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POLR2J Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9607309

Description: This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly… More details

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Product Overview

Online Datasheet in PDF
Product Name
POLR2J, Polyclonal Antibody
Full Product Name
POLR2J Antibody
Product Gene Name
anti-POLR2J antibody
Product Synonym Names
DNA directed RNA polymerase II subunit RPB11 a; DNA-directed RNA polymerase II 13.3 kDa polypeptide; DNA-directed RNA polymerase II 13.3 kDa polypeptide; DNA-directed RNA polymerase II subunit J 1; DNA-directed RNA polymerase II subunit J-1; DNA-directed RNA polymerase II subunit RPB11-a; hRPB 14; hRPB14; POLR 2J1; POLR2J; POLR2J1; polymerase (RNA) II (DNA directed) polypeptide J 13.3kDa; polymerase (RNA) II (DNA directed) polypeptide J 13.3kDa; RNA polymerase II 13.3 kDa subunit; RNA polymerase II subunit B11 a; RNA polymerase II subunit B11-a; RPB 11; RPB 11A; RPB 11m; RPB11; RPB11_HUMAN; RPB11a; RPB11m; Rpo2-4
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
POLR2J antibody detects endogenous levels of total POLR2J
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
RPB11 peptide (MBS9626025) is used for blocking the activity of RPB11 antibody (MBS9607309)
Molecular Weight
13 kDa
3D Structure
ModBase 3D Structure for P52435
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human POLR2J corresponding to a region within N-terminal amino acids.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-POLR2J antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-POLR2J antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-POLR2J antibody

MBS9607309 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Rabbit Anti-POLR2J Polyclonal Antibody – Cat# MBS9607309

Western Blot (WB) of anti-POLR2J antibody

Western blot analysis of K562 whole cell lysates, using POLR2J Antibody. The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-POLR2J Polyclonal Antibody, Western Blot (WB) – Cat# MBS9607309

Immunohistochemistry (IHC) of anti-POLR2J antibody

MBS9607309 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-POLR2J Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9607309

Other Notes

Small volumes of anti-POLR2J antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLR2J purchase

MBS9607309 is an antibody targeting POLR2J. Also known as POLR2J Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for POLR2J. It may not necessarily be applicable to this product.

NCBI GI #
5453932
NCBI GeneID
5439
NCBI Accession #
NP_006225.1
UniProt Primary Accession #
P52435
UniProt Secondary Accession #
O43375; A5D6V8
UniProt Related Accession #
P52435
GenBank Nucleotide #
NM_006234.4
NCBI Official Full Name
DNA-directed RNA polymerase II subunit RPB11-a
NCBI Official Synonym Full Names
RNA polymerase II subunit J
NCBI Official Symbol
POLR2J
NCBI Official Synonym Symbols
RPB11; RPB11A; RPB11m; hRPB14; POLR2J1
UniProt Protein Name
DNA-directed RNA polymerase II subunit RPB11-a
UniProt Synonym Protein Names
DNA-directed RNA polymerase II subunit J-1; RNA polymerase II 13.3 kDa subunit
Protein Family
DNA-directed RNA polymerase
UniProt Gene Name
POLR2J
UniProt Synonym Gene Names
POLR2J1; RNA polymerase II subunit B11-a; RPB11a
NCBI Protein Information
DNA-directed RNA polymerase II subunit RPB11-a

NCBI Summary for POLR2J

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. [provided by RefSeq, Jul 2008]
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UniProt Comments for POLR2J

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft ().
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Research Articles on POLR2J

1. POLR2J interacts with three different subunits of eIF3, eIF3a, eIF3i, and eIF3m.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-POLR2J antibody

 Reference Product  PubMed Publications
 POLR2C antibody  >1 publications with POLR2J and POLR2C

Pathways associated with anti-POLR2J antibody

 Products by Pathway  Pathway Diagram
 Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway antibodies  Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Dual Incision Reaction In TC-NER Pathway antibodies  Dual Incision Reaction In TC-NER Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Eukaryotic Transcription Initiation Pathway antibodies  Eukaryotic Transcription Initiation Pathway Diagram
 Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway antibodies  Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway Diagram
 Formation Of HIV-1 Elongation Complex In The Absence Of HIV-1 Tat Pathway antibodies  Formation Of HIV-1 Elongation Complex In The Absence Of HIV-1 Tat Pathway Diagram
 Formation Of RNA Pol II Elongation Complex Pathway antibodies  Formation Of RNA Pol II Elongation Complex Pathway Diagram

Diseases associated with anti-POLR2J antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >3 publications with POLR2J and Inflammation

Organs/Tissues associated with anti-POLR2J antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1 publications with POLR2J and Blood
 Lung Antibodies  >1 publications with POLR2J and Lung
 Brain Antibodies  >1 publications with POLR2J and Brain
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