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BBC3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS620080

PUMA (p53 upregulated modulator of apoptosis) is one of the pro-apoptotic Bcl-2 family members which are also transcriptional targets of p53. PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2,… More details

$585 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PUMA (Bbc3), Polyclonal Antibody
Full Product Name
PUMA (p53 Upregulated Modulator of Apoptosis, BCL2 Binding Component 3, BCL-2 Binding Component 3, BBC3, JFY1, JFY-1, p53 Up-regulated Modulator of Apoptosis, PUMA alpha, PUMA/JFY1)
Product Gene Name
anti-Bbc3 antibody
Product Synonym Names
Anti -PUMA (p53 Upregulated Modulator of Apoptosis, BCL2 Binding Component 3, BCL-2 Binding Component 3, BBC3, JFY1, JFY-1, p53 Up-regulated Modulator of Apoptosis, PUMA alpha, PUMA/JFY1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
Purity/Purification
Affinity Purified
Purified by immunoaffinitiy chromatography.
Specificity
Recognizes human PUMA alpha at ~25kD (193aa) PUMA beta at ~16kD (131aa). Species Crossreactivity: mouse, rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
20,718 Da
3D Structure
ModBase 3D Structure for Q80ZG6
Immunogen
Synthetic peptide corresponding to aa177 of human PUMA.

Product Categories/Family for anti-Bbc3 antibody

Antibodies; Abs to Apoptosis
Applications Tested/Suitable for anti-Bbc3 antibody
Western Blot (WB)
Application Notes for anti-Bbc3 antibody
Suitable for use in Western Blot.
Dilution: Western Blot: 0.5-4ug/ml

Western Blot (WB) of anti-Bbc3 antibody

Western blot analysis of PUMA in Lysates from Jurkat cells (Lane 1& 2), 3T3 cells (Lane 3) and rat kidney (Lane 4).
Rabbit Anti-BBC3 Polyclonal Antibody – Cat# MBS620080

Other Notes

Small volumes of anti-Bbc3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Bbc3 purchase

MBS620080 is an antibody targeting Bbc3. Also known as PUMA (p53 Upregulated Modulator of Apoptosis, BCL2 Binding Component 3, BCL-2 Binding Component 3, BBC3, JFY1, JFY-1, p53 Up-regulated Modulator of Apoptosis, PUMA alpha, PUMA/JFY1). Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Bbc3. It may not necessarily be applicable to this product.

NCBI GI #
27542559
NCBI GeneID
317673
UniProt Primary Accession #
Q80ZG6
UniProt Related Accession #
Q80ZG6
NCBI Official Full Name
PUMA
NCBI Official Synonym Full Names
Bcl-2 binding component 3
NCBI Official Symbol
Bbc3
NCBI Official Synonym Symbols
Puma
UniProt Protein Name
Bcl-2-binding component 3
UniProt Synonym Protein Names
p53 up-regulated modulator of apoptosis
Protein Family
Bcl-2-binding component
UniProt Gene Name
Bbc3
UniProt Synonym Gene Names
Puma
UniProt Entry Name
BBC3_RAT
NCBI Protein Information
bcl-2-binding component 3; p53 up-regulated modulator of apoptosis

UniProt Comments for Bbc3

PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Cellular Component: mitochondrion; intracellular

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of apoptosis; positive regulation of protein homooligomerization; determination of adult life span; glial cell differentiation; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; reduction of endoplasmic reticulum calcium ion concentration; brain development; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to DNA damage stimulus; negative regulation of growth
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Research Articles on Bbc3

1. Data show that transcriptional activation of PUMA is regulated by nuclear factor-kappaB and endoplasmic reticulum stress.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Bbc3 antibody

 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Measles Pathway antibodies  Measles Pathway Diagram
 Measles Pathway antibodies  Measles Pathway Diagram
 P53 Signaling Pathway antibodies  P53 Signaling Pathway Diagram
 P53 Signaling Pathway antibodies  P53 Signaling Pathway Diagram

Diseases associated with anti-Bbc3 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >155 publications with Bbc3 and Neoplasms
 Leukemia Antibodies  >44 publications with Bbc3 and Leukemia
 Carcinoma Antibodies  >36 publications with Bbc3 and Carcinoma
 Gastrointestinal Neoplasms Antibodies  >34 publications with Bbc3 and Gastrointestinal Neoplasms
 Intestinal Diseases Antibodies  >30 publications with Bbc3 and Intestinal Diseases
 Intestinal Neoplasms Antibodies  >29 publications with Bbc3 and Intestinal Neoplasms
 Colorectal Neoplasms Antibodies  >29 publications with Bbc3 and Colorectal Neoplasms
 Leukemia, Myeloid Antibodies  >25 publications with Bbc3 and Leukemia, Myeloid
 Neuroectodermal Tumors Antibodies  >22 publications with Bbc3 and Neuroectodermal Tumors
 Adenocarcinoma Antibodies  >19 publications with Bbc3 and Adenocarcinoma

Organs/Tissues associated with anti-Bbc3 antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >4 publications with Bbc3 and Kidney
 Mixed Antibodies  >1 publications with Bbc3 and Mixed
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