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Rab5A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9601277

Description: Required for the fusion of plasma membranes and early endosomes.Function: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between… More details

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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Rab5A, Polyclonal Antibody
Full Product Name
Rab5A Antibody
Product Gene Name
anti-Rab5A antibody
Product Synonym Names
RAB 5; RAB 5A; RAB5A; RAB5A member RAS oncogene family; RAB5A_HUMAN; RAS associated protein RAB5A; Ras related protein Rab 5A; Ras-related protein Rab-5A
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
Rab5A antibody detects endogenous levels of total Rab5A
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
Rab5A peptide (MBS9616809) is used for blocking the activity of Rab5A antibody (MBS9601277)
Molecular Weight
Observed: 24 kDa
Predicted: 24 kDa
3D Structure
ModBase 3D Structure for P20339
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human Rab5A
Subcellular Location
Cell Membrane. Early Endosome Membrane. Melanosome. Enriched in stage I melanosomes.
Predicted Cross Reactivity
Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Bovine (92%), Horse (100%), Sheep (92%), Rabbit (92%), Dog (100%), Chicken (83%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-Rab5A antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-Rab5A antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Immunohistochemistry (IHC) of anti-Rab5A antibody

MBS9601277 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-Rab5A Polyclonal Antibody – Cat# MBS9601277

Western Blot (WB) of anti-Rab5A antibody

Western blot analysis of Rab5A expression in HeLa cell lysate, The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-Rab5A Polyclonal Antibody, Western Blot (WB) – Cat# MBS9601277

Immunohistochemistry (IHC) of anti-Rab5A antibody

MBS9601277 at 1/100 staining human colon tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at121 degree
Rabbit Anti-Rab5A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9601277

Other Notes

Small volumes of anti-Rab5A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Rab5A purchase

MBS9601277 is an antibody targeting Rab5A. Also known as Rab5A Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Rab5A. It may not necessarily be applicable to this product.

NCBI GI #
635574573
NCBI GeneID
5868
NCBI Accession #
NP_001278977.1
UniProt Primary Accession #
P20339
UniProt Secondary Accession #
Q6FI44; B4DJA5
UniProt Related Accession #
P20339
GenBank Nucleotide #
NM_001292048.1
NCBI Official Full Name
ras-related protein Rab-5A isoform 2
NCBI Official Synonym Full Names
RAB5A, member RAS oncogene family
NCBI Official Symbol
RAB5A
NCBI Official Synonym Symbols
RAB5
UniProt Protein Name
Ras-related protein Rab-5A
Protein Family
Ras-related protein
UniProt Gene Name
RAB5A
UniProt Synonym Gene Names
RAB5
NCBI Protein Information
ras-related protein Rab-5A

UniProt Comments for Rab5A

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 ().
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Research Articles on Rab5A

1. The authors demonstrate that RABGEF1, the upstream factor of the endosomal Rab GTPase cascade, is recruited to damaged mitochondria via ubiquitin binding downstream of Parkin. RABGEF1 directs the downstream Rab proteins, RAB5 and RAB7A, to damaged mitochondria, whose associations are further regulated by mitochondrial Rab-GAPs.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Rab5A antibody

 Reference Product  PubMed Publications
 EEA1 antibody  >12 publications with Rab5A and EEA1
 RIN1 antibody  >5 publications with Rab5A and RIN1
 CFTR antibody  >2 publications with Rab5A and CFTR
 ALS2 antibody  >1 publications with Rab5A and ALS2
 ZFYVE20 antibody  >1 publications with Rab5A and ZFYVE20
 RABEP1 antibody  >1 publications with Rab5A and RABEP1

Pathways associated with anti-Rab5A antibody

 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 ErbB1 Downstream Signaling Pathway antibodies  ErbB1 Downstream Signaling Pathway Diagram
 Factors Involved In Megakaryocyte Development And Platelet Production Pathway antibodies  Factors Involved In Megakaryocyte Development And Platelet Production Pathway Diagram

Diseases associated with anti-Rab5A antibody

 Disease Name  Pubmed Publications
 Neoplasm Metastasis Antibodies  >10 publications with Rab5A and Neoplasm Metastasis
 Inflammation Antibodies  >8 publications with Rab5A and Inflammation
 Death Antibodies  >5 publications with Rab5A and Death
 Liver Diseases Antibodies  >4 publications with Rab5A and Liver Diseases
 Kidney Diseases Antibodies  >4 publications with Rab5A and Kidney Diseases
 Brain Diseases Antibodies  >3 publications with Rab5A and Brain Diseases
 Carcinoma, Hepatocellular Antibodies  >3 publications with Rab5A and Carcinoma, Hepatocellular
 Weight Loss Antibodies  >2 publications with Rab5A and Weight Loss
 Fibrosis Antibodies  >2 publications with Rab5A and Fibrosis
 Pain Antibodies  >2 publications with Rab5A and Pain

Organs/Tissues associated with anti-Rab5A antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >17 publications with Rab5A and Brain
 Kidney Antibodies  >16 publications with Rab5A and Kidney
 Lung Antibodies  >13 publications with Rab5A and Lung
 Blood Antibodies  >13 publications with Rab5A and Blood
 Nerve Antibodies  >9 publications with Rab5A and Nerve
 Liver Antibodies  >8 publications with Rab5A and Liver
 Heart Antibodies  >7 publications with Rab5A and Heart
 Vascular Antibodies  >7 publications with Rab5A and Vascular
 Muscle Antibodies  >6 publications with Rab5A and Muscle
 Placenta Antibodies  >5 publications with Rab5A and Placenta
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