• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PP2A-alpha Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9502530

MBS9502530 is an antibody targeting PP2A-alpha. Also known as PP2A-alpha (Phospho-Tyr307) Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$300 / 0.05 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PP2A-alpha, Polyclonal Antibody
Full Product Name
PP2A-alpha (Phospho-Tyr307) Antibody
Product Gene Name
Product Synonym Gene Name
PPP2CA
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Concentration
1 mg/ml (lot specific)
Specificity
PP2A-alpha (Phospho-Tyr307) Antibody detects endogenous levels of PP2A-alpha only when phosphorylated at Tyr307.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Molecular Weight
35 kDa
OMIM
X12646 mRNA
3D Structure
ModBase 3D Structure for P67775
Target Modification
Phospho
Modification Sites
H:Y307; M:Y307; R:Y307
Immunogen
The antiserum was produced against synthesized peptide derived from human PP2A-alpha around the phosphorylation site of Tyr307.
Immunogen Range
260-309
Applications Tested/Suitable for anti-PP2A-alpha antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-PP2A-alpha antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:5000

Immunofluorescence (IF) of anti-PP2A-alpha antibody

Immunofluorescence analysis of A549 cells, using PP2A-alpha (Phospho-Tyr307) Antibody. The picture on the right is blocked with the phospho peptide.
Rabbit Anti-PP2A-alpha Polyclonal Antibody – Cat# MBS9502530

Immunohistochemistry (IHC) of anti-PP2A-alpha antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PP2A-alpha (Phospho-Tyr307) Antibody. The picture on the right is blocked with the phospho peptide.
Rabbit Anti-PP2A-alpha Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9502530

Western Blot (WB) of anti-PP2A-alpha antibody

Western blot analysis of lysates from A549 cells, using PP2A-alpha (Phospho-Tyr307) Antibody. The lane on the right is blocked with the phospho peptide.
Rabbit Anti-PP2A-alpha Polyclonal Antibody, Western Blot (WB) – Cat# MBS9502530

Other Notes

Small volumes of anti-PP2A-alpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PP2A-alpha. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform isoform 1
NCBI Official Synonym Full Names
protein phosphatase 2 catalytic subunit alpha
NCBI Official Symbol
PPP2CA
NCBI Official Synonym Symbols
RP-C; PP2Ac; PP2CA; PP2Calpha
UniProt Protein Name
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
UniProt Synonym Protein Names
Replication protein C; RP-C
UniProt Gene Name
PPP2CA
UniProt Synonym Gene Names
PP2A-alpha; RP-C
NCBI Protein Information
serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

NCBI Summary for PP2A-alpha

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for PP2A-alpha

PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000