt Comments for PAR4

Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation.

Research Articles on PAR4

1. Data suggested that PAR4-AP-induced platelet reactivity between PAR4 rs773902 was associated with altered intensity of Ca(2+) mobilization and ERK activation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PAR4 antibody

 Reference Product  PubMed Publications
 F2RL2 antibody  >1 publications with PAR4 and F2RL2

Pathways associated with anti-PAR4 antibody

 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram

Organs/Tissues associated with anti-PAR4 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >1 publications with PAR4 and Lung
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000