PSME3 Antibody – Rabbit Polyclonal

Description: The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome. There are three evolutionarily conserved subunits of PA28: PA28alpha (PSME1), PA28beta (PSME2), and PA28gamma (PSME3) (1, 2). PA28alpha and PA28beta form a heteroheptameric complex and function by binding to the 20S complex at its opening site(s). The PA28alpha/beta complex is present throughout the cell and participates in MHC class I antigen presentation by promoting the generation of antigenic peptides from foreign proteins (2). PA28gamma exists in the form of a homoheptamer and is mainly located in the nucleus. The PA28gamma complex exerts its function by binding and guiding specific nuclear target proteins to the 20S proteasome for further degradation (3, 4).
Function: Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination-and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition (PubMed:25361978).
Subunit Structure: Homoheptamer; the stability of the heptamer is essential for the specific activation of the trypsine-like subunit and inhibition of the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits of the proteasome. Interacts with p53/TP53 and MDM2. Interacts with MAP3K3 (By similarity). Associates with the proteasome. Interacts with human cytomegalovirus UL27. Interacts with CCAR2.
Post-translational Modifications: Phosphorylated by MAP3K3 (By similarity). Phosphorylation at Ser-247 promotes its association with CCAR2. Acetylation at the major site Lys-195 is important for oligomerization and ability to degrade its target substrates. Deacetylated by SIRT1.
Similarity: The C-terminal sequences affect heptamer stability and proteasome affinity. Belongs to the PA28 family.

Small volumes of anti-PSME3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9606735 is an antibody targeting PSME3. Also known as PSME3 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.