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RAD21 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2575937

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is nuclear phospho-protein,… More details

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Product Overview

Online Datasheet in PDF
Product Name
Rad21, Polyclonal Antibody
Full Product Name
Rad21 Polyclonal Antibody
Product Gene Name
anti-Rad21 antibody
Product Synonym Names
CDLS4; HR21; HRAD21; MCD1; NXP1; SCC1; hHR21
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Purity/Purification
Affinity purification
Concentration
1mg/mL (lot specific)
Preparation and Storage
Storage: -20 degree C/One year. Avoid freeze/thaw cycles.
Molecular Weight
71690 MW
OMIM
X98294 mRNA
3D Structure
ModBase 3D Structure for O60216
Immunogen
Recombinant fusion protein of human Rad21

Product Categories/Family for anti-Rad21 antibody

Antibodies; Primary Antibodies; Polyclonal Antibody
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-Rad21 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-Rad21 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
IP: 1:50-1:200

Western Blot (WB) of anti-Rad21 antibody

Western blot analysis of extracts of various cell lines using Rad21 Polyclonal Antibody at 1:1000 dilution.
Rabbit Anti-RAD21 Polyclonal Antibody – Cat# MBS2575937

Western Blot (WB) of anti-Rad21 antibody

Western blot analysis of extracts of C6 cells using Rad21 Polyclonal Antibody at 1:1000 dilution.
Rabbit Anti-RAD21 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2575937

Immunohistochemistry (IHC) of anti-Rad21 antibody

Immunohistochemistry of paraffin-embedded human colon carcinoma using Rad21 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Rabbit Anti-RAD21 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2575937

Immunohistochemistry (IHC) of anti-Rad21 antibody

Immunohistochemistry of paraffin-embedded mouse stomach using Rad21 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Rabbit Anti-RAD21 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2575937

Immunohistochemistry (IHC) of anti-Rad21 antibody

Immunohistochemistry of paraffin-embedded rat stomach using Rad21 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Rabbit Anti-RAD21 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2575937

Immunofluorescence (IF) of anti-Rad21 antibody

Immunofluorescence analysis of U2OS cells using Rad21 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Rabbit Anti-RAD21 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS2575937

Immunoprecipitation (IP) of anti-Rad21 antibody

Immunoprecipitation analysis of 300ug extracts of Jurkat cells usingug Rad21 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using Rad21 Polyclonal Antibody at a dilution of 1:1000.
Rabbit Anti-RAD21 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS2575937

Other Notes

Small volumes of anti-Rad21 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Rad21 purchase

MBS2575937 is an antibody targeting Rad21. Also known as Rad21 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Rad21. It may not necessarily be applicable to this product.

NCBI GI #
5453994
NCBI GeneID
5885
NCBI Accession #
NP_006256.1
UniProt Primary Accession #
O60216
UniProt Secondary Accession #
Q15001; Q99568; A8K0E0
UniProt Related Accession #
O60216
GenBank Nucleotide #
NM_006265.2
NCBI Official Full Name
double-strand-break repair protein rad21 homolog
NCBI Official Synonym Full Names
RAD21 cohesin complex component
NCBI Official Symbol
RAD21
NCBI Official Synonym Symbols
HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21
UniProt Protein Name
Double-strand-break repair protein rad21 homolog
UniProt Synonym Protein Names
Nuclear matrix protein 1; NXP-1; SCC1 homolog
Protein Family
Cofilin
UniProt Gene Name
RAD21
UniProt Synonym Gene Names
; hHR21; NXP-1
NCBI Protein Information
double-strand-break repair protein rad21 homolog

NCBI Summary for Rad21

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008]
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UniProt Comments for Rad21

Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.
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Research Articles on Rad21

1. This study together with previous reports suggests incomplete penetrance associated with RAD21 variants and these individuals may therefore be underdiagnosed.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Rad21 antibody

 Reference Product  PubMed Publications
 SMC3 antibody  >92 publications with Rad21 and SMC3
 SMC1A antibody  >34 publications with Rad21 and SMC1A
 NIPBL antibody  >34 publications with Rad21 and NIPBL
 STAG2 antibody  >13 publications with Rad21 and STAG2
 ESPL1 antibody  >12 publications with Rad21 and ESPL1
 WAPAL antibody  >7 publications with Rad21 and WAPAL
 STAG1 antibody  >7 publications with Rad21 and STAG1
 PDS5B antibody  >4 publications with Rad21 and PDS5B
 CDCA5 antibody  >3 publications with Rad21 and CDCA5
 PDS5A antibody  >2 publications with Rad21 and PDS5A

Pathways associated with anti-Rad21 antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cohesin Loading Onto Chromatin Pathway antibodies  Cohesin Loading Onto Chromatin Pathway Diagram
 Establishment Of Sister Chromatid Cohesion Pathway antibodies  Establishment Of Sister Chromatid Cohesion Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Meiosis Pathway antibodies  Meiosis Pathway Diagram
 Meiotic Synapsis Pathway antibodies  Meiotic Synapsis Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram

Diseases associated with anti-Rad21 antibody

 Disease Name  Pubmed Publications
 Leukemia, Myeloid Antibodies  >12 publications with Rad21 and Leukemia, Myeloid
 Neoplasm Metastasis Antibodies  >6 publications with Rad21 and Neoplasm Metastasis
 Neoplasms, Experimental Antibodies  >4 publications with Rad21 and Neoplasms, Experimental
 Ovarian Neoplasms Antibodies  >3 publications with Rad21 and Ovarian Neoplasms
 Infertility, Male Antibodies  >3 publications with Rad21 and Infertility, Male
 Necrosis Antibodies  >3 publications with Rad21 and Necrosis
 Adenocarcinoma Antibodies  >3 publications with Rad21 and Adenocarcinoma
 Liver Neoplasms Antibodies  >2 publications with Rad21 and Liver Neoplasms
 Cell Transformation, Neoplastic Antibodies  >2 publications with Rad21 and Cell Transformation, Neoplastic
 Fibrosis Antibodies  >2 publications with Rad21 and Fibrosis

Organs/Tissues associated with anti-Rad21 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >23 publications with Rad21 and Blood
 Testis Antibodies  >13 publications with Rad21 and Testis
 Bone Antibodies  >10 publications with Rad21 and Bone
 Brain Antibodies  >9 publications with Rad21 and Brain
 Ovary Antibodies  >6 publications with Rad21 and Ovary
 Bone Marrow Antibodies  >6 publications with Rad21 and Bone Marrow
 Liver Antibodies  >5 publications with Rad21 and Liver
 Lymph Node Antibodies  >5 publications with Rad21 and Lymph Node
 Lymph Antibodies  >5 publications with Rad21 and Lymph
 Embryonic Tissue Antibodies  >4 publications with Rad21 and Embryonic Tissue
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