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RNF216 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758652

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Product Overview

Online Datasheet in PDF
Product Name
RNF216, Polyclonal Antibody
Full Product Name
Anti-RNF216 Antibody Picoband
Product Gene Name
anti-RNF216 antibody
Product Synonym Names
lymphocyte antigen 9; T-lymphocyte surface antigen Ly-9; Cell surface molecule Ly-9; Lymphocyte antigen 9; SLAM family member 3; SLAMF3; Signaling lymphocytic activation molecule 3; CD229; LY9; CDABP0070
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Increased surface expression on T-cells of systemic lupus erythematosus (SLE) patients.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
56,697 Da
OMIM
phenotype 609948
3D Structure
ModBase 3D Structure for Q9NWF9
Immunogen
E coli-derived human RNF216 recombinant protein (Position: K538-R813).
Subcellular Localization
Cell membrane. Membrane. Single-pass type I membrane protein
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. Promotes T-cell differentiation into a helper T-cell Th17 phenotype leading to increased IL-17 secretion; the costimulatory activity requires SH2D1A (PubMed:22184727). Promotes recruitment of RORC to the IL-17 promoter (PubMed:22989874). May be involved in the maintenance of peripheral cell tolerance by serving as a negative regulator of the immune response. May disable autoantibody responses and inhibit IFN-gamma secretion by CD4+ T-cells. May negatively regulate the size of thymic innate CD8+ T-cells and the development of invariant natural killer T (iNKT) cells (By similarity)

Product Categories/Family for anti-RNF216 antibody

Primary Antibodies; Adaptive Immunity; B Cells; Hematopoietic Progenitors; Immunology; Lymphoid; Stem Cells; T Cells
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-RNF216 antibody
Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-RNF216 antibody
WB: 0.25-0.5ug/ml, Human, Mouse, Rat
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-RNF216 antibody

Figure 1. Western blot analysis of RNF216 using anti-RNF216 antibody (MBS1758652).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U2OS whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: rat testis tissue lysates,
Lane 5: rat heart tissue lysates,
Lane 6: mouse testis tissue lysates,
Lane 7: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF216 antigen affinity purified polyclonal antibody (#MBS1758652) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for RNF216 at approximately 50 kDa. The expected band size for RNF216 is at 99 kDa.
Rabbit Anti-RNF216 Polyclonal Antibody – Cat# MBS1758652

Flow Cytometry (FC/FACS) of anti-RNF216 antibody

Figure 2. Flow Cytometry analysis of HepG2 cells using anti-RNF216 antibody (MBS1758652).
Overlay histogram showing HepG2 cells stained with MBS1758652 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNF216 Antibody (MBS1758652, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Rabbit Anti-RNF216 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758652

Product References and Citations for anti-RNF216 antibody

1. Chen, D., Li, X., Zhai, Z., Shu, H.-B. A novel zinc finger protein interacts with receptor-interacting protein (RIP) and inhibits tumor necrosis factor (TNF)- and IL1-induced NF-kappa-B activation. J. Biol. Chem. 277: 15985-15991, 2002. 2. Chuang, T.-H., Ulevitch, R. J. Triad3A, an E3 ubiquitin-protein ligase regulating Toll-like receptors. Nature Immun. 5: 495-502, 2004. Note: Erratum: Nature Immun. 5: 968 only, 2004. 3. Margolin, D. H., Kousi, M., Chan, Y.-M., Lim, E. T., Schmahmann, J. D., Hadjivassiliou, M., Hall, J. E., Adam, I., Dwyer, A., Plummer, L., Aldrin, S. V., O'Rourke, J., and 9 others. Ataxia, dementia, and hypogonadotropism caused by disordered ubiquitination. New Eng. J. Med. 368: 1992-2003, 2013.

Other Notes

Small volumes of anti-RNF216 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RNF216 purchase

MBS1758652 is an antibody targeting RNF216. Also known as Anti-RNF216 Antibody Picoband. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RNF216. It may not necessarily be applicable to this product.

NCBI GI #
31324099
NCBI GeneID
54476
NCBI Accession #
AAP47174
UniProt Primary Accession #
Q9NWF9
UniProt Secondary Accession #
Q6Y691; Q75ML7; Q7Z2H7; Q7Z7C1; Q8NHW7; Q9NYT1
UniProt Related Accession #
Q9NWF9
NCBI Official Full Name
E3 ubiquitin ligase
NCBI Official Synonym Full Names
ring finger protein 216
NCBI Official Symbol
RNF216
NCBI Official Synonym Symbols
ZIN; CAHH; U7I1; TRIAD3; UBCE7IP1
UniProt Protein Name
E3 ubiquitin-protein ligase RNF216
UniProt Synonym Protein Names
RING finger protein 216; Triad domain-containing protein 3; Ubiquitin-conjugating enzyme 7-interacting protein 1; Zinc finger protein inhibiting NF-kappa-B
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
RNF216
UniProt Synonym Gene Names
TRIAD3; UBCE7IP1; ZIN
UniProt Entry Name
RN216_HUMAN
NCBI Protein Information
E3 ubiquitin-protein ligase RNF216; triad domain-containing protein 3; zinc finger protein inhibiting NF-kappa-B; ubiquitin-conjugating enzyme 7-interacting protein 1

NCBI Summary for RNF216

This gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known. [provided by RefSeq, Jul 2008]
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UniProt Comments for RNF216

TRIAD3: Isoform 1 acts as an E3 ubiquitin ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Promotes degradation of TRAF3, TLR4 and TLR9. Contributes to the regulation of antiviral responses. Down- regulates activation of NF-kappa-B, IRF3 activation and IFNB production. Isoform 3/ZIN inhibits TNF and IL-1 mediated activation of NF-kappa-B. Promotes TNF and RIP mediated apoptosis. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19; Ligase

Chromosomal Location of Human Ortholog: 7p22.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; zinc ion binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; viral reproduction; apoptosis; regulation of interferon-beta production; regulation of defense response to virus by host

Disease: Gordon Holmes Syndrome
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Research Articles on RNF216

1. Loss of Triad3A mimics and occludes Arc-dependent forms of synaptic plasticity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RNF216 antibody

 Reference Product  PubMed Publications
 TIRAP antibody  >2 publications with RNF216 and TIRAP
 TICAM1 antibody  >2 publications with RNF216 and TICAM1
 RIPK1 antibody  >2 publications with RNF216 and RIPK1
 TLR4 antibody  >1 publications with RNF216 and TLR4
 TLR9 antibody  >1 publications with RNF216 and TLR9
 TRAF3 antibody  >1 publications with RNF216 and TRAF3

Pathways associated with anti-RNF216 antibody

 Products by Pathway  Pathway Diagram
 Gastric Cancer Network 1 Pathway antibodies  Gastric Cancer Network 1 Pathway Diagram
 TNF-alpha/NF-kB Signaling Pathway antibodies  TNF-alpha/NF-kB Signaling Pathway Diagram

Diseases associated with anti-RNF216 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >5 publications with RNF216 and Necrosis
 Nervous System Diseases Antibodies  >3 publications with RNF216 and Nervous System Diseases
 Weight Gain Antibodies  >1 publications with RNF216 and Weight Gain
 Neoplasm Invasiveness Antibodies  >1 publications with RNF216 and Neoplasm Invasiveness
 Ovarian Diseases Antibodies  >1 publications with RNF216 and Ovarian Diseases
 Liver Diseases Antibodies  >1 publications with RNF216 and Liver Diseases
 Prostatic Diseases Antibodies  >1 publications with RNF216 and Prostatic Diseases
 Weight Loss Antibodies  >1 publications with RNF216 and Weight Loss
 Immune System Diseases Antibodies  >1 publications with RNF216 and Immune System Diseases
 Prenatal Injuries Antibodies  >1 publications with RNF216 and Prenatal Injuries

Organs/Tissues associated with anti-RNF216 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4 publications with RNF216 and Blood
 Nerve Antibodies  >2 publications with RNF216 and Nerve
 Brain Antibodies  >2 publications with RNF216 and Brain
 Stomach Antibodies  >1 publications with RNF216 and Stomach
 Kidney Antibodies  >1 publications with RNF216 and Kidney
 Liver Antibodies  >1 publications with RNF216 and Liver
 Prostate Antibodies  >1 publications with RNF216 and Prostate
 Bone Marrow Antibodies  >1 publications with RNF216 and Bone Marrow
 Eye Antibodies  >1 publications with RNF216 and Eye
 Bone Antibodies  >1 publications with RNF216 and Bone
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